##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511689_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1948649 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.949734918910487 31.0 31.0 34.0 28.0 34.0 2 31.14388584090824 31.0 31.0 34.0 28.0 34.0 3 31.260240299817976 33.0 31.0 34.0 28.0 34.0 4 34.81614185007151 37.0 35.0 37.0 32.0 37.0 5 34.464685533413146 37.0 35.0 37.0 31.0 37.0 6 34.41621759485675 37.0 35.0 37.0 30.0 37.0 7 34.24392694630998 37.0 35.0 37.0 30.0 37.0 8 34.14975914082013 37.0 35.0 37.0 30.0 37.0 9 35.65434616495839 39.0 35.0 39.0 30.0 39.0 10 35.455206658561906 39.0 35.0 39.0 30.0 39.0 11 35.45044438480198 39.0 35.0 39.0 30.0 39.0 12 35.28146166908458 39.0 35.0 39.0 30.0 39.0 13 35.275704860136436 39.0 35.0 39.0 30.0 39.0 14 36.28613465021151 40.0 36.0 41.0 29.0 41.0 15 36.21374911541278 40.0 36.0 41.0 29.0 41.0 16 36.05566266680146 39.0 36.0 41.0 28.0 41.0 17 36.03871913310196 39.0 36.0 41.0 28.0 41.0 18 35.97025015792993 39.0 36.0 41.0 27.0 41.0 19 35.99484771244077 39.0 36.0 41.0 27.0 41.0 20 36.105117956081365 40.0 36.0 41.0 28.0 41.0 21 36.048994457185465 40.0 36.0 41.0 28.0 41.0 22 35.94834523816244 40.0 36.0 41.0 27.0 41.0 23 35.8416964779188 40.0 36.0 41.0 27.0 41.0 24 35.69566504793834 39.0 36.0 41.0 27.0 41.0 25 35.177420869535766 39.0 35.0 40.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09905733112360693 1101 2 0.1329282228103139 1101 3 0.11474754489295336 1101 4 0.2058231408398541 1101 5 0.3723688944982726 1101 6 0.17878517443869413 1101 7 0.03529535720308985 1101 8 -0.13928482171652234 1101 9 0.017622364695114356 1101 10 0.09696449946045504 1101 11 0.0687426093128991 1101 12 0.1523740371945408 1101 13 0.053526146460384894 1101 14 -0.09817769020079936 1101 15 0.0018218465825370345 1101 16 -0.11645842281041041 1101 17 -0.08673106415123044 1101 18 -0.21180805361379385 1101 19 -0.13791728268016357 1101 20 -0.24454683476157157 1101 21 -0.026527046242151187 1101 22 0.0686896043996228 1101 23 -0.03995469952765518 1101 24 -0.43279959099726284 1101 25 -0.1859488406018457 1102 1 0.4494214618431869 1102 2 0.4594969963872728 1102 3 0.3834938593089987 1102 4 0.5344283135590047 1102 5 0.6202056349860641 1102 6 0.596903355901091 1102 7 0.6544674913029525 1102 8 0.558738411806722 1102 9 0.6428509621069622 1102 10 0.5852183900109651 1102 11 0.6973040690993173 1102 12 0.6836851664806787 1102 13 0.732110059703146 1102 14 0.8935029228150384 1102 15 0.8010464892674491 1102 16 0.7545655410305656 1102 17 0.8646470872096117 1102 18 0.8421717800648025 1102 19 1.0209941525301787 1102 20 0.9082557346832871 1102 21 0.9006415032645734 1102 22 1.065854565333261 1102 23 1.0896906287272827 1102 24 0.7943016976387653 1102 25 0.7965205487371065 1103 1 0.3839615075806222 1103 2 0.4174440352386135 1103 3 0.4853310009103211 1103 4 0.4760309074704452 1103 5 0.5754511074257564 1103 6 0.6078981769963718 1103 7 0.437928757809523 1103 8 0.6230555671674907 1103 9 0.5732523431482264 1103 10 0.878627867470307 1103 11 0.4935514334636153 1103 12 0.7732247981605411 1103 13 0.7937938088989185 1103 14 0.9194592365560155 1103 15 0.8428217133362992 1103 16 0.8749078710917857 1103 17 0.9308096653231672 1103 18 0.7220039911959404 1103 19 0.931641802275692 1103 20 0.7906984195599946 1103 21 1.0128026926927234 1103 22 1.0418570624189343 1103 23 1.1181019853709913 1103 24 0.8969822426659348 1103 25 0.8288132173700191 1104 1 0.1737208403506365 1104 2 0.24333384745860087 1104 3 0.34069722968704497 1104 4 0.37071422246548735 1104 5 0.3993543055826052 1104 6 0.5558741771422646 1104 7 0.6121696438831634 1104 8 0.5119291910360388 1104 9 0.6524500379950666 1104 10 0.6614644459713475 1104 11 0.6575832630573544 1104 12 0.7478729765910686 1104 13 0.6820370414628556 1104 14 0.7624243249141429 1104 15 0.825791421727132 1104 16 0.6676756962113899 1104 17 0.6181866417549173 1104 18 0.7247067103283484 1104 19 0.6536410606388614 1104 20 0.7510208492108816 1104 21 0.6520705390887969 1104 22 0.6643876245431741 1104 23 0.7184527431171475 1104 24 1.01765810481875 1104 25 0.9357347558409117 1105 1 0.4183542287741915 1105 2 0.3974742232824653 1105 3 0.36767640745667407 1105 4 0.4079592740086184 1105 5 0.46371264894033004 1105 6 0.6029851029274198 1105 7 0.4381229956215478 1105 8 0.4395134632652926 1105 9 0.4090798984578896 1105 10 0.6433619781557951 1105 11 0.7741102129525856 1105 12 0.6390239093571779 1105 13 0.7108662902719374 1105 14 0.8048775694784283 1105 15 0.6049705969198982 1105 16 0.7388217329363798 1105 17 0.7652096027821997 1105 18 0.6776215531806571 1105 19 0.572994010152243 1105 20 0.8130447882750786 1105 21 0.9057235362741594 1105 22 0.7808501528447493 1105 23 0.9339236473972008 1105 24 0.9841565335175133 1105 25 1.0487571710415935 1106 1 0.1273165167588317 1106 2 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0.7218687023362378 1107 15 0.6099529631646732 1107 16 0.7892124151178521 1107 17 0.9164886452742849 1107 18 0.7724508270367849 1107 19 0.7269314904211939 1107 20 0.6115155692690735 1107 21 0.7490118039333424 1107 22 0.6806434507832009 1107 23 0.6039957638417164 1107 24 0.8787646432224605 1107 25 0.8065165818348987 1108 1 0.2789597706686813 1108 2 0.32163938942627723 1108 3 0.2842188077884913 1108 4 0.3336337411995629 1108 5 0.42115979597181763 1108 6 0.40760660358193945 1108 7 0.4903817900021892 1108 8 0.5408791310830026 1108 9 0.4671663176013823 1108 10 0.5235780095554006 1108 11 0.49211122025055687 1108 12 0.38706884055258683 1108 13 0.4569103414532094 1108 14 0.5543939457514 1108 15 0.7528212008049096 1108 16 0.7074177373210873 1108 17 0.591196683472468 1108 18 0.7017698212974821 1108 19 0.5889001010131523 1108 20 0.6637073774697768 1108 21 0.7695940183836498 1108 22 0.7910959533009958 1108 23 0.6071064785628693 1108 24 0.7517433745630413 1108 25 0.8272602472505994 1109 1 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-0.5144244597402832 2313 19 -0.4010809420012791 2313 20 -0.5963690660766119 2313 21 -0.546532274611323 2313 22 -0.3803642777678178 2313 23 -0.41385619953230446 2313 24 -0.44028618167692457 2313 25 -0.3053354475039143 2314 1 -0.7068992757112298 2314 2 -0.7027183509717112 2314 3 -0.6547244266563723 2314 4 -0.6870060166405452 2314 5 -0.7798201126138906 2314 6 -0.790537382369088 2314 7 -0.864305392000773 2314 8 -0.8610891289793656 2314 9 -0.9293909380857954 2314 10 -2.125311483666735 2314 11 -1.3749532398887823 2314 12 -1.1410144365688666 2314 13 -1.2250788392042224 2314 14 -1.143146151315186 2314 15 -1.2631798550008284 2314 16 -1.0557258442598965 2314 17 -1.1150978027865008 2314 18 -1.0268365595728852 2314 19 -1.146011341948011 2314 20 -1.0622821515679917 2314 21 -0.9731475393919453 2314 22 -0.9373420141129998 2314 23 -0.8903246607741622 2314 24 -1.0492150268688718 2314 25 -1.127604057717285 2315 1 -0.5786015178337536 2315 2 -0.6452497815853455 2315 3 -0.691971098073008 2315 4 -0.7647818468799485 2315 5 -0.7423822283386414 2315 6 -0.9113850471820513 2315 7 -0.8376604113003694 2315 8 -0.9265450085091942 2315 9 -1.0321363601692113 2315 10 -1.2548065982540777 2315 11 -1.1241127000311408 2315 12 -1.0508894425442747 2315 13 -1.136940713712434 2315 14 -1.32887378073859 2315 15 -1.3041289992094676 2315 16 -1.344789606919214 2315 17 -1.3518398088370205 2315 18 -1.24532684300042 2315 19 -1.3212810696897321 2315 20 -1.4041005555021187 2315 21 -1.4434006612074342 2315 22 -1.4152861420192266 2315 23 -1.2963716156714469 2315 24 -1.2820181997882756 2315 25 -1.3815411835907412 2316 1 -1.1897984309209342 2316 2 -1.217747869400121 2316 3 -1.1623053943718986 2316 4 -1.19591888813909 2316 5 -1.387614532010602 2316 6 -1.3493901380574513 2316 7 -1.4785510847699328 2316 8 -2.171355181522884 2316 9 -1.6793749990021425 2316 10 -1.8023837626010817 2316 11 -1.5824027714683453 2316 12 -1.6935000470659531 2316 13 -1.9312445671940424 2316 14 -1.8572997180671251 2316 15 -1.988593016798184 2316 16 -1.7438008066191415 2316 17 -1.8849553545357054 2316 18 -1.891564103101672 2316 19 -1.7470592848253474 2316 20 -1.7949171329711362 2316 21 -1.876534434333955 2316 22 -1.9941751275687167 2316 23 -1.9181946672667465 2316 24 -2.0019074493341904 2316 25 -1.8702812277056324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29861.0 3 1.0 4 538.0 5 4785.0 6 6215.0 7 4952.0 8 3935.0 9 3574.0 10 3363.0 11 3179.0 12 3015.0 13 2898.0 14 2826.0 15 2869.0 16 2904.0 17 3014.0 18 3331.0 19 3566.0 20 4460.0 21 5802.0 22 7677.0 23 9262.0 24 11232.0 25 13666.0 26 15752.0 27 18014.0 28 21124.0 29 25686.0 30 32756.0 31 42691.0 32 54872.0 33 72309.0 34 94670.0 35 129337.0 36 191625.0 37 350719.0 38 762169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.103678292062895 20.319990847686643 14.111310888521627 24.465019971728832 2 14.039008564395129 21.037036428828383 42.68762614508822 22.23632886168828 3 18.686433523944025 25.403497500062866 30.55958256207249 25.35048641392062 4 12.107054682500543 18.03983169878208 39.34223146395272 30.510882154764662 5 12.30414157503977 38.50008239105516 36.707242764088996 12.488533269816076 6 30.383665811544308 38.887044306080774 18.42399529109655 12.305294591278368 7 26.944154642524126 31.998733481504367 22.978227479653853 18.078884396317655 8 23.677840390958043 39.3285296633719 20.934298583274874 16.059331362395177 9 25.741988423774625 15.630008277529713 20.855577376941667 37.77242592175399 10 14.150111179591605 30.240848916351794 35.33607129862792 20.272968605428684 11 33.824100697457574 22.604378725978872 25.63596625149013 17.935554325073422 12 22.864918207434997 24.931118944458444 33.24138929073425 18.962573557372313 13 29.05330821507619 22.55516514261932 26.279899561183157 22.11162708112133 14 19.856320969040603 23.329137263817138 28.41902261515542 28.395519151986836 15 21.93427343764834 32.71045734762905 23.853808459091404 21.50146075563121 16 20.893193181532435 28.991213912818576 29.472727002143532 20.642865903505452 17 20.474441523332317 29.44337333198539 29.101136223096102 20.981048921586186 18 20.32952060632777 28.205900600877836 31.779042813764818 19.685535979029574 19 22.802669952361867 27.146551277320853 28.455509432432418 21.59526933788486 20 22.55732048203653 28.334759107463686 29.45912783677307 19.64879257372672 21 21.92616525603123 27.49792291993068 28.092847916684843 22.483063907353248 22 21.89634972742654 29.316362259185723 28.62470357668313 20.16258443670461 23 21.303631387694757 28.61469664367467 28.977255524211902 21.10441644441867 24 22.30227198433376 27.550831370862582 29.815785192715566 20.331111452088088 25 21.926164728873125 28.177601652051106 29.3631145925707 20.533119026505076 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 142.0 1 142.0 2 335.5 3 529.0 4 529.0 5 529.0 6 1465.5 7 2402.0 8 2402.0 9 2402.0 10 3581.0 11 4760.0 12 4760.0 13 4760.0 14 7519.0 15 10278.0 16 10278.0 17 10278.0 18 18367.5 19 26457.0 20 26457.0 21 26457.0 22 43139.0 23 59821.0 24 59821.0 25 59821.0 26 86953.5 27 114086.0 28 114086.0 29 114086.0 30 148613.5 31 183141.0 32 183141.0 33 183141.0 34 200461.0 35 217781.0 36 217781.0 37 217781.0 38 226893.5 39 236006.0 40 236006.0 41 236006.0 42 243545.5 43 251085.0 44 251085.0 45 251085.0 46 249680.5 47 248276.0 48 248276.0 49 248276.0 50 232232.5 51 216189.0 52 216189.0 53 216189.0 54 185681.0 55 155173.0 56 155173.0 57 155173.0 58 130040.5 59 104908.0 60 104908.0 61 104908.0 62 83727.0 63 62546.0 64 62546.0 65 62546.0 66 47677.5 67 32809.0 68 32809.0 69 32809.0 70 23538.5 71 14268.0 72 14268.0 73 14268.0 74 9842.5 75 5417.0 76 5417.0 77 5417.0 78 3627.0 79 1837.0 80 1837.0 81 1837.0 82 1183.5 83 530.0 84 530.0 85 530.0 86 345.5 87 161.0 88 161.0 89 161.0 90 101.0 91 41.0 92 41.0 93 41.0 94 23.0 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4185463877794308 2 0.0 3 0.0 4 0.0 5 0.03191955041672461 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.002104021812034902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1948649.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.25185510712586 #Duplication Level Percentage of deduplicated Percentage of total 1 86.61845933996173 63.449628331733486 2 9.75458081622669 14.29082281161974 3 2.0055452460113417 4.407297293148236 4 0.6327163391779234 1.8539058240548938 5 0.28603078655886455 1.0476142866593594 6 0.15467960087167074 0.679834062664781 7 0.09840086886206828 0.5045632331809662 8 0.06398250172535304 0.3749469556621593 9 0.05423433575677545 0.3575489134217878 >10 0.28439481011511075 3.789781436088909 >50 0.024058097182541184 1.2731918149247123 >100 0.017887909499456206 2.700722055597335 >500 0.002724230194041648 1.3904884172868863 >1k 0.002095561687724332 2.990867671288049 >5k 2.0955616877243321E-4 0.8887868926687437 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6630 0.34023572228759513 No Hit GTCCTACAGTGGACATTTCTAAATT 5446 0.27947567776444093 No Hit CTGTAGGACGTGGAATATGGCAAGA 5294 0.27167540177836025 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4551 0.23354642113587415 No Hit CTTTAGGACGTGAAATATGGCGAGG 4181 0.21455890722238843 No Hit TATCAACGCAGAGTACTTTTTTTTT 4171 0.2140457311706726 No Hit GGTATCAACGCAGAGTACTTTTTTT 4077 0.2092218762845438 No Hit TTGTAGAACAGTGTATATCAATGAG 2684 0.13773645228052872 No Hit GTCCTACAGTGTGCATTTCTCATTT 2442 0.12531759182900565 No Hit CTGAAGGACCTGGAATATGGCGAGA 2418 0.12408596930488765 No Hit GATATACACTGTTCTACAAATCCCG 2292 0.11761995105326818 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2003 0.10278916315868071 No Hit CTGTAGGACCTGGAATATGGCGAGA 1994 0.10232730471213647 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.131760517158298E-5 2 0.0 0.0 0.0 0.0 5.131760517158298E-5 3 0.0 0.0 0.0 0.0 5.131760517158298E-5 4 0.0 0.0 0.0 0.0 5.131760517158298E-5 5 0.0 0.0 0.0 0.0 5.131760517158298E-5 6 0.0 0.0 0.0 0.0 5.131760517158298E-5 7 0.0 0.0 0.0 0.0 5.131760517158298E-5 8 0.0 0.0 0.0 0.0 5.131760517158298E-5 9 0.0 0.0 0.0 0.0 5.131760517158298E-5 10 0.0 0.0 0.0 5.131760517158298E-5 5.131760517158298E-5 11 0.0 0.0 0.0 5.131760517158298E-5 5.131760517158298E-5 12 0.0 0.0 0.0 5.131760517158298E-5 1.0263521034316596E-4 13 0.0 0.0 0.0 5.131760517158298E-5 1.0263521034316596E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 815 0.0 13.988486 4 GGTATCA 1100 0.0 13.00666 1 AGCGTAC 70 1.0510287E-4 12.263423 1 ACTGTTC 980 0.0 11.726012 8 ACCTTTT 740 0.0 11.678867 15 GACAGTC 155 2.5465852E-11 11.641607 7 GCGTACA 75 2.073289E-4 11.400616 2 CCAACGA 260 0.0 11.324047 19 CACCTTT 765 0.0 11.297205 14 TCCAACG 345 0.0 11.286409 18 GGCGAGG 1000 0.0 11.112177 19 GGACCTG 1580 0.0 11.059915 6 GATATAC 1040 0.0 11.005636 1 AGGACCT 1685 0.0 10.994659 5 GTTTAGG 235 0.0 10.958804 1 CTATACT 270 0.0 10.907997 4 CTAGGAC 375 0.0 10.893922 3 AATCCCG 545 0.0 10.804596 19 GTATCAA 2380 0.0 10.78059 1 TGTAGGA 3090 0.0 10.761099 2 >>END_MODULE