FastQCFastQC Report
Thu 26 May 2016
SRR1511688_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511688_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869876
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64270.3437126312119092No Hit
TATCAACGCAGAGTACTTTTTTTTT38520.2060029649024855No Hit
GGTATCAACGCAGAGTACTTTTTTT38430.2055216495639283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT400.00544223914.1849818
GTCAACG500.001111222813.8391852
GGTATCA10150.013.8294471
TTCAACG707.525466E-512.70945552
AAGCCGT550.00317575212.0357426
TGTTCCG550.00317575212.03574210
GTATCAA22000.010.8736471
GTATAGG1957.6397555E-1110.13859751
GTATAAT2400.09.8851321
AAGGCGT1151.0838105E-59.8678146
TCGAACT1252.7246842E-69.83492119
GCGACTA1107.187299E-59.45665516
GTGTCGA800.00468974569.4566559
TAGACCG900.00116129489.4566545
CGCCCTA1357.2554467E-69.10640716
GTATTAG1853.4513505E-89.0836351
AGACCGT850.00772008448.900386
GCGTGCG850.00772008448.900389
GTTTAGG1905.3183612E-88.8445921
GTGGTAT2801.8189894E-128.8260111
Produced by FastQC (version 0.11.5)