##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511688_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1869876 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.11804739993454 31.0 29.0 34.0 22.0 34.0 2 26.463520575695927 31.0 25.0 34.0 10.0 34.0 3 28.12425690259675 31.0 27.0 34.0 16.0 34.0 4 32.607142933542114 35.0 32.0 37.0 25.0 37.0 5 33.05380196333874 35.0 35.0 37.0 27.0 37.0 6 33.299628424558634 37.0 35.0 37.0 28.0 37.0 7 33.33631641884275 37.0 35.0 37.0 27.0 37.0 8 33.36089986715697 37.0 35.0 37.0 27.0 37.0 9 34.923862330978096 39.0 35.0 39.0 28.0 39.0 10 34.80852634078409 39.0 35.0 39.0 27.0 39.0 11 34.81864358920057 39.0 35.0 39.0 27.0 39.0 12 34.7144639537595 39.0 35.0 39.0 27.0 39.0 13 34.69808693196768 39.0 35.0 39.0 27.0 39.0 14 35.82045012610462 40.0 36.0 41.0 27.0 41.0 15 35.746005082689976 40.0 36.0 41.0 27.0 41.0 16 35.68380309710376 40.0 36.0 41.0 27.0 41.0 17 35.653339044942015 40.0 36.0 41.0 27.0 41.0 18 35.59087073153514 40.0 36.0 41.0 27.0 41.0 19 35.617637747101945 40.0 36.0 41.0 27.0 41.0 20 35.538557102182175 40.0 36.0 41.0 26.0 41.0 21 35.45715651733056 40.0 36.0 41.0 26.0 41.0 22 35.34977025214506 40.0 36.0 41.0 25.0 41.0 23 35.230783217710695 40.0 36.0 41.0 25.0 41.0 24 35.10263086964056 40.0 36.0 41.0 25.0 41.0 25 34.987814700012194 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.142018060663844 1101 2 3.6967876029326945 1101 3 2.3460736526136934 1101 4 1.450162392957786 1101 5 1.0443694890016886 1101 6 0.776136764660798 1101 7 0.6166935783547629 1101 8 0.5102542933311298 1101 9 0.45786224017617627 1101 10 0.7819812658142311 1101 11 0.4641322795293803 1101 12 0.592653128913085 1101 13 0.5452218403661604 1101 14 0.5939836244697645 1101 15 0.5233475026277787 1101 16 0.6089252577052946 1101 17 0.7250571970381117 1101 18 0.8333447678667838 1101 19 0.7546431427588445 1101 20 0.82955051225278 1101 21 0.8722985345758261 1101 22 0.7691974711659668 1101 23 0.8705120386264369 1101 24 0.8618369400923243 1101 25 0.7820825800794111 1102 1 1.3971629425146723 1102 2 4.000384307241447 1102 3 2.578768492124901 1102 4 1.5865747746695433 1102 5 1.0682859656036428 1102 6 0.8259120865346219 1102 7 0.7163462080468861 1102 8 0.6856778440490459 1102 9 0.7379527295232791 1102 10 0.6646433462038814 1102 11 0.6915624809670717 1102 12 0.7248207319961537 1102 13 0.8480528774705007 1102 14 0.8191129611902639 1102 15 0.895356687543746 1102 16 0.876499290229539 1102 17 0.9915124746562185 1102 18 0.9155693903642614 1102 19 0.9766209592706829 1102 20 1.1358235749834051 1102 21 0.8549253834573847 1102 22 0.7759965341314725 1102 23 0.8793006001301436 1102 24 0.5912666841385104 1102 25 0.5406285386545733 1103 1 1.6923057308990792 1103 2 4.284942750651968 1103 3 2.898843540701474 1103 4 1.9316489319328554 1103 5 1.438459504295551 1103 6 1.3205708918183277 1103 7 1.2454988365296913 1103 8 1.0980149565506068 1103 9 1.1998936532803413 1103 10 0.9765561935932183 1103 11 1.1574630638859489 1103 12 1.3664860616929317 1103 13 1.3448419214478946 1103 14 1.3809589600850387 1103 15 1.261461381199382 1103 16 1.3437011905094067 1103 17 1.4258316004561706 1103 18 1.415197267077815 1103 19 1.5921307013246206 1103 20 1.3434394011416657 1103 21 1.5004968935203067 1103 22 1.5765919313897925 1103 23 1.501845032096199 1103 24 1.6465303346011027 1103 25 1.3022468070111657 1104 1 1.6570464090220014 1104 2 4.132483202229235 1104 3 2.732478981638387 1104 4 1.8657961333369926 1104 5 1.3639275958446646 1104 6 1.1137053388142135 1104 7 0.9053933161616143 1104 8 0.9088454790916174 1104 9 1.0220791459470462 1104 10 1.0549545186133358 1104 11 1.1734456776284077 1104 12 0.9906165385322794 1104 13 1.1315935567542539 1104 14 1.392520709196937 1104 15 1.3895553555896285 1104 16 1.2626392717686485 1104 17 1.2935351060258853 1104 18 1.1412266759562684 1104 19 1.410622911013938 1104 20 1.5326845807379783 1104 21 1.311211760018189 1104 22 1.2668117760041469 1104 23 1.3291856300309348 1104 24 1.3938418573075353 1104 25 1.4942610726583823 1105 1 1.3271400994291014 1105 2 3.669668512889423 1105 3 2.1589013082510142 1105 4 1.4211034671391687 1105 5 1.0349709739449011 1105 6 0.9073634730462103 1105 7 0.6972085607796927 1105 8 0.5040000568670777 1105 9 0.6021837338139733 1105 10 0.29452620450884126 1105 11 0.4362145176980263 1105 12 0.5315236339711049 1105 13 0.5103451947590685 1105 14 0.48182636692421 1105 15 0.6214482797061152 1105 16 0.4919216282890062 1105 17 0.5940063823499884 1105 18 0.6365193630967738 1105 19 0.5424570701076021 1105 20 0.4052477234081593 1105 21 0.5495886562141834 1105 22 0.446172271176259 1105 23 0.7570144854078649 1105 24 0.6681529538549356 1105 25 0.6326683918931693 1106 1 1.249080228265779 1106 2 3.7037221430070737 1106 3 2.216716655426165 1106 4 1.3906806340305238 1106 5 0.7974002262422673 1106 6 0.7927050170633976 1106 7 0.5768091429149607 1106 8 0.670028629201461 1106 9 0.5122022425754409 1106 10 0.3958929995492184 1106 11 0.5666575051268623 1106 12 0.4645524131325942 1106 13 0.684247539151869 1106 14 0.728812277016317 1106 15 0.9400563785724856 1106 16 0.6569372007094358 1106 17 0.6947964769922592 1106 18 0.801659484686958 1106 19 0.5180107100382187 1106 20 0.7924152337381898 1106 21 0.7069625709380105 1106 22 0.6635132143380602 1106 23 0.8095357380252892 1106 24 0.5410686423702913 1106 25 0.5059622569784921 1107 1 1.3190026672410333 1107 2 3.7666218142655374 1107 3 2.3413492249517915 1107 4 1.4711086085004013 1107 5 1.0457855162032885 1107 6 0.858389223734072 1107 7 0.840837890247947 1107 8 0.6157313333066128 1107 9 0.7109313833604531 1107 10 0.664511003108295 1107 11 0.7347445468498748 1107 12 0.6516130609090851 1107 13 0.7484452358244624 1107 14 0.8103040065874865 1107 15 1.0306495479522155 1107 16 0.9191703843743113 1107 17 0.8534234240303817 1107 18 0.8186185186691262 1107 19 0.8425170635711936 1107 20 0.8980200174311435 1107 21 0.9813434715738225 1107 22 0.912974468928077 1107 23 0.9625104368315007 1107 24 1.092184593202525 1107 25 0.9291376193725398 1108 1 1.2524292522893674 1108 2 3.802576393713732 1108 3 2.358581487191394 1108 4 1.3166886786532217 1108 5 1.002594695999477 1108 6 0.8017929449055714 1108 7 0.7159068218149542 1108 8 0.5537980515093253 1108 9 0.6055454043559365 1108 10 0.3831077956186135 1108 11 0.527801280991838 1108 12 0.5123253411779132 1108 13 0.5761326856208839 1108 14 0.6237852584602877 1108 15 0.5906231588080217 1108 16 0.6348260966923505 1108 17 0.5624635944518772 1108 18 0.7217240274029777 1108 19 0.4983823530334135 1108 20 0.7617168142221473 1108 21 0.5206332057434793 1108 22 0.6394678603108161 1108 23 0.6328123256193905 1108 24 0.6783263895276903 1108 25 0.642350825024046 1109 1 1.1560288204383937 1109 2 3.1826622431884424 1109 3 2.0081133593440654 1109 4 1.3277583314019878 1109 5 0.9510103745505347 1109 6 0.7114412617629142 1109 7 0.704859155048382 1109 8 0.6032702722208185 1109 9 0.6081828543522931 1109 10 0.6946069271723161 1109 11 0.6830275225344664 1109 12 0.6170014703027462 1109 13 0.5431155261403546 1109 14 0.716794931135297 1109 15 0.49303664107517164 1109 16 0.46854727046608247 1109 17 0.5847894915088787 1109 18 0.5583809083789646 1109 19 0.7635995456086349 1109 20 0.6859756841195193 1109 21 0.7014369553948896 1109 22 0.644995020520355 1109 23 0.5126057036729819 1109 24 0.6376601332660243 1109 25 0.7338871476023741 1110 1 0.9104304552826328 1110 2 3.4464150594520113 1110 3 1.9773048472666908 1110 4 1.2035353692368105 1110 5 0.46490466876849723 1110 6 0.35328889975608035 1110 7 0.26906450985600117 1110 8 0.14648016223210902 1110 9 0.24943485311192148 1110 10 0.26015928847005654 1110 11 0.21467262955179933 1110 12 0.3627115178147875 1110 13 0.28098517223955355 1110 14 0.1932149538863115 1110 15 0.05102651138999903 1110 16 0.2421830888932064 1110 17 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-2.10774959282557 2315 19 -2.1521891960183694 2315 20 -2.2177585155249986 2315 21 -2.2376522610690657 2315 22 -2.145026426098134 2315 23 -2.098434255343413 2315 24 -2.0850895325486576 2315 25 -2.0867930480612955 2316 1 -0.3468195745305742 2316 2 2.0074097829016075 2316 3 0.6326254592660021 2316 4 -0.30128198034588394 2316 5 -0.6645868650551705 2316 6 -0.8569956498786482 2316 7 -1.1931331184066067 2316 8 -1.2761363686876734 2316 9 -1.407953324131853 2316 10 -1.445867006003894 2316 11 -1.3260320772178886 2316 12 -1.4920184055071886 2316 13 -1.313662079447404 2316 14 -1.5445511773816207 2316 15 -1.534145561146694 2316 16 -1.6526173329830272 2316 17 -1.5267358738504342 2316 18 -1.7521618622378412 2316 19 -1.6863232184336425 2316 20 -1.5614833787811122 2316 21 -1.8134311649729682 2316 22 -1.6283490344207436 2316 23 -1.689580088676749 2316 24 -1.6538469059935323 2316 25 -1.8413377713560166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72707.0 3 1.0 4 644.0 5 3214.0 6 3793.0 7 3351.0 8 3150.0 9 3061.0 10 2856.0 11 2921.0 12 2782.0 13 2806.0 14 2785.0 15 2892.0 16 3052.0 17 3258.0 18 3707.0 19 4759.0 20 5987.0 21 7856.0 22 9438.0 23 11038.0 24 13129.0 25 14946.0 26 16790.0 27 19386.0 28 22300.0 29 26985.0 30 33668.0 31 42597.0 32 54687.0 33 71803.0 34 98599.0 35 139116.0 36 218312.0 37 277749.0 38 663751.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.185644395671154 18.687977170678696 13.327514765684997 24.798863667965147 2 17.060109228524635 21.14214801191787 38.55548909671253 23.242253662844973 3 18.552834519508245 24.448359142531377 29.972629201080714 27.026177136879664 4 12.547623478776131 17.157715270959145 38.43976819853295 31.854893051731775 5 13.673206137733196 37.165245182033466 35.51700754488533 13.644541135348012 6 31.564766861545902 37.059409287032935 18.259980875737213 13.11584297568395 7 27.574876622834882 32.061537770419 22.2914781514924 18.072107455253715 8 25.439066547728302 35.482299361027145 20.894433641589067 18.184200449655485 9 26.050871822516577 15.977209183924495 20.41819885382774 37.55372013973119 10 14.88740429846685 28.924859188523733 34.36120897856328 21.826527534446136 11 34.360834622188854 23.091531203138604 23.84430839264208 18.70332578203047 12 22.696157392254886 25.1241258778657 31.328280591868126 20.85143613801129 13 28.456967199964062 21.8647653641204 25.943859378910687 23.73440805700485 14 21.521159691872615 21.31189447856435 28.132239784884135 29.0347060446789 15 23.539742742299488 28.778111489745843 24.392633522222866 23.289512245731803 16 23.046020163903915 28.313588708556075 26.077397645619282 22.562993481920728 17 22.2932964538825 27.620227223623385 27.344593973076293 22.74188234941782 18 21.940599269684196 27.623275554100914 28.19545253268131 22.240672643533582 19 22.994906076609396 27.212621164516342 28.25579249949863 21.536680259375625 20 23.081209663100655 26.83439971420565 28.29578004102946 21.78861058166424 21 23.54002272879203 26.583969516678923 27.983260913162646 21.8927468413664 22 22.91157159642177 26.861272724258406 27.937394343863698 22.289761335456127 23 22.99659226553375 26.726649638690304 27.588209623801518 22.688548471974432 24 22.505408115515742 26.78189718564075 28.070913831138444 22.641780867705062 25 23.021098725263066 26.695834376183235 27.869922925370457 22.413143973183246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 285.0 1 285.0 2 484.0 3 683.0 4 683.0 5 683.0 6 1636.0 7 2589.0 8 2589.0 9 2589.0 10 3622.0 11 4655.0 12 4655.0 13 4655.0 14 7517.5 15 10380.0 16 10380.0 17 10380.0 18 17699.0 19 25018.0 20 25018.0 21 25018.0 22 37019.5 23 49021.0 24 49021.0 25 49021.0 26 66818.0 27 84615.0 28 84615.0 29 84615.0 30 105006.0 31 125397.0 32 125397.0 33 125397.0 34 144180.5 35 162964.0 36 162964.0 37 162964.0 38 180598.0 39 198232.0 40 198232.0 41 198232.0 42 212151.5 43 226071.0 44 226071.0 45 226071.0 46 234503.5 47 242936.0 48 242936.0 49 242936.0 50 239353.0 51 235770.0 52 235770.0 53 235770.0 54 215707.5 55 195645.0 56 195645.0 57 195645.0 58 167512.5 59 139380.0 60 139380.0 61 139380.0 62 113789.5 63 88199.0 64 88199.0 65 88199.0 66 67664.0 67 47129.0 68 47129.0 69 47129.0 70 33864.0 71 20599.0 72 20599.0 73 20599.0 74 13912.0 75 7225.0 76 7225.0 77 7225.0 78 4749.5 79 2274.0 80 2274.0 81 2274.0 82 1423.5 83 573.0 84 573.0 85 573.0 86 368.0 87 163.0 88 163.0 89 163.0 90 108.0 91 53.0 92 53.0 93 53.0 94 36.0 95 19.0 96 19.0 97 19.0 98 10.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.330019744624777 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 5.3479482061912124E-5 20 0.0 21 5.3479482061912124E-5 22 6.952332668048577E-4 23 6.417537847429455E-4 24 5.3479482061912124E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1869876.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.72322714694527 #Duplication Level Percentage of deduplicated Percentage of total 1 65.77516218113084 34.67878816302925 2 17.078640220328932 18.008820553947153 3 7.2613970489792425 11.48532858052472 4 3.734061536664388 7.874870983129123 5 2.0983564215647785 5.531606112470555 6 1.203358948271282 3.8066980301409585 7 0.8033761404707327 2.9649607916932257 8 0.5344339614999913 2.254166651776468 9 0.370544882534929 1.7582689809024494 >10 1.10706349754492 8.425576396444105 >50 0.02049779454719747 0.7413300849022294 >100 0.01155971132057894 1.129048619171454 >500 8.25416075838609E-4 0.2783014422496608 >1k 6.190620568789554E-4 0.7126170628363077 >5k 1.031770094798259E-4 0.34961754678236256 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6427 0.3437126312119092 No Hit TATCAACGCAGAGTACTTTTTTTTT 3852 0.2060029649024855 No Hit GGTATCAACGCAGAGTACTTTTTTT 3843 0.2055216495639283 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGTAT 40 0.005442239 14.184981 8 GTCAACG 50 0.0011112228 13.839185 2 GGTATCA 1015 0.0 13.829447 1 TTCAACG 70 7.525466E-5 12.7094555 2 AAGCCGT 55 0.003175752 12.035742 6 TGTTCCG 55 0.003175752 12.035742 10 GTATCAA 2200 0.0 10.873647 1 GTATAGG 195 7.6397555E-11 10.1385975 1 GTATAAT 240 0.0 9.885132 1 AAGGCGT 115 1.0838105E-5 9.867814 6 TCGAACT 125 2.7246842E-6 9.834921 19 GCGACTA 110 7.187299E-5 9.456655 16 GTGTCGA 80 0.0046897456 9.456655 9 TAGACCG 90 0.0011612948 9.456654 5 CGCCCTA 135 7.2554467E-6 9.106407 16 GTATTAG 185 3.4513505E-8 9.083635 1 AGACCGT 85 0.0077200844 8.90038 6 GCGTGCG 85 0.0077200844 8.90038 9 GTTTAGG 190 5.3183612E-8 8.844592 1 GTGGTAT 280 1.8189894E-12 8.826011 1 >>END_MODULE