FastQCFastQC Report
Thu 26 May 2016
SRR1511688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511688_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869876
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82010.43858523238974134No Hit
TATCAACGCAGAGTACTTTTTTTTT51380.27477757883410453No Hit
GGTATCAACGCAGAGTACTTTTTTT49790.2662743411862605No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24540.13123864897993234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11600.013.4045061
CGCCCTA1052.0030711E-812.662744516
TCGAACT701.0937413E-412.21115819
GCACCGG550.003075973112.0871656
ATCCCGT600.00588997311.0799026
GTCTAGG1951.8189894E-1210.76240351
GTATCAA25750.010.7063581
AGACGAT803.7822535E-410.6841916
GTCTTAA2201.8189894E-129.9730121
GTATTAC1159.772131E-69.9541591
TACACCG1002.754456E-49.5003615
TGCACCG900.00111334899.50036055
GGACAAT1201.7066664E-59.4970596
GCGACTA900.0011168959.49705816
CGACTAA900.0011168959.49705817
GTATTAT2451.8189894E-129.3447221
TGGTATC3700.09.2435942
GTCCTAC1554.011854E-79.231681
GCGCCAC1252.763998E-59.11717713
ACTATAC1151.1101484E-49.0873013
Produced by FastQC (version 0.11.5)