FastQCFastQC Report
Thu 26 May 2016
SRR1511687_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511687_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1710514
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50520.29534981882638783No Hit
GTCCTACAGTGGACATTTCTAAATT33530.1960229498267772No Hit
CTGTAGGACGTGGAATATGGCAAGA32670.19099522131943966No Hit
GGTATCAACGCAGAGTACTTTTTTT30700.17947821532007338No Hit
TATCAACGCAGAGTACTTTTTTTTT30190.17649665539130344No Hit
GTCCTAAAGTGTGTATTTCTCATTT29060.16989045398049943No Hit
CTTTAGGACGTGAAATATGGCGAGG24630.14399180597177222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA250.006194546418.91289112
CGCTTAA454.99668E-415.378951
CGAACGG500.001552482813.23902414
GCGAACG500.001552482813.23902413
CCAACGT1207.9671736E-1012.60896219
TAGCGGC604.254117E-412.6085944
GGTATCA8800.012.2457391
GCGCGAT550.003176086812.03547613
TCCAACG2300.011.92369418
AGGACCG658.339437E-411.6387015
CGTGCGC752.1644593E-411.34773410
CGGTAGG700.00106889711.29882051
GGCGAGG6550.011.2614419
TAGGACC5300.011.0622574
AGCGAAC600.006080480811.0325212
GCGGCGA600.006080480811.032526
AATGTCC4400.010.9608798
GTCCTAA10950.010.6539111
AGGACCT10900.010.5842785
CAAGTCG1356.0830644E-810.5071616
Produced by FastQC (version 0.11.5)