##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511687_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1710514 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.61851174559226 31.0 30.0 34.0 25.0 34.0 2 26.960235929083304 31.0 26.0 34.0 10.0 34.0 3 28.63287818749218 31.0 28.0 34.0 16.0 34.0 4 33.13104598968497 35.0 33.0 37.0 28.0 37.0 5 33.59334679517385 35.0 35.0 37.0 28.0 37.0 6 33.84506645370924 37.0 35.0 37.0 30.0 37.0 7 33.89280064354925 37.0 35.0 37.0 30.0 37.0 8 33.910000152001096 37.0 35.0 37.0 30.0 37.0 9 35.526886655122375 39.0 37.0 39.0 30.0 39.0 10 35.40383183066611 39.0 35.0 39.0 30.0 39.0 11 35.41871507628701 39.0 35.0 39.0 30.0 39.0 12 35.33618023588232 39.0 35.0 39.0 30.0 39.0 13 35.33625039023358 39.0 35.0 39.0 30.0 39.0 14 36.516082300407945 40.0 37.0 41.0 30.0 41.0 15 36.46395878665711 40.0 37.0 41.0 29.0 41.0 16 36.406005446316136 40.0 37.0 41.0 29.0 41.0 17 36.39158638865277 40.0 37.0 41.0 29.0 41.0 18 36.34177796849368 40.0 37.0 41.0 29.0 41.0 19 36.377123484519856 40.0 37.0 41.0 29.0 41.0 20 36.31175249077178 40.0 37.0 41.0 29.0 41.0 21 36.22930651254535 40.0 37.0 41.0 29.0 41.0 22 36.131202083116534 40.0 37.0 41.0 28.0 41.0 23 36.031539642470044 40.0 37.0 41.0 28.0 41.0 24 35.93620572529661 40.0 36.0 41.0 27.0 41.0 25 35.84022638809153 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2547942053168946 1101 2 3.7344790549512368 1101 3 2.4518104248709953 1101 4 1.6041377734189908 1101 5 1.0273461719704144 1101 6 0.9326128153911739 1101 7 0.7079554459778521 1101 8 0.6013944906160233 1101 9 0.7650836957097837 1101 10 0.8765814741713314 1101 11 0.7537968838315692 1101 12 0.6194815198207309 1101 13 0.754826966119964 1101 14 0.8719204198614534 1101 15 0.7520149681976562 1101 16 0.7122777107035247 1101 17 0.8504197883496687 1101 18 0.7900156859001513 1101 19 0.8921899792377701 1101 20 0.9656820074751664 1101 21 1.0927932320283915 1101 22 1.0966486506808977 1101 23 1.0534135289514168 1101 24 0.8785946775775884 1101 25 0.8746346046594837 1102 1 1.44982106119512 1102 2 4.158362991740724 1102 3 2.7217322503343766 1102 4 1.707399750158892 1102 5 1.2535422096545474 1102 6 0.943819059536267 1102 7 1.0064259086025658 1102 8 0.999067253887489 1102 9 0.936984224645812 1102 10 1.1405131911066988 1102 11 1.0977971620036513 1102 12 1.055925335196278 1102 13 1.0740801558946842 1102 14 1.1522409722784843 1102 15 1.2333365310338706 1102 16 1.1030153985208173 1102 17 1.1029088148700978 1102 18 1.187143150075272 1102 19 1.1561548313770373 1102 20 1.0513389640271456 1102 21 1.1195672949361892 1102 22 0.6341548379643456 1102 23 1.102377951560264 1102 24 0.703166681193828 1102 25 0.5364783946722866 1103 1 1.304366956642859 1103 2 3.921952987153503 1103 3 2.485570804410031 1103 4 1.5951761201111836 1103 5 0.9136921935891706 1103 6 0.7563989570007621 1103 7 0.6535635434153306 1103 8 0.6428871357513941 1103 9 0.7384048485482637 1103 10 0.5350275216250537 1103 11 0.7683126484208316 1103 12 0.9112076109428173 1103 13 0.8406362846844502 1103 14 0.9236454095558173 1103 15 0.739595965653848 1103 16 0.7830314841058268 1103 17 0.981088395472824 1103 18 0.850016771130413 1103 19 0.9297178894973257 1103 20 0.8075562649225603 1103 21 0.8625859287526936 1103 22 0.8815414356004112 1103 23 0.8545851726946196 1103 24 0.9126761718910643 1103 25 0.8480533472339573 1104 1 1.4772912012786783 1104 2 4.178137875004424 1104 3 2.6142601441657867 1104 4 1.867956152223286 1104 5 1.1977663433483272 1104 6 1.0841778700548232 1104 7 0.9044057562309789 1104 8 0.8497116382571122 1104 9 0.9204750086222191 1104 10 0.9240373985286681 1104 11 0.9996855866780194 1104 12 0.9466975150930637 1104 13 0.7703691703945665 1104 14 1.0034496230526315 1104 15 1.2090199913435242 1104 16 0.9427971630821332 1104 17 0.9538363154845015 1104 18 1.0199197536633733 1104 19 1.0350852811189881 1104 20 1.0937533164478879 1104 21 1.0712114335451872 1104 22 1.2204276322626981 1104 23 1.2473924514373849 1104 24 1.1712463076376736 1104 25 1.1217091639030556 1105 1 1.588825272550455 1105 2 3.9832466508672972 1105 3 2.5779692656454003 1105 4 1.6139383109267555 1105 5 1.2387867534179193 1105 6 0.9005769172735398 1105 7 0.9164220095587083 1105 8 0.7546872115605581 1105 9 0.8588031577044646 1105 10 0.5864613861985575 1105 11 0.6826851663333358 1105 12 0.7900823172975393 1105 13 0.7928062354545773 1105 14 0.848977996318709 1105 15 0.8195723242565123 1105 16 0.8312578608543078 1105 17 0.7804648708999409 1105 18 0.7541688779452898 1105 19 0.7845041779477171 1105 20 0.7982091569037237 1105 21 0.9136027277397574 1105 22 0.8934024582867011 1105 23 0.9948205958491343 1105 24 0.8730676144425829 1105 25 1.035689080768222 1106 1 0.9905383348309229 1106 2 3.6373663833749887 1106 3 2.05459130635559 1106 4 1.1857883102681797 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-1.8863580692550599 2315 15 -2.08090308557955 2315 16 -2.230895144610173 2315 17 -2.1169713768231873 2315 18 -2.0321571694135443 2315 19 -2.0186454881117797 2315 20 -2.045438991244424 2315 21 -1.8984039510702004 2315 22 -2.1569569831219155 2315 23 -1.9691460101010776 2315 24 -1.9876767692854074 2315 25 -1.8340216053277132 2316 1 -0.6338431351202907 2316 2 1.9446414344764662 2316 3 0.5106541873412205 2316 4 -0.42181150083401064 2316 5 -0.7829587884483402 2316 6 -0.8776245696265548 2316 7 -1.361005410891046 2316 8 -1.2126139860935226 2316 9 -1.586653632621477 2316 10 -1.619629819695831 2316 11 -1.448385833647528 2316 12 -1.6856531217638278 2316 13 -1.5516841639649215 2316 14 -1.8099826315362009 2316 15 -1.9666404542638958 2316 16 -1.9696535237997708 2316 17 -1.9652367595145321 2316 18 -2.0740065941259047 2316 19 -2.001443136936196 2316 20 -1.8121701068021991 2316 21 -2.0552948965429394 2316 22 -1.7982706399503314 2316 23 -1.9245732237078812 2316 24 -2.0272055336675976 2316 25 -2.145162425737915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53243.0 3 1.0 4 444.0 5 2292.0 6 2915.0 7 2539.0 8 2282.0 9 2283.0 10 2166.0 11 2017.0 12 2028.0 13 2009.0 14 2112.0 15 2130.0 16 2208.0 17 2451.0 18 2814.0 19 3443.0 20 4447.0 21 5746.0 22 7244.0 23 8586.0 24 10176.0 25 11510.0 26 13076.0 27 14674.0 28 17366.0 29 21548.0 30 26788.0 31 34841.0 32 45183.0 33 60560.0 34 84886.0 35 122409.0 36 202397.0 37 257245.0 38 672455.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60994063772644 19.308465174795412 13.133186866637747 24.948407320840403 2 15.776689682908836 21.426436549067258 41.05321769999346 21.743656068030447 3 18.605226265321416 25.081642126284848 30.142401640676432 26.170729967717303 4 11.66707784911436 17.465451905099872 39.796400380236584 31.071069865549184 5 12.456723534563295 38.221961351967884 36.748427665602264 12.572887447866549 6 30.251959352568875 38.533972829219756 18.86123118547992 12.352836632731448 7 26.764645013136402 32.02446749924292 23.16748065201454 18.04340683560614 8 23.643945621023857 38.567413069989485 21.327273556369605 16.46136775261705 9 25.909112699457594 15.729540945002496 20.86998411003944 37.491362245500476 10 14.075710575885378 29.827993223089667 35.61338872409112 20.482907476933836 11 33.733018262346874 22.82775820601293 25.455915590284555 17.98330794135564 12 22.608058162634155 24.91590247142087 33.190140507473195 19.285898858471782 13 28.630809218749455 22.48581420555459 26.458421269863912 22.424955305832047 14 20.087763093432734 22.9102480307089 28.692778895700354 28.309209980158013 15 22.15573798285194 31.881995704215228 24.156540080934736 21.8057262319981 16 21.08085639755068 28.95147306599069 28.97380553447677 20.99386500198186 17 20.763524881994535 28.97661170852738 29.000464187957537 21.25939922152055 18 20.402463820816433 28.26892969013992 31.215646291114833 20.112960197928807 19 22.52096300934281 27.315957259605746 28.51360965979212 21.649470071259323 20 22.383739624463757 28.195150697392712 29.431971910197753 19.989137767945774 21 22.1509244898162 27.49605234926873 28.11615944007376 22.236863720841317 22 21.848203997327087 28.87629472906349 28.64940107910409 20.626100194505327 23 21.520303115870732 28.355259657842936 28.734212958659484 21.390224267626852 24 22.04238024359929 27.562943068574707 29.617705555172304 20.776971132653692 25 21.98877062684082 27.953761267081124 29.166613076537228 20.890855029540827 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 241.0 1 241.0 2 392.5 3 544.0 4 544.0 5 544.0 6 1465.0 7 2386.0 8 2386.0 9 2386.0 10 3419.5 11 4453.0 12 4453.0 13 4453.0 14 7178.5 15 9904.0 16 9904.0 17 9904.0 18 17498.5 19 25093.0 20 25093.0 21 25093.0 22 39163.0 23 53233.0 24 53233.0 25 53233.0 26 75308.5 27 97384.0 28 97384.0 29 97384.0 30 125337.0 31 153290.0 32 153290.0 33 153290.0 34 168391.5 35 183493.0 36 183493.0 37 183493.0 38 192854.0 39 202215.0 40 202215.0 41 202215.0 42 209189.0 43 216163.0 44 216163.0 45 216163.0 46 217064.0 47 217965.0 48 217965.0 49 217965.0 50 205576.0 51 193187.0 52 193187.0 53 193187.0 54 167920.5 55 142654.0 56 142654.0 57 142654.0 58 120455.5 59 98257.0 60 98257.0 61 98257.0 62 78854.0 63 59451.0 64 59451.0 65 59451.0 66 44771.5 67 30092.0 68 30092.0 69 30092.0 70 21634.0 71 13176.0 72 13176.0 73 13176.0 74 9095.5 75 5015.0 76 5015.0 77 5015.0 78 3311.0 79 1607.0 80 1607.0 81 1607.0 82 1045.0 83 483.0 84 483.0 85 483.0 86 314.0 87 145.0 88 145.0 89 145.0 90 101.0 91 57.0 92 57.0 93 57.0 94 39.0 95 21.0 96 21.0 97 21.0 98 13.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.350914403506782 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 5.8461959387646056E-5 20 0.0 21 1.7538587816293816E-4 22 0.0012277011471405672 23 7.600054720393987E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1710514.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.06130415631907 #Duplication Level Percentage of deduplicated Percentage of total 1 87.51425153297075 67.4396235539487 2 9.213389034404674 14.199915493815068 3 1.9209518618868242 4.44093167095524 4 0.5516734218668266 1.7005069342974737 5 0.2290368247901041 0.8824938209073884 6 0.1429834430968637 0.6611094358683093 7 0.08627187458811204 0.46537562174392205 8 0.05510400535993043 0.3397109213818428 9 0.04253032740699828 0.29496982465606747 >10 0.20687970001356049 2.9924244175327006 >50 0.017854622965198713 0.9447359352945294 >100 0.015536103104670858 2.279897033319137 >500 0.0019993138300994674 1.019645332369276 >1k 0.001461037029688072 2.0392906647385516 >5k 7.689668577305643E-5 0.29936933917182434 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5052 0.29534981882638783 No Hit GTCCTACAGTGGACATTTCTAAATT 3353 0.1960229498267772 No Hit CTGTAGGACGTGGAATATGGCAAGA 3267 0.19099522131943966 No Hit GGTATCAACGCAGAGTACTTTTTTT 3070 0.17947821532007338 No Hit TATCAACGCAGAGTACTTTTTTTTT 3019 0.17649665539130344 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2906 0.16989045398049943 No Hit CTTTAGGACGTGAAATATGGCGAGG 2463 0.14399180597177222 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.846195938764605E-5 0.0 0.0 0.0 0.0 7 5.846195938764605E-5 0.0 0.0 0.0 0.0 8 5.846195938764605E-5 0.0 0.0 0.0 0.0 9 5.846195938764605E-5 0.0 0.0 0.0 0.0 10 5.846195938764605E-5 0.0 0.0 0.0 0.0 11 5.846195938764605E-5 0.0 0.0 0.0 0.0 12 5.846195938764605E-5 0.0 0.0 0.0 0.0 13 5.846195938764605E-5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGCGA 25 0.0061945464 18.912891 12 CGCTTAA 45 4.99668E-4 15.37895 1 CGAACGG 50 0.0015524828 13.239024 14 GCGAACG 50 0.0015524828 13.239024 13 CCAACGT 120 7.9671736E-10 12.608962 19 TAGCGGC 60 4.254117E-4 12.608594 4 GGTATCA 880 0.0 12.245739 1 GCGCGAT 55 0.0031760868 12.035476 13 TCCAACG 230 0.0 11.923694 18 AGGACCG 65 8.339437E-4 11.638701 5 CGTGCGC 75 2.1644593E-4 11.347734 10 CGGTAGG 70 0.001068897 11.2988205 1 GGCGAGG 655 0.0 11.26144 19 TAGGACC 530 0.0 11.062257 4 AGCGAAC 60 0.0060804808 11.03252 12 GCGGCGA 60 0.0060804808 11.03252 6 AATGTCC 440 0.0 10.960879 8 GTCCTAA 1095 0.0 10.653911 1 AGGACCT 1090 0.0 10.584278 5 CAAGTCG 135 6.0830644E-8 10.507161 6 >>END_MODULE