FastQCFastQC Report
Thu 26 May 2016
SRR1511687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511687_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1710514
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62380.36468570266013606No Hit
TATCAACGCAGAGTACTTTTTTTTT38690.22618932087080257No Hit
GGTATCAACGCAGAGTACTTTTTTT37020.2164261736530657No Hit
GTCCTACAGTGGACATTTCTAAATT36080.21093074947062693No Hit
CTGTAGGACGTGGAATATGGCAAGA35090.20514301549125No Hit
GTCCTAAAGTGTGTATTTCTCATTT30920.1807643784266016No Hit
CTTTAGGACGTGAAATATGGCGAGG26720.15621035548379025No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19670.11499467411549978No Hit
TTGTAGAACAGTGTATATCAATGAG17920.10476383122266172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2650.013.26019218
GGTATCA9800.012.4587231
CCAACGA2100.012.21099819
TAGGACC5300.012.1889614
CCAACGT802.8746654E-511.87180219
TGCCGGT1552.5465852E-1111.64168610
CCTACCG752.0736031E-411.4002633
GCCGGTT1803.6379788E-1211.08002611
GATATAC8600.010.9806081
ACTGTTC8000.010.8030248
ATTTAGA6100.010.7896881
GTATCAA22150.010.7660381
CCTACAC2300.010.7393783
AATGTCC5050.010.7195728
CACCTTT5150.010.69583514
TCTATAC2750.010.3638753
GCTATAC2053.6379788E-1210.1953573
CTACCGT750.002647297510.1335674
GTCCTAC16500.010.0590031
TGTAGGA18400.010.0165352
Produced by FastQC (version 0.11.5)