FastQCFastQC Report
Thu 26 May 2016
SRR1511686_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511686_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851989
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56900.30723724600956054No Hit
GTCCTACAGTGGACATTTCTAAATT35780.19319769177894686No Hit
TATCAACGCAGAGTACTTTTTTTTT34960.18877001969234158No Hit
GGTATCAACGCAGAGTACTTTTTTT33640.18164254755292825No Hit
CTGTAGGACGTGGAATATGGCAAGA32820.17721487546632295No Hit
GTCCTAAAGTGTGTATTTCTCATTT32400.17494704342196418No Hit
CTTTAGGACGTGAAATATGGCGAGG30580.16511977122974272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG454.992187E-415.3808881
TATTGCG400.005443502714.1844755
TAGGACC5050.013.6695274
GTATCGC707.6184424E-613.5090236
TATCGCG655.6945308E-513.093367
TCGCGCG655.6945308E-513.093369
GGTATCA10850.012.6672111
ACGAAAT951.0996409E-611.94482112
GCGCCTT803.0054836E-511.82039513
GGCGAGG8600.011.65577419
ATCGCGC658.3409634E-411.6385428
AATCCCG2900.011.41310419
CGCCCTA1251.9605068E-811.34788516
AGGACCT11550.010.88909155
CGCGCGC700.001550089310.80721910
GTCCTAA11300.010.6752311
GGACCTG11000.010.5738816
GTCTTAG2350.010.5188451
TGGCGAG20100.010.25638118
TTATGCT2151.8189894E-1210.116064
Produced by FastQC (version 0.11.5)