FastQCFastQC Report
Thu 26 May 2016
SRR1511686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851989
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69560.37559618334666134No Hit
TATCAACGCAGAGTACTTTTTTTTT45660.2465457408224347No Hit
GGTATCAACGCAGAGTACTTTTTTT43780.2363944926238763No Hit
GTCCTACAGTGGACATTTCTAAATT35740.1929817077747222No Hit
CTGTAGGACGTGGAATATGGCAAGA33900.18304644358038843No Hit
GTCCTAAAGTGTGTATTTCTCATTT32480.17537901143041346No Hit
CTTTAGGACGTGAAATATGGCGAGG31980.17267921137760536No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21220.1145795142411753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12250.013.1567821
TAGGACC5850.013.1540524
TCCAACG1353.7289283E-1011.95959818
GCGGAGC658.0436596E-411.68901813
GACGTGA10550.011.4328017
CTAGACT1803.6379788E-1211.0835074
TTAGGAC11700.010.961713
TAGGACT2050.010.6587034
GGACGTG19900.010.6427346
CCTAGAC1355.6865247E-810.555723
AGGACGT20250.010.5088065
ATTAGAC1106.044944E-610.3637983
TTTAACC1201.5235128E-610.2918273
GGCGAGG9700.010.280889519
ACGTGAA12600.010.2510838
GTATTAT2053.6379788E-1210.234531
TGTAGGA20450.010.2202082
GTCCTAA9450.010.1926871
CGAAATC1502.4554538E-810.13048313
ACGAAAT1701.5661499E-910.05599312
Produced by FastQC (version 0.11.5)