FastQCFastQC Report
Thu 26 May 2016
SRR1511685_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511685_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971390
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42520.4377232625413068No Hit
TATCAACGCAGAGTACTTTTTTTTT25890.2665252885041024No Hit
GGTATCAACGCAGAGTACTTTTTTT25090.258289667383852No Hit
GTCCTACAGTGGACATTTCTAAATT19500.20074326480610258No Hit
GTCCTAAAGTGTGTATTTCTCATTT18780.19333120579787727No Hit
CTGTAGGACGTGGAATATGGCAAGA17040.17541872986133272No Hit
CTTTAGGACGTGAAATATGGCGAGG14510.14937357806854096No Hit
GTCCTACAGTGTGCATTTCTCATTT12170.12528438629180863No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11950.12301959048373981No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11120.11447513357148004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT509.04606E-515.1323826
TCCAACG1350.014.71203818
GGTATCA5350.014.3972941
TAGGACC2500.014.3757634
GGCGAGG5100.013.166656519
GCCGGTT1154.0381565E-1013.15859411
CTTATAG802.0996977E-613.0043923
CTAGACT1001.5263322E-712.2950614
TGGACCG701.13640446E-412.159955
TGCCGGT1405.638867E-1112.1599510
CGGTTTC1251.5097612E-912.10590613
GCGTGCA550.003171140812.0371239
CCAACGT951.0958629E-611.94661919
TTAGGAC7300.011.6602263
GTCCTAG1456.384653E-1011.5776651
GTTATAG1052.1746018E-611.285791
CCGGTTT1355.0567905E-911.20917212
ACTGTTC4750.011.1501778
TTTAGGA8150.011.1473162
CCAACGA855.5648707E-511.12675219
Produced by FastQC (version 0.11.5)