FastQCFastQC Report
Thu 26 May 2016
SRR1511685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971390
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62300.641348994739497No Hit
TATCAACGCAGAGTACTTTTTTTTT46860.48240150711866503No Hit
GGTATCAACGCAGAGTACTTTTTTT44990.4631507427500798No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21400.2203028649666972No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16930.17428633195729828No Hit
CTGTAGGACGTGGAATATGGCAAGA16060.16533009398902604No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15280.15730036339678194No Hit
GTCCTAAAGTGTGTATTTCTCATTT14610.15040303070857225No Hit
GTCCTACAGTGGACATTTCTAAATT13390.13784370850019045No Hit
CTTTAGGACGTGAAATATGGCGAGG13380.1377407632361873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10450.014.4141811
CGAACCG500.001501690113.29664912
GCGAACC655.4612807E-513.15053211
CCACCTT3150.012.66347513
TAGGACC3050.012.4590844
GAACAGT3550.012.0392196
TAGGACT1603.6379788E-1211.8750654
TTCCACC3300.011.80005611
AACAGTG3900.011.2023057
TTATGGT600.0058727311.0833944
TTATACC600.0058727311.0833944
AACCGGG600.00588399411.08054214
CGTGCGC600.00588399411.08054210
CGCCACC600.00588399411.08054214
CACCTTT3600.011.08054114
TTTATAC1553.110472E-1011.0323183
CTTACAC951.358547E-511.000063
ACCGGGG700.001493666910.85440715
TCCACCT3700.010.78106712
CTTATAC803.7677403E-410.6875583
Produced by FastQC (version 0.11.5)