##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511685_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 971390 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.534426955187925 31.0 28.0 31.0 23.0 34.0 2 28.724805690814193 31.0 28.0 33.0 23.0 34.0 3 28.710998672006095 31.0 28.0 33.0 22.0 34.0 4 32.21740495578501 35.0 32.0 37.0 25.0 37.0 5 31.655091158031276 35.0 32.0 37.0 20.0 37.0 6 31.657005939941733 35.0 32.0 37.0 19.0 37.0 7 31.366935010654835 35.0 32.0 37.0 17.0 37.0 8 31.212396668691255 35.0 32.0 37.0 17.0 37.0 9 32.292352196337205 37.0 32.0 39.0 17.0 39.0 10 31.94516517567609 37.0 31.0 39.0 11.0 39.0 11 32.015524145811675 37.0 32.0 39.0 11.0 39.0 12 31.69081110573508 37.0 31.0 39.0 10.0 39.0 13 31.68321683360957 37.0 31.0 39.0 10.0 39.0 14 32.16274616786255 37.0 31.0 40.0 10.0 41.0 15 32.045176499655135 37.0 31.0 40.0 10.0 41.0 16 31.819980646290368 37.0 31.0 40.0 8.0 41.0 17 31.747963227951697 37.0 30.0 40.0 2.0 41.0 18 31.601296080873798 37.0 30.0 40.0 2.0 41.0 19 31.63279939056404 37.0 30.0 40.0 2.0 41.0 20 31.71577327335056 37.0 31.0 40.0 2.0 41.0 21 31.606806740855887 37.0 31.0 40.0 2.0 41.0 22 31.39937718115278 37.0 30.0 40.0 2.0 41.0 23 31.179424330083695 37.0 30.0 40.0 2.0 41.0 24 30.84934990065782 37.0 30.0 40.0 2.0 41.0 25 30.16999454390101 36.0 29.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04965765925135912 1101 2 0.2735181124758661 1101 3 0.24431991804159026 1101 4 0.26849413127004595 1101 5 0.340773919362487 1101 6 0.5151932875567553 1101 7 0.24021879528506673 1101 8 0.15215952028028923 1101 9 0.09097835203112226 1101 10 0.4880854700159283 1101 11 0.21103877266883941 1101 12 0.2536131991805597 1101 13 0.4319530228601529 1101 14 -0.040535725490826735 1101 15 -0.23488766142262563 1101 16 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3 0.6672558850281902 1103 4 0.7958257026522517 1103 5 1.098513985203816 1103 6 0.863849104313843 1103 7 0.5925459617894511 1103 8 0.3808635588577438 1103 9 0.6023475735269272 1103 10 0.9481466746340921 1103 11 0.5423310247205855 1103 12 0.7473666187567645 1103 13 0.99031069893935 1103 14 0.9092718508990316 1103 15 0.6452134189316183 1103 16 0.9095701398106542 1103 17 0.781311386516311 1103 18 0.4221801990445506 1103 19 0.9959525195763597 1103 20 0.6766802298727193 1103 21 1.0158785948559732 1103 22 0.9857071097343386 1103 23 0.849187781007025 1103 24 0.886118363121497 1103 25 0.9188615919576222 1104 1 0.42798391319676554 1104 2 0.5501627177941089 1104 3 0.7271454640482062 1104 4 0.8080272209407227 1104 5 0.5013711280609598 1104 6 0.9818477240515904 1104 7 0.7131256719343746 1104 8 0.8557979964008808 1104 9 0.7549355652453542 1104 10 0.7672080963042127 1104 11 0.9632696030504668 1104 12 1.1365591653406142 1104 13 0.8246102158475601 1104 14 0.7318391386837106 1104 15 1.0499201132035338 1104 16 0.9786936732820166 1104 17 0.9791857826515731 1104 18 1.212463152805764 1104 19 0.7130125609842288 1104 20 0.8320701539582949 1104 21 1.034719174566117 1104 22 0.9933951704658774 1104 23 1.2691601757620283 1104 24 1.2463530077039628 1104 25 0.8378678031377476 1105 1 0.11989422008570827 1105 2 0.3490960064484341 1105 3 0.46782290952585015 1105 4 0.323477780824561 1105 5 0.17439617623051618 1105 6 0.25662642333393393 1105 7 0.33343228163530725 1105 8 0.22657233947981936 1105 9 0.33003818734471224 1105 10 0.37221493777750325 1105 11 0.11375409105694345 1105 12 0.03701154030920151 1105 13 0.2905033771667114 1105 14 0.24962417680048787 1105 15 0.43605845058590376 1105 16 0.4263661761442634 1105 17 0.8412728508708334 1105 18 0.5309993567654381 1105 19 0.31418538497685233 1105 20 0.35670225024155755 1105 21 0.7642408299234056 1105 22 0.4153465261945648 1105 23 0.6543295371314386 1105 24 0.8301205651583778 1105 25 0.8737143307409987 1106 1 -0.09538127961452858 1106 2 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-0.3335676212103138 2313 19 -0.09742105520566824 2313 20 -0.3192687976256039 2313 21 -0.11215628106623043 2313 22 -0.17072481514829008 2313 23 -0.03937023374817272 2313 24 0.10719146841995197 2313 25 0.18308694407030046 2314 1 -0.18923172141017375 2314 2 -0.28861637750672386 2314 3 -0.14795335018104439 2314 4 -0.35820027137372534 2314 5 -0.468325841636009 2314 6 -0.1548233343585963 2314 7 -0.2607873715438842 2314 8 -0.5249661669719821 2314 9 -0.3161228449434894 2314 10 -1.7186065721191497 2314 11 -1.104451082061523 2314 12 -0.6947978834077375 2314 13 -0.5985781899495386 2314 14 -0.16504272341554582 2314 15 -0.1926797989615956 2314 16 -0.4953072050666876 2314 17 -0.47303204782712527 2314 18 -0.5447173478529983 2314 19 -0.37512973150589346 2314 20 -0.27552756233507125 2314 21 -0.3225052435278677 2314 22 -0.15284988882266148 2314 23 -0.20870543688619492 2314 24 -0.09431453731140493 2314 25 3.6231345834281115E-4 2315 1 -0.7214133507880653 2315 2 -0.8839245999177514 2315 3 -1.2165269365116416 2315 4 -1.030918103904643 2315 5 -1.1999788200179609 2315 6 -1.0111813066044206 2315 7 -1.1478071015542675 2315 8 -1.4336417074232202 2315 9 -1.2730566691670333 2315 10 -1.7654299460453302 2315 11 -1.463386227912558 2315 12 -1.6714050768373596 2315 13 -1.52152730729118 2315 14 -1.5101763021939831 2315 15 -1.85192647129238 2315 16 -1.6832143135345454 2315 17 -1.7874944286446457 2315 18 -1.4317465180597395 2315 19 -1.3123429855415765 2315 20 -1.4385786337973592 2315 21 -1.4874443542613704 2315 22 -1.3863059447086208 2315 23 -1.4445876232356696 2315 24 -1.630923618780301 2315 25 -1.4913208735519454 2316 1 -0.987469917322791 2316 2 -1.1348461167597215 2316 3 -1.349057414075805 2316 4 -1.4426259232412377 2316 5 -1.4480298585493294 2316 6 -1.5352790543491999 2316 7 -1.5642405075551586 2316 8 -2.3566316591025895 2316 9 -2.07773900655965 2316 10 -1.695350758165663 2316 11 -1.5198375044246895 2316 12 -2.090569553598012 2316 13 -1.8523249599753768 2316 14 -2.0067152627625013 2316 15 -1.689414588719643 2316 16 -1.8503694554777752 2316 17 -1.903593476062973 2316 18 -1.6374352242918029 2316 19 -1.9464475609632572 2316 20 -1.573342925266715 2316 21 -1.7146358519375475 2316 22 -1.6090396938496738 2316 23 -1.706074476115699 2316 24 -1.6421651363247953 2316 25 -1.7447868526210542 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47210.0 3 1.0 4 765.0 5 5579.0 6 6613.0 7 5580.0 8 4507.0 9 4002.0 10 3786.0 11 3573.0 12 3453.0 13 3282.0 14 3170.0 15 3263.0 16 3259.0 17 3337.0 18 3523.0 19 4166.0 20 4978.0 21 6312.0 22 7891.0 23 9672.0 24 11308.0 25 13455.0 26 15238.0 27 17352.0 28 20230.0 29 24081.0 30 29593.0 31 37410.0 32 46363.0 33 58139.0 34 73169.0 35 93364.0 36 120857.0 37 149480.0 38 123429.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.0177799959288 23.457567012098913 15.594566081095461 19.930086910876827 2 17.465178764450943 24.913062724549356 38.73449386960953 18.88726464139017 3 21.07042485510454 28.232120981274257 30.90293291057145 19.794521253049755 4 15.984414087029927 20.890064752571057 37.35986575937574 25.765655401023274 5 12.940693081134071 40.33327703885555 35.415576819307084 11.310453060703296 6 30.090077106002738 40.52769742328004 17.71904178548266 11.66318368523456 7 26.62792493231349 33.89987543623056 23.096181760158125 16.376017871297833 8 24.804455471026053 39.283809798330225 20.51029967366351 15.401435056980203 9 26.988953973172464 15.942618309844656 20.913948053819784 36.154479663163094 10 15.423156507684862 31.346626998424938 34.206240541903874 19.02397595198633 11 34.467618567207815 23.521963372075067 24.945799318502353 17.064618742214765 12 23.25759993411503 26.686603732795273 32.310091724230226 17.74570460885947 13 29.917746734061502 23.305263591348478 26.177745292827804 20.599244381762215 14 21.134971535634502 23.88288946767004 28.637004704598567 26.345134292096894 15 23.07363674734144 32.93157228301712 24.04049866685883 19.954292302782612 16 21.851367627832282 29.791741730921668 28.460762412625208 19.896128228620842 17 21.289904157959214 30.277540431752435 28.337639876877464 20.094915533410884 18 21.14660435046686 28.438835071392543 31.240284540709705 19.1742760374309 19 23.419738724919963 27.78029421756452 29.21411585460011 19.58585120291541 20 23.28426275749184 28.523867859459124 29.65822172350961 18.533647659539422 21 22.969970866490286 27.649450787016544 28.9406932334078 20.439885113085374 22 23.057062559836936 28.753229907658095 29.26877978978577 18.920927742719197 23 22.28991445248561 28.679418153367852 29.379034167533124 19.65163322661341 24 22.88977650583185 28.026436343796 29.881407055868397 19.202380094503752 25 22.59414441310301 28.446128188762383 29.781239061953098 19.178488336181516 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 335.0 1 335.0 2 523.5 3 712.0 4 712.0 5 712.0 6 1627.0 7 2542.0 8 2542.0 9 2542.0 10 3407.0 11 4272.0 12 4272.0 13 4272.0 14 5965.5 15 7659.0 16 7659.0 17 7659.0 18 12841.5 19 18024.0 20 18024.0 21 18024.0 22 27469.0 23 36914.0 24 36914.0 25 36914.0 26 51138.0 27 65362.0 28 65362.0 29 65362.0 30 80256.0 31 95150.0 32 95150.0 33 95150.0 34 101663.0 35 108176.0 36 108176.0 37 108176.0 38 111242.0 39 114308.0 40 114308.0 41 114308.0 42 115688.5 43 117069.0 44 117069.0 45 117069.0 46 115424.5 47 113780.0 48 113780.0 49 113780.0 50 107664.5 51 101549.0 52 101549.0 53 101549.0 54 88086.5 55 74624.0 56 74624.0 57 74624.0 58 62742.0 59 50860.0 60 50860.0 61 50860.0 62 40861.5 63 30863.0 64 30863.0 65 30863.0 66 23826.5 67 16790.0 68 16790.0 69 16790.0 70 12268.0 71 7746.0 72 7746.0 73 7746.0 74 5451.5 75 3157.0 76 3157.0 77 3157.0 78 2112.0 79 1067.0 80 1067.0 81 1067.0 82 692.5 83 318.0 84 318.0 85 318.0 86 205.5 87 93.0 88 93.0 89 93.0 90 56.0 91 19.0 92 19.0 93 19.0 94 10.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3723530198993195 2 0.0 3 0.0 4 0.0 5 0.03150125078495764 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0010294526400312955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 971390.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.40160220048925 #Duplication Level Percentage of deduplicated Percentage of total 1 89.52484162063999 69.2936617818258 2 7.400598101087052 11.456363005320723 3 1.6238594848493788 3.7706797762740907 4 0.5687809277874033 1.7609822044730317 5 0.2706590672446347 1.0474722727412342 6 0.14178570195208323 0.6584664300127367 7 0.09630280097723445 0.5217793764422952 8 0.06361446929542719 0.3939089477279915 9 0.049216376447874875 0.34284837524111456 >10 0.22031838511618324 3.1043697186453576 >50 0.018893783563441135 1.048117600500913 >100 0.01769577405038577 2.7245408353746274 >500 0.0021129273777514236 1.166068465966931 >1k 0.0011885216499851758 2.073941898182769 >5k 1.3205796110946397E-4 0.6367993112705101 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6230 0.641348994739497 No Hit TATCAACGCAGAGTACTTTTTTTTT 4686 0.48240150711866503 No Hit GGTATCAACGCAGAGTACTTTTTTT 4499 0.4631507427500798 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2140 0.2203028649666972 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1693 0.17428633195729828 No Hit CTGTAGGACGTGGAATATGGCAAGA 1606 0.16533009398902604 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1528 0.15730036339678194 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1461 0.15040303070857225 No Hit GTCCTACAGTGGACATTTCTAAATT 1339 0.13784370850019045 No Hit CTTTAGGACGTGAAATATGGCGAGG 1338 0.1377407632361873 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.0589052800625907E-4 0.0 5 0.0 0.0 0.0 2.0589052800625907E-4 0.0 6 0.0 0.0 0.0 3.088357920093886E-4 0.0 7 0.0 0.0 0.0 3.088357920093886E-4 0.0 8 0.0 0.0 0.0 3.088357920093886E-4 0.0 9 0.0 0.0 0.0 3.088357920093886E-4 0.0 10 0.0 0.0 0.0 3.088357920093886E-4 0.0 11 0.0 0.0 0.0 3.088357920093886E-4 0.0 12 0.0 0.0 0.0 3.088357920093886E-4 0.0 13 0.0 0.0 0.0 3.088357920093886E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1045 0.0 14.414181 1 CGAACCG 50 0.0015016901 13.296649 12 GCGAACC 65 5.4612807E-5 13.150532 11 CCACCTT 315 0.0 12.663475 13 TAGGACC 305 0.0 12.459084 4 GAACAGT 355 0.0 12.039219 6 TAGGACT 160 3.6379788E-12 11.875065 4 TTCCACC 330 0.0 11.800056 11 AACAGTG 390 0.0 11.202305 7 TTATGGT 60 0.00587273 11.083394 4 TTATACC 60 0.00587273 11.083394 4 AACCGGG 60 0.005883994 11.080542 14 CGTGCGC 60 0.005883994 11.080542 10 CGCCACC 60 0.005883994 11.080542 14 CACCTTT 360 0.0 11.080541 14 TTTATAC 155 3.110472E-10 11.032318 3 CTTACAC 95 1.358547E-5 11.00006 3 ACCGGGG 70 0.0014936669 10.854407 15 TCCACCT 370 0.0 10.781067 12 CTTATAC 80 3.7677403E-4 10.687558 3 >>END_MODULE