FastQCFastQC Report
Thu 26 May 2016
SRR1511684_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511684_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201548
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42730.355624577628193No Hit
GGTATCAACGCAGAGTACTTTTTTT24960.20773202568686394No Hit
TATCAACGCAGAGTACTTTTTTTTT24950.20764879971503428No Hit
GTCCTACAGTGGACATTTCTAAATT24190.2013236258559791No Hit
CTGTAGGACGTGGAATATGGCAAGA23830.1983274908701109No Hit
GTCCTAAAGTGTGTATTTCTCATTT22200.18476165746187417No Hit
CTTTAGGACGTGAAATATGGCGAGG18920.15746353870174143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATC453.676854E-516.81313113
TTAGTAC400.0054379514.1860793
TCCAACG2000.013.7132118
CCAACGT957.827839E-812.94168819
GGTATCA6900.012.7416221
CCCCGTA604.2489122E-412.60984915
TAGGACC3200.012.1172764
GGCGAGG5400.011.90930219
CCGTACA658.3293155E-411.6398617
GTCCTAG2150.011.4864381
GTCTTAG1301.8337232E-811.3980791
CCAACGA1251.9550498E-811.34886419
TAGGACA3300.011.1769114
AGGGACG600.00607419611.0336188
TCCTAGG1457.836206E-910.9002332
GTCCTAC11700.010.8914991
CTAGCAC1053.6550882E-610.8084413
CTAATAC1159.33318E-710.6909593
AGGACCT7350.010.67976955
TACTGCC803.9291795E-410.6395597
Produced by FastQC (version 0.11.5)