##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511684_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1201548 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.309901060964688 31.0 30.0 34.0 25.0 34.0 2 27.658928315805944 31.0 28.0 34.0 10.0 34.0 3 29.332225595648282 31.0 30.0 34.0 16.0 34.0 4 33.85188440245417 37.0 35.0 37.0 28.0 37.0 5 34.29137412737568 37.0 35.0 37.0 32.0 37.0 6 34.54488459886746 37.0 35.0 37.0 32.0 37.0 7 34.62216906856821 37.0 35.0 37.0 32.0 37.0 8 34.64959452306525 37.0 35.0 37.0 32.0 37.0 9 36.35676394118254 39.0 37.0 39.0 32.0 39.0 10 36.26389707277612 39.0 37.0 39.0 32.0 39.0 11 36.28296081388342 39.0 37.0 39.0 32.0 39.0 12 36.21141061364173 39.0 37.0 39.0 32.0 39.0 13 36.2055864601331 39.0 37.0 39.0 32.0 39.0 14 37.49658357385639 40.0 38.0 41.0 32.0 41.0 15 37.456198171026045 40.0 38.0 41.0 32.0 41.0 16 37.408399830884825 40.0 38.0 41.0 32.0 41.0 17 37.396195574375724 40.0 38.0 41.0 32.0 41.0 18 37.354975415047925 40.0 38.0 41.0 32.0 41.0 19 37.3819164943889 40.0 38.0 41.0 32.0 41.0 20 37.333687875973325 40.0 38.0 41.0 32.0 41.0 21 37.27275065165936 40.0 38.0 41.0 32.0 41.0 22 37.18514449693229 40.0 38.0 41.0 32.0 41.0 23 37.102964675568515 40.0 38.0 41.0 32.0 41.0 24 37.038735864068684 40.0 38.0 41.0 32.0 41.0 25 36.949997003865015 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.048432825062367 1101 2 3.6350644745232543 1101 3 2.1403754758317 1101 4 1.079836990683198 1101 5 0.6424845121164182 1101 6 0.38496743377021403 1101 7 0.235873957701358 1101 8 0.2276164137287111 1101 9 0.17865947288994732 1101 10 0.2729255944934792 1101 11 0.20754457938688375 1101 12 0.27272055572908016 1101 13 0.25801507802929535 1101 14 0.39675116734701277 1101 15 0.23049354605812056 1101 16 0.24183210003743483 1101 17 0.4093270644589353 1101 18 0.2847866894317761 1101 19 0.3341827744762469 1101 20 0.46200525374312207 1101 21 0.45059722983832984 1101 22 0.2995531519096559 1101 23 0.35751467972026063 1101 24 0.5422424735821352 1101 25 0.3531466893244115 1102 1 1.6321188307133205 1102 2 4.391084289748374 1102 3 2.8434747708149395 1102 4 1.7884814994461138 1102 5 1.3210852739378112 1102 6 1.0186156926222694 1102 7 0.935180744502027 1102 8 0.9525492195085192 1102 9 1.097422091017755 1102 10 1.0739802916952996 1102 11 1.0888450365288946 1102 12 1.0054953511955347 1102 13 1.0125225622235874 1102 14 1.070133772343361 1102 15 1.227668069059078 1102 16 1.2010169904979477 1102 17 1.2493322776976115 1102 18 1.1164249005040077 1102 19 1.183936375176586 1102 20 1.192897627414304 1102 21 1.2545634716588339 1102 22 1.020677465520393 1102 23 1.1409642453246889 1102 24 1.135403294618044 1102 25 1.009257367365123 1103 1 1.1839041764305556 1103 2 3.8217654042994553 1103 3 2.352484058740636 1103 4 1.3474804674543677 1103 5 0.9580408982928148 1103 6 0.7028468587729364 1103 7 0.5822808477011989 1103 8 0.4811353908191549 1103 9 0.5549556307494399 1103 10 0.38409381078714233 1103 11 0.538958555620745 1103 12 0.622213617449404 1103 13 0.5565576499428886 1103 14 0.690825206811887 1103 15 0.6112800401633081 1103 16 0.7061047097961932 1103 17 0.7187646822383726 1103 18 0.7373743329085514 1103 19 0.6971561894180738 1103 20 0.701648917403979 1103 21 0.7350794675308663 1103 22 0.9894184355926328 1103 23 0.7746381359335643 1103 24 0.8582928715014333 1103 25 0.6209807935777079 1104 1 1.579938798192373 1104 2 4.174014754000545 1104 3 2.597956609281411 1104 4 1.508335971797301 1104 5 1.1533471852761892 1104 6 0.9826637102613986 1104 7 0.7719515150371805 1104 8 0.8313354242848234 1104 9 0.9316330977020826 1104 10 0.9319725433507671 1104 11 1.0617234288314705 1104 12 0.9361277323583579 1104 13 0.844673988798057 1104 14 1.1071047364670363 1104 15 1.073253602280161 1104 16 0.8540521991099936 1104 17 1.041308681994991 1104 18 1.022833497179377 1104 19 1.0510045899109315 1104 20 1.2033924790644903 1104 21 1.1517922255588218 1104 22 1.3663368554043132 1104 23 1.334675860876139 1104 24 1.2513642598705914 1104 25 1.1456532102557873 1105 1 1.429108228616883 1105 2 3.9519408269084693 1105 3 2.4625109416859132 1105 4 1.4861041214085944 1105 5 1.1085331447653743 1105 6 0.7882149424897733 1105 7 0.813207473967509 1105 8 0.7988583901660604 1105 9 0.9118548272987042 1105 10 0.727208283532093 1105 11 0.7103374836443166 1105 12 0.9398059990960945 1105 13 0.7741138839004549 1105 14 0.8025519424325509 1105 15 0.8106682633069369 1105 16 0.931913552310732 1105 17 0.9206097331944676 1105 18 0.9517878442464678 1105 19 0.8737507125875297 1105 20 0.977180577008177 1105 21 1.0837728348855507 1105 22 0.937939266078196 1105 23 1.0117864173995201 1105 24 0.9000492239262101 1105 25 1.023926678360752 1106 1 1.131578722884651 1106 2 3.720933445347651 1106 3 2.2409868991291297 1106 4 1.2558073870072377 1106 5 0.8247936928685888 1106 6 0.7175417460736568 1106 7 0.5903677086137904 1106 8 0.4943569411215165 1106 9 0.37348229375108843 1106 10 0.4482316154417134 1106 11 0.5723138790585338 1106 12 0.5721311545577024 1106 13 0.6376901403222206 1106 14 0.6763758756775644 1106 15 0.7901493822926824 1106 16 0.6711880889422446 1106 17 0.6044362966785428 1106 18 0.6870078311676622 1106 19 0.6577700798340516 1106 20 0.5005878948641254 1106 21 0.5715995335011783 1106 22 0.6324219173591672 1106 23 0.7226242088674084 1106 24 0.5756393988974366 1106 25 0.5275631648439756 1107 1 1.3005671435041783 1107 2 3.885043243737119 1107 3 2.3588159122367536 1107 4 1.357362080476939 1107 5 0.9530852934093517 1107 6 0.888153924995656 1107 7 0.7305031932431874 1107 8 0.5977558125727569 1107 9 0.719004835336932 1107 10 0.6725233335396439 1107 11 0.5942970683843427 1107 12 0.7664237543343475 1107 13 0.8004847679599081 1107 14 0.9349428538707087 1107 15 0.9737883642476532 1107 16 0.9854110361002952 1107 17 0.8620554702516969 1107 18 0.8994997491127634 1107 19 0.9763940732508516 1107 20 1.0814084438560698 1107 21 0.9001497597987154 1107 22 0.9764162158368066 1107 23 1.0667728011203934 1107 24 1.0734205074614778 1107 25 1.0161454254682027 1108 1 1.118806137214868 1108 2 3.778469419238764 1108 3 2.3725400811496016 1108 4 1.1846606728698674 1108 5 0.8044554641191439 1108 6 0.5707460184368074 1108 7 0.43521268710362904 1108 8 0.45074323395640903 1108 9 0.4393627269212388 1108 10 0.3979126716648267 1108 11 0.442889461316355 1108 12 0.3382266893448289 1108 13 0.4227294519970428 1108 14 0.3555777745056332 1108 15 0.4998141204620907 1108 16 0.46673029336295 1108 17 0.46347056459131863 1108 18 0.3708010369234884 1108 19 0.33600773003431783 1108 20 0.3407525588605651 1108 21 0.5389263396130275 1108 22 0.5333119930156016 1108 23 0.6484289856067633 1108 24 0.5886196688870555 1108 25 0.6513356021678121 1109 1 0.8593554102358709 1109 2 2.8882996625908177 1109 3 1.7259364962584556 1109 4 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-1.5958937130271948 2315 11 -1.5106986864678333 2315 12 -1.669344725578263 2315 13 -1.4374713150394953 2315 14 -1.982153735409888 2315 15 -1.7445852844704177 2315 16 -1.8937529942484517 2315 17 -1.8745919174952448 2315 18 -1.9708414039450943 2315 19 -1.8435873238083715 2315 20 -1.8916267557199546 2315 21 -2.121448443719622 2315 22 -2.019853056323136 2315 23 -1.9829792497997474 2315 24 -1.8264467302722096 2315 25 -1.7534147015076869 2316 1 -0.229592439297587 2316 2 2.2532307591246585 2316 3 0.8341482831492613 2316 4 -0.14659391674771172 2316 5 -0.3025857153419622 2316 6 -0.5518094806311495 2316 7 -1.040387493656695 2316 8 -1.0311666118324396 2316 9 -1.167090981489764 2316 10 -1.0983393760107063 2316 11 -1.1431355062032722 2316 12 -1.3995155012443377 2316 13 -1.3958239005420765 2316 14 -1.5502291508742445 2316 15 -1.4487143775325961 2316 16 -1.50477171194688 2316 17 -1.49352824916906 2316 18 -1.5835767521518136 2316 19 -1.5887931511722542 2316 20 -1.4961056007733973 2316 21 -1.8125536355324243 2316 22 -1.62960954570665 2316 23 -1.6847659531041899 2316 24 -1.8837014066368667 2316 25 -1.713520636887715 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29588.0 3 3.0 4 248.0 5 1249.0 6 1515.0 7 1317.0 8 1220.0 9 1148.0 10 1083.0 11 1046.0 12 1012.0 13 978.0 14 1011.0 15 1054.0 16 1081.0 17 1219.0 18 1292.0 19 1557.0 20 2010.0 21 2523.0 22 3107.0 23 3686.0 24 4266.0 25 4984.0 26 5661.0 27 6617.0 28 7893.0 29 9746.0 30 12408.0 31 16978.0 32 23141.0 33 33800.0 34 50960.0 35 81001.0 36 145134.0 37 197048.0 38 542964.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50625027048441 18.700542966240217 13.17808360548226 25.615123157793114 2 15.380175167887336 20.85002064633908 40.918088366331254 22.85171581944233 3 17.792131483719338 25.182181652335156 30.33794738121157 26.687739482733942 4 11.610938555929518 17.316162150825434 39.72791765289443 31.344981640350618 5 12.848841660924073 37.3608877880867 36.78754406815209 13.00272648283714 6 29.920402680542104 38.16426809415854 19.09370245716359 12.821626768135772 7 26.25712830448721 31.8394271389907 23.63767406712008 18.265770489402005 8 23.239521017886926 37.89469917140222 21.786395549740835 17.079384260970016 9 25.067995618984845 15.731456421216631 21.34163595628306 37.858912003515464 10 14.157570067945683 29.12576110151238 35.59924364236801 21.11742518817392 11 32.97995585694454 22.642208218065363 25.91606827192921 18.461767653060885 12 22.279592658803477 24.540010053697397 33.430041912599414 19.750355374899716 13 28.020686647557984 22.22041899283258 26.88598374763222 22.872910611977215 14 19.82667359106752 22.529437026236153 28.88415610529084 28.75973327740548 15 21.877944118753476 31.327920316125528 24.58728240569665 22.20685315942434 16 20.84744013555846 28.233661909470115 29.342231854241362 21.57666610073006 17 20.493147173479546 28.454710090649726 29.35163638905812 21.700506346812613 18 20.17197814818884 27.68828211607027 31.505940669869204 20.633799065871692 19 22.18964385080234 26.892081624772064 28.925460260813768 21.992814263611827 20 21.915728709964146 27.693691804239194 29.770512705276857 20.620066780519796 21 21.592015619876392 27.009952178276986 28.70010802749125 22.69792417435537 22 21.52385454569666 28.397079728213647 29.06605899801254 21.013006728077155 23 20.96118714810638 27.907801270874337 29.285039553571057 21.845972027448223 24 21.74727934298089 26.938499335856747 30.05739263017374 21.256828690988623 25 21.381334744845816 27.47422491652435 29.787906933389262 21.356533405240576 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 301.0 1 301.0 2 396.5 3 492.0 4 492.0 5 492.0 6 1198.5 7 1905.0 8 1905.0 9 1905.0 10 2663.0 11 3421.0 12 3421.0 13 3421.0 14 5304.0 15 7187.0 16 7187.0 17 7187.0 18 12368.0 19 17549.0 20 17549.0 21 17549.0 22 27148.5 23 36748.0 24 36748.0 25 36748.0 26 52454.0 27 68160.0 28 68160.0 29 68160.0 30 87635.0 31 107110.0 32 107110.0 33 107110.0 34 117235.0 35 127360.0 36 127360.0 37 127360.0 38 133860.0 39 140360.0 40 140360.0 41 140360.0 42 145425.5 43 150491.0 44 150491.0 45 150491.0 46 151068.0 47 151645.0 48 151645.0 49 151645.0 50 143259.0 51 134873.0 52 134873.0 53 134873.0 54 118231.0 55 101589.0 56 101589.0 57 101589.0 58 85833.0 59 70077.0 60 70077.0 61 70077.0 62 56667.5 63 43258.0 64 43258.0 65 43258.0 66 33064.5 67 22871.0 68 22871.0 69 22871.0 70 16614.0 71 10357.0 72 10357.0 73 10357.0 74 7123.0 75 3889.0 76 3889.0 77 3889.0 78 2635.5 79 1382.0 80 1382.0 81 1382.0 82 883.0 83 384.0 84 384.0 85 384.0 86 239.0 87 94.0 88 94.0 89 94.0 90 63.0 91 32.0 92 32.0 93 32.0 94 21.0 95 10.0 96 10.0 97 10.0 98 6.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.263083955031343 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 8.322597182967305E-5 20 0.0 21 1.664519436593461E-4 22 0.0013316155492747689 23 0.0010819376337857496 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1201548.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.00717493418526 #Duplication Level Percentage of deduplicated Percentage of total 1 88.55903981709129 69.08240511015318 2 8.455915431080594 13.19244148521961 3 1.645155005551601 3.8500168293574277 4 0.5520827070313501 1.7226564922213232 5 0.22529581056788742 0.8787344853454129 6 0.13309857709124295 0.622958639198864 7 0.08697658409148906 0.47493583272818635 8 0.052422364420094486 0.3271456441425536 9 0.04160055860744163 0.29206278473854724 >10 0.21199970853161856 3.0217808478624724 >50 0.016452739197118982 0.9171856628995492 >100 0.016741250253229624 2.613707828044196 >500 0.002146297656739093 1.212084992106982 >1k 0.0010731488283695465 1.7918833659817632 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4273 0.355624577628193 No Hit GGTATCAACGCAGAGTACTTTTTTT 2496 0.20773202568686394 No Hit TATCAACGCAGAGTACTTTTTTTTT 2495 0.20764879971503428 No Hit GTCCTACAGTGGACATTTCTAAATT 2419 0.2013236258559791 No Hit CTGTAGGACGTGGAATATGGCAAGA 2383 0.1983274908701109 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2220 0.18476165746187417 No Hit CTTTAGGACGTGAAATATGGCGAGG 1892 0.15746353870174143 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 8.322597182967305E-5 0.0 4 0.0 0.0 0.0 8.322597182967305E-5 0.0 5 0.0 0.0 0.0 8.322597182967305E-5 0.0 6 0.0 0.0 0.0 8.322597182967305E-5 0.0 7 0.0 0.0 0.0 8.322597182967305E-5 0.0 8 0.0 0.0 0.0 8.322597182967305E-5 0.0 9 0.0 0.0 0.0 8.322597182967305E-5 0.0 10 0.0 0.0 0.0 8.322597182967305E-5 0.0 11 0.0 0.0 0.0 8.322597182967305E-5 0.0 12 0.0 0.0 0.0 8.322597182967305E-5 0.0 13 0.0 0.0 0.0 8.322597182967305E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAAATC 45 3.676854E-5 16.813131 13 TTAGTAC 40 0.00543795 14.186079 3 TCCAACG 200 0.0 13.71321 18 CCAACGT 95 7.827839E-8 12.941688 19 GGTATCA 690 0.0 12.741622 1 CCCCGTA 60 4.2489122E-4 12.609849 15 TAGGACC 320 0.0 12.117276 4 GGCGAGG 540 0.0 11.909302 19 CCGTACA 65 8.3293155E-4 11.63986 17 GTCCTAG 215 0.0 11.486438 1 GTCTTAG 130 1.8337232E-8 11.398079 1 CCAACGA 125 1.9550498E-8 11.348864 19 TAGGACA 330 0.0 11.176911 4 AGGGACG 60 0.006074196 11.033618 8 TCCTAGG 145 7.836206E-9 10.900233 2 GTCCTAC 1170 0.0 10.891499 1 CTAGCAC 105 3.6550882E-6 10.808441 3 CTAATAC 115 9.33318E-7 10.690959 3 AGGACCT 735 0.0 10.6797695 5 TACTGCC 80 3.9291795E-4 10.639559 7 >>END_MODULE