FastQCFastQC Report
Thu 26 May 2016
SRR1511684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201548
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62790.522575877118517No Hit
TATCAACGCAGAGTACTTTTTTTTT43520.36219942940273714No Hit
GGTATCAACGCAGAGTACTTTTTTT41100.34205874421995625No Hit
CTGTAGGACGTGGAATATGGCAAGA22610.18817392230689078No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20500.17061324225082977No Hit
CTTTAGGACGTGAAATATGGCGAGG19010.15821257244820847No Hit
GTCCTACAGTGGACATTTCTAAATT18290.15222030247647203No Hit
GTCCTAAAGTGTGTATTTCTCATTT18070.15038933109621921No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16260.1353254301950484No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14290.11892991374460278No Hit
CTGAAGGACCTGGAATATGGCGAGA12430.10344988298428362No Hit
TTGTAGAACAGTGTATATCAATGAG12160.10120278174488243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCA400.005286547414.24659819
GGTATCA11350.013.0143391
AGCGTAC603.9839445E-412.7064731
GCCGGTT1001.4458419E-712.34705111
GCGTGCG550.003073239712.0880229
CGGTTTC1201.00371835E-811.87216513
ACTGTTC5850.011.6895168
CACCTTT3300.011.51240314
TCCAACG1657.2759576E-1211.51240318
TAGGACC3400.011.4564854
GGCGAGG5650.011.43089919
TGTCGAG752.0776695E-411.39727918
TGCCGGT1104.979338E-711.22459210
TAACACC600.005872043311.0839174
GCGTACA700.001490321610.8577142
CCAACGT700.001493948510.85455119
TGTAGAA6150.010.8135772
CCGGTTT1158.835432E-710.73656712
AGGACGT13800.010.6708515
GGACGTG13500.010.6233886
Produced by FastQC (version 0.11.5)