##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511684_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1201548 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.805744755931514 31.0 29.0 33.0 23.0 34.0 2 29.002852154054604 31.0 29.0 33.0 24.0 34.0 3 29.011436080789114 31.0 28.0 34.0 24.0 34.0 4 32.533619963580314 35.0 32.0 37.0 26.0 37.0 5 31.996787477487374 35.0 32.0 37.0 22.0 37.0 6 31.98689939977429 35.0 32.0 37.0 22.0 37.0 7 31.710520928002875 35.0 32.0 37.0 19.0 37.0 8 31.55867431013992 35.0 32.0 37.0 18.0 37.0 9 32.697838954415474 37.0 32.0 39.0 17.0 39.0 10 32.348437182700984 37.0 32.0 39.0 17.0 39.0 11 32.414219823094875 37.0 32.0 39.0 17.0 39.0 12 32.10108959442319 37.0 32.0 39.0 11.0 39.0 13 32.08874967958001 37.0 32.0 39.0 11.0 39.0 14 32.63426180227506 37.0 32.0 40.0 10.0 41.0 15 32.52289213581147 37.0 31.0 40.0 10.0 41.0 16 32.29345394441171 37.0 31.0 40.0 10.0 41.0 17 32.22885810637611 37.0 31.0 40.0 10.0 41.0 18 32.08216900198744 37.0 31.0 40.0 8.0 41.0 19 32.11120071774078 37.0 31.0 40.0 2.0 41.0 20 32.18419155955484 37.0 31.0 40.0 2.0 41.0 21 32.08285062269672 37.0 31.0 40.0 2.0 41.0 22 31.894273054426456 37.0 31.0 40.0 2.0 41.0 23 31.694593141514112 37.0 31.0 40.0 2.0 41.0 24 31.383147406512265 37.0 31.0 40.0 2.0 41.0 25 30.71455572311718 36.0 30.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.12249112683936758 1101 2 -0.015322662941734677 1101 3 0.03724079592198137 1101 4 -0.18422512285135184 1101 5 -0.08622030609438269 1101 6 -0.4922676959817309 1101 7 -0.19500815583372955 1101 8 -0.25216117413402017 1101 9 -0.5066573696535315 1101 10 -0.24974461736625386 1101 11 -0.4317293168545646 1101 12 -0.3505431691384935 1101 13 -0.5197003393165751 1101 14 -0.7078908396447154 1101 15 -0.5231509895927715 1101 16 -1.1044160656785742 1101 17 -0.8725738258039542 1101 18 -0.7604358368476412 1101 19 -0.9272568040402405 1101 20 -1.184809216436232 1101 21 -1.1170794031322089 1101 22 -1.1023379365929458 1101 23 -0.9308116468027698 1101 24 -1.5669608656434626 1101 25 -1.0585629203266151 1102 1 0.2812563802735042 1102 2 0.3208340981616047 1102 3 0.3760097092813979 1102 4 0.4570640963186108 1102 5 0.64460321348108 1102 6 0.4779639734595911 1102 7 0.7116342971426128 1102 8 0.4610424593949709 1102 9 0.6729479242427985 1102 10 0.8190689036108836 1102 11 0.6094921548033696 1102 12 0.8861495473277188 1102 13 0.6757260273757524 1102 14 0.8642436619131075 1102 15 0.713116921693647 1102 16 0.38083255908382085 1102 17 0.6500447446374764 1102 18 0.7027880840488407 1102 19 0.7795366361316951 1102 20 0.9991547876261606 1102 21 0.7306639038808314 1102 22 0.5702380918536285 1102 23 0.8609157795049036 1102 24 0.4426504283954955 1102 25 0.5728543428706274 1103 1 0.28312428533026335 1103 2 0.42087537783136497 1103 3 0.37086007047851055 1103 4 0.49612498383151404 1103 5 0.7581429451632324 1103 6 0.4575511767619638 1103 7 0.41906463769988633 1103 8 0.5944067524806265 1103 9 0.5797074701664329 1103 10 0.5953537333322458 1103 11 0.19698441486529106 1103 12 0.5006490313318466 1103 13 0.7254061099350864 1103 14 0.6980310716138334 1103 15 0.7706607813427695 1103 16 0.939934726266479 1103 17 1.2154730212112597 1103 18 0.8022204885896045 1103 19 0.942281734170912 1103 20 0.7660381725590781 1103 21 0.8300756685867086 1103 22 0.9783392270445468 1103 23 1.0529694430755931 1103 24 1.0233109031116996 1103 25 1.0772815874526707 1104 1 0.5335789621583267 1104 2 0.5814856700475524 1104 3 0.5964406238790794 1104 4 0.9357027078613882 1104 5 0.8237338424996672 1104 6 1.043058714144646 1104 7 0.966274056766192 1104 8 1.0366878945728395 1104 9 1.3347630688217222 1104 10 1.188725704308382 1104 11 1.3690028514098032 1104 12 1.4710288667415696 1104 13 1.1413412473725302 1104 14 1.3215172822346162 1104 15 1.4172243128841657 1104 16 1.3838702896306927 1104 17 1.645744245294381 1104 18 1.6145449839424373 1104 19 1.3618165612902757 1104 20 1.3276962446319729 1104 21 1.5347185746242218 1104 22 1.222909421297583 1104 23 1.5549150157189295 1104 24 1.5018858738292309 1104 25 1.4958422592000353 1105 1 0.45792661214790087 1105 2 0.40629236724525697 1105 3 0.49559025379893384 1105 4 0.5538792706740523 1105 5 0.6551633427878123 1105 6 0.5666309294996204 1105 7 0.5539350072264746 1105 8 0.6825779527965388 1105 9 0.6360462256531392 1105 10 0.6464604905200204 1105 11 0.557596799245843 1105 12 0.8667613321778234 1105 13 0.9295804086243535 1105 14 0.9273538340263059 1105 15 1.0525422837500855 1105 16 0.8778415016799741 1105 17 1.0325535637303602 1105 18 1.0542610104528833 1105 19 1.0482066138051849 1105 20 1.1255400027256925 1105 21 1.0976730623079476 1105 22 1.1863737344183711 1105 23 1.486416687194005 1105 24 1.0939167566072427 1105 25 1.4529812274945861 1106 1 -0.18396935586453012 1106 2 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1.0354116593246587 1107 14 0.9558353631417589 1107 15 1.003182054002778 1107 16 0.9859983592668513 1107 17 1.1471970315701228 1107 18 0.8215231153006641 1107 19 0.7772243904787217 1107 20 0.9529804589075823 1107 21 0.7504567266703219 1107 22 1.1996581319650055 1107 23 1.2559106195461816 1107 24 1.1200848579766642 1107 25 0.9414676769828212 1108 1 0.19998703042830357 1108 2 0.21166902862501757 1108 3 -0.003266913648474201 1108 4 0.40200733677268374 1108 5 0.05512707214735357 1108 6 0.3954316622895142 1108 7 0.2633223407811194 1108 8 0.3523712716216174 1108 9 0.11621540667437102 1108 10 0.38589528328556355 1108 11 0.2944914176664071 1108 12 0.252049591555938 1108 13 0.19813253383798468 1108 14 0.28345590821513866 1108 15 0.3522760941345524 1108 16 0.3938749690302501 1108 17 0.42163548619725333 1108 18 0.4480654932581345 1108 19 0.30675418981984137 1108 20 0.284254792540402 1108 21 0.15596735859604394 1108 22 0.15579020743670569 1108 23 0.25908522272078827 1108 24 0.36655086576342555 1108 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2313 16 -0.6816792747058074 2313 17 -0.7192539274194303 2313 18 -0.48099130852593674 2313 19 -0.6072888378595529 2313 20 -0.5195298066987419 2313 21 -0.5508868982582094 2313 22 -0.4063310046845068 2313 23 -0.49050811323618504 2313 24 -0.642179292966734 2313 25 -0.5783917231939029 2314 1 -0.15762886361143202 2314 2 -0.12168532857040049 2314 3 -0.2888971039144259 2314 4 -0.2962266588123015 2314 5 -0.27411974799350247 2314 6 -0.2758444861389542 2314 7 -0.44195990456735146 2314 8 -0.5843258796450677 2314 9 -0.4990452892154664 2314 10 -1.4999267497219009 2314 11 -0.5310210389718755 2314 12 -0.3518766146881518 2314 13 -0.4948165884157767 2314 14 -0.3154587349048015 2314 15 -0.22178046005445395 2314 16 0.09493667406546535 2314 17 -0.11420494268953263 2314 18 -0.3169718240970596 2314 19 -0.3374773880210107 2314 20 -0.23866121713057353 2314 21 -0.3207092944051091 2314 22 -0.24912149234254244 2314 23 -0.19717988818008436 2314 24 -0.016020352569380947 2314 25 0.011165498633033621 2315 1 -1.0063758242578515 2315 2 -1.1983966198757123 2315 3 -1.1615446300087378 2315 4 -1.4539399260533372 2315 5 -1.4932528702566614 2315 6 -1.4261579595150735 2315 7 -1.7510329800230409 2315 8 -1.6616578539294338 2315 9 -1.8013186118054776 2315 10 -1.8800132182635245 2315 11 -1.831265041409054 2315 12 -1.749184900521204 2315 13 -1.6358347647466829 2315 14 -1.8895783900556182 2315 15 -1.8745353813871581 2315 16 -1.8639032181976347 2315 17 -1.8412396724646634 2315 18 -1.630875122827792 2315 19 -1.750102734663912 2315 20 -1.7144588517020445 2315 21 -1.8064485335274973 2315 22 -2.069818849599695 2315 23 -1.8645393661230614 2315 24 -1.9664269283923552 2315 25 -2.2281334143176004 2316 1 -0.983286990924011 2316 2 -1.1374638867000684 2316 3 -1.1261387432818708 2316 4 -1.2448491282332448 2316 5 -1.4146803051333308 2316 6 -1.6058449574924474 2316 7 -1.7786535955008667 2316 8 -2.598219023808536 2316 9 -2.28927236376002 2316 10 -1.9199822515955312 2316 11 -1.779724816870111 2316 12 -1.880817719069384 2316 13 -1.8029492037206225 2316 14 -2.001557931247099 2316 15 -1.7554881266189923 2316 16 -1.9356482756175453 2316 17 -1.9775595654888996 2316 18 -1.9161976259871878 2316 19 -1.9835468890933399 2316 20 -1.947076055338485 2316 21 -1.7872343586270212 2316 22 -1.8893462101196405 2316 23 -1.8386919901809797 2316 24 -1.8991187947460944 2316 25 -2.073088937981705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52942.0 3 1.0 4 903.0 5 6312.0 6 7720.0 7 6300.0 8 5292.0 9 4499.0 10 4236.0 11 4290.0 12 3986.0 13 3733.0 14 3641.0 15 3525.0 16 3776.0 17 3839.0 18 4159.0 19 4686.0 20 5767.0 21 7326.0 22 9163.0 23 11177.0 24 13443.0 25 15575.0 26 17411.0 27 20201.0 28 23764.0 29 28083.0 30 34999.0 31 44180.0 32 55125.0 33 70166.0 34 89564.0 35 116035.0 36 152377.0 37 196003.0 38 167349.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.488776321680845 23.08931829269108 15.614328483884549 20.80757690174353 2 17.027451254548296 24.473013146374512 38.97971616614567 19.519819432931516 3 20.84469367848808 27.605056144240596 30.825984480020775 20.724265697250548 4 15.555267038853213 20.658683631448767 37.373205231917495 26.41284409778053 5 13.058590036247267 39.65471022998783 35.733694973501365 11.553004760263539 6 30.26362658836767 40.128817159197965 17.866452276563233 11.741103975871127 7 26.793602918901282 33.32892235682636 23.0863020037485 16.791172720523857 8 24.57970884226015 39.1245293571293 20.744489608405157 15.551272192205387 9 26.648040694171186 15.916883886453142 20.84302915905149 36.59204626032418 10 15.136557174578128 31.11452892435425 34.4307509978794 19.31816290318822 11 34.48984143787847 23.230199709042 25.028712960281236 17.251245892798288 12 23.10402913574822 26.19895335017827 32.47460775599477 18.222409758078744 13 29.536980628322794 23.12125691191696 26.22741663254402 21.114345827216223 14 20.941402257754163 23.636675355458124 28.48666886383232 26.93525352295539 15 22.94814689051124 32.61767320156997 23.93645530598861 20.497724601930177 16 21.792138141797082 29.725071324657858 28.398615785636526 20.08417474790853 17 21.33622626811413 29.81395666257195 28.461035264508784 20.388781804805134 18 21.043354073245514 28.580048404225217 30.944331811962567 19.4322657105667 19 23.34105670351913 27.754113859787537 28.744669376504312 20.16016006018902 20 23.168862167803532 28.439646189748558 29.336572488156943 19.054919154290964 21 22.751733596993212 27.68553565899989 28.600105863436166 20.96262488057073 22 22.705543182627743 28.884905139037308 28.95306720996581 19.456484468369137 23 22.31912499542257 28.60460006591497 28.898221294530057 20.1780536441324 24 22.750568433387595 28.111236504908664 29.471065658633695 19.66712940307004 25 22.67435134516552 28.407482158090758 29.31782526372735 19.600341233016376 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 294.0 1 294.0 2 464.0 3 634.0 4 634.0 5 634.0 6 1583.5 7 2533.0 8 2533.0 9 2533.0 10 3446.5 11 4360.0 12 4360.0 13 4360.0 14 6276.5 15 8193.0 16 8193.0 17 8193.0 18 14294.0 19 20395.0 20 20395.0 21 20395.0 22 31288.0 23 42181.0 24 42181.0 25 42181.0 26 59433.5 27 76686.0 28 76686.0 29 76686.0 30 96347.0 31 116008.0 32 116008.0 33 116008.0 34 124763.5 35 133519.0 36 133519.0 37 133519.0 38 138169.5 39 142820.0 40 142820.0 41 142820.0 42 145941.5 43 149063.0 44 149063.0 45 149063.0 46 146980.0 47 144897.0 48 144897.0 49 144897.0 50 136355.5 51 127814.0 52 127814.0 53 127814.0 54 111133.5 55 94453.0 56 94453.0 57 94453.0 58 79002.5 59 63552.0 60 63552.0 61 63552.0 62 51112.5 63 38673.0 64 38673.0 65 38673.0 66 29625.0 67 20577.0 68 20577.0 69 20577.0 70 14946.5 71 9316.0 72 9316.0 73 9316.0 74 6531.0 75 3746.0 76 3746.0 77 3746.0 78 2532.5 79 1319.0 80 1319.0 81 1319.0 82 845.0 83 371.0 84 371.0 85 371.0 86 244.5 87 118.0 88 118.0 89 118.0 90 70.5 91 23.0 92 23.0 93 23.0 94 13.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3691904110364297 2 0.0 3 0.0 4 0.0 5 0.02946199402770426 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0019141973520824802 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1201548.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.46016986766196 #Duplication Level Percentage of deduplicated Percentage of total 1 89.87902948710278 70.51923921098683 2 7.452603463907935 11.694650674690852 3 1.427530121412979 3.360127674517993 4 0.4781973670584603 1.5007778659870197 5 0.2201106213078707 0.8634958368746077 6 0.13090404327866215 0.6162452083204564 7 0.07647036475971186 0.4199914466222344 8 0.049479922729754336 0.3105762513932251 9 0.038064709557444426 0.2687907220056275 >10 0.2077544189696243 2.8966620363586957 >50 0.019423788207294228 1.0705719799457445 >100 0.016640265273183654 2.6432490901409125 >500 0.002422300221935107 1.2651817931623157 >1k 0.0012638088114444036 2.051592551801579 >5k 1.053174009537003E-4 0.5188476571919654 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6279 0.522575877118517 No Hit TATCAACGCAGAGTACTTTTTTTTT 4352 0.36219942940273714 No Hit GGTATCAACGCAGAGTACTTTTTTT 4110 0.34205874421995625 No Hit CTGTAGGACGTGGAATATGGCAAGA 2261 0.18817392230689078 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2050 0.17061324225082977 No Hit CTTTAGGACGTGAAATATGGCGAGG 1901 0.15821257244820847 No Hit GTCCTACAGTGGACATTTCTAAATT 1829 0.15222030247647203 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1807 0.15038933109621921 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1626 0.1353254301950484 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1429 0.11892991374460278 No Hit CTGAAGGACCTGGAATATGGCGAGA 1243 0.10344988298428362 No Hit TTGTAGAACAGTGTATATCAATGAG 1216 0.10120278174488243 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 8.322597182967305E-5 0.0 0.0 0.0 6 0.0 8.322597182967305E-5 0.0 0.0 0.0 7 0.0 8.322597182967305E-5 0.0 0.0 0.0 8 0.0 8.322597182967305E-5 0.0 0.0 0.0 9 0.0 8.322597182967305E-5 0.0 0.0 0.0 10 0.0 8.322597182967305E-5 0.0 0.0 0.0 11 0.0 8.322597182967305E-5 0.0 0.0 0.0 12 0.0 8.322597182967305E-5 0.0 0.0 0.0 13 0.0 8.322597182967305E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGCCA 40 0.0052865474 14.246598 19 GGTATCA 1135 0.0 13.014339 1 AGCGTAC 60 3.9839445E-4 12.706473 1 GCCGGTT 100 1.4458419E-7 12.347051 11 GCGTGCG 55 0.0030732397 12.088022 9 CGGTTTC 120 1.00371835E-8 11.872165 13 ACTGTTC 585 0.0 11.689516 8 CACCTTT 330 0.0 11.512403 14 TCCAACG 165 7.2759576E-12 11.512403 18 TAGGACC 340 0.0 11.456485 4 GGCGAGG 565 0.0 11.430899 19 TGTCGAG 75 2.0776695E-4 11.397279 18 TGCCGGT 110 4.979338E-7 11.224592 10 TAACACC 60 0.0058720433 11.083917 4 GCGTACA 70 0.0014903216 10.857714 2 CCAACGT 70 0.0014939485 10.854551 19 TGTAGAA 615 0.0 10.813577 2 CCGGTTT 115 8.835432E-7 10.736567 12 AGGACGT 1380 0.0 10.670851 5 GGACGTG 1350 0.0 10.623388 6 >>END_MODULE