FastQCFastQC Report
Thu 26 May 2016
SRR1511683_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511683_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536630
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT55870.3635878513370167No Hit
GTATCAACGCAGAGTACTTTTTTTT52180.3395742631602923No Hit
CTGTAGGACGTGGAATATGGCAAGA51130.3327411283132569No Hit
GTCCTAAAGTGTGTATTTCTCATTT50520.32877140235450303No Hit
CTTTAGGACGTGAAATATGGCGAGG41200.2681191959027222No Hit
TATCAACGCAGAGTACTTTTTTTTT31640.20590513005733324No Hit
GGTATCAACGCAGAGTACTTTTTTT31200.20304172116905175No Hit
GTCCTACAGTGTGCATTTCTCATTT24080.1567065591586784No Hit
CTGAAGGACCTGGAATATGGCGAGA23340.1518908260283868No Hit
GTCCTTCAGTGTGCATTTCTCATTT20620.13418975290082844No Hit
ATTTAGAAATGTCCACTGTAGGACG18900.12299642724663712No Hit
TTTCTAAATTTTCCACCTTTTTCAG18250.11876639138894854No Hit
GAATATGGCAAGAAAACTGAAAATC17100.11128248179457643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG601.763897E-514.8284571
GGACCGT400.005442140614.1847916
TAGGACC6000.013.081534
GGTATCA8050.012.5259031
GGCGAGG10550.012.28043919
ACGGGGC550.003175629512.035588
TCCAACG1850.011.75714718
GACCGTG658.337982E-411.6388037
GCCCTAA600.00436746211.5332441
CCAACGA1407.3305273E-1011.483319
CACCTTT5700.011.44737514
ACTATCG855.577028E-511.12532611
GTCCTAT1801.6370905E-1110.9840431
GGCCTAC1353.1672243E-810.9840431
GTCCTAC23850.010.9840421
GTATCAA16850.010.9710051
GAACAGT3800.010.9496626
TTAGGAC15050.010.9331283
GATCTAA1209.1332004E-710.7094411
GGACTAG650.00792408310.6460711
Produced by FastQC (version 0.11.5)