##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511683_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1536630 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.482193501363373 31.0 31.0 34.0 25.0 34.0 2 27.79435583061635 31.0 28.0 34.0 10.0 34.0 3 29.489323389495194 31.0 30.0 34.0 16.0 34.0 4 34.041661297775 37.0 35.0 37.0 30.0 37.0 5 34.501477909451204 37.0 35.0 37.0 32.0 37.0 6 34.76070036378309 37.0 35.0 37.0 32.0 37.0 7 34.83935234897145 37.0 35.0 37.0 32.0 37.0 8 34.86500198486298 37.0 35.0 37.0 32.0 37.0 9 36.58971971131632 39.0 37.0 39.0 33.0 39.0 10 36.48506081490014 39.0 37.0 39.0 33.0 39.0 11 36.50806244834475 39.0 37.0 39.0 33.0 39.0 12 36.44635078060431 39.0 37.0 39.0 33.0 39.0 13 36.45091141003365 39.0 37.0 39.0 33.0 39.0 14 37.74177192948205 40.0 38.0 41.0 33.0 41.0 15 37.71681601947118 40.0 38.0 41.0 33.0 41.0 16 37.67467119605891 40.0 38.0 41.0 33.0 41.0 17 37.667329806134205 40.0 38.0 41.0 33.0 41.0 18 37.63025516877843 40.0 38.0 41.0 33.0 41.0 19 37.66542563922349 40.0 38.0 41.0 33.0 41.0 20 37.624883023239164 40.0 38.0 41.0 33.0 41.0 21 37.553303007230106 40.0 38.0 41.0 33.0 41.0 22 37.48251953951179 40.0 38.0 41.0 32.0 41.0 23 37.399705849814204 40.0 38.0 41.0 32.0 41.0 24 37.34188776738707 40.0 38.0 41.0 32.0 41.0 25 37.266417419938435 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.983410799673333 1101 2 3.578735010856466 1101 3 2.103144898135536 1101 4 1.010197068133742 1101 5 0.44414673384771675 1101 6 0.2019528299327149 1101 7 0.17052189391989714 1101 8 -0.040351320400823454 1101 9 0.1890185130196329 1101 10 0.1249149037263706 1101 11 0.004548185949950323 1101 12 0.1605668562022018 1101 13 0.1193143756111752 1101 14 0.25574863043789264 1101 15 0.11224365517337276 1101 16 0.16735273509366522 1101 17 0.2870643654955174 1101 18 0.17542098118305915 1101 19 0.2172653685432877 1101 20 0.2805435846744899 1101 21 0.37029653339819646 1101 22 0.2426136015374496 1101 23 0.25440435231079306 1101 24 0.29739924125256323 1101 25 0.2489468619670845 1102 1 1.455375237725292 1102 2 4.1344881864182454 1102 3 2.5946700968984935 1102 4 1.4810944385944325 1102 5 0.9824185830923398 1102 6 0.6556729461075363 1102 7 0.6295982630585186 1102 8 0.5374736234001531 1102 9 0.5982946405899199 1102 10 0.5683460428743174 1102 11 0.46714792941934036 1102 12 0.5939121352224817 1102 13 0.5757277012059561 1102 14 0.5600977855721467 1102 15 0.5237987141315656 1102 16 0.6369203937350036 1102 17 0.7105723512073538 1102 18 0.5491315408083253 1102 19 0.5281637364889491 1102 20 0.513605947997938 1102 21 0.658980750169377 1102 22 0.39594192331323086 1102 23 0.6253175296905695 1102 24 0.40104476727103133 1102 25 0.2190134641994419 1103 1 1.221540541331251 1103 2 3.895464624705742 1103 3 2.458328296208144 1103 4 1.3955796367127746 1103 5 0.8552356203199949 1103 6 0.5869329927115672 1103 7 0.40863819489825204 1103 8 0.39596857266024443 1103 9 0.5910573419210081 1103 10 0.2751438229368901 1103 11 0.4105630122413899 1103 12 0.5842618782225131 1103 13 0.48376573318090266 1103 14 0.6363780859722539 1103 15 0.3333322443215252 1103 16 0.384891762957821 1103 17 0.564243354210646 1103 18 0.5362482427912241 1103 19 0.5349659696674962 1103 20 0.44949211216658824 1103 21 0.40941827866636515 1103 22 0.7164598719321518 1103 23 0.6735010327480495 1103 24 0.6428071923512917 1103 25 0.5382774468850968 1104 1 1.2222060469792169 1104 2 4.031622548850386 1104 3 2.4387072081659333 1104 4 1.4571897917222074 1104 5 1.0314728828759883 1104 6 0.8008475522316942 1104 7 0.6239068068792761 1104 8 0.6549236174273076 1104 9 0.7579049457358522 1104 10 0.5119278907393578 1104 11 0.7165128170635171 1104 12 0.730982256404836 1104 13 0.7052773488081883 1104 14 0.7867977231891103 1104 15 0.8062106810552905 1104 16 0.6954728419371747 1104 17 0.7194121595454419 1104 18 0.6928567369166885 1104 19 0.8339919044187596 1104 20 0.7310731969062303 1104 21 0.8509081203927593 1104 22 0.8260344360221126 1104 23 0.8346935666425637 1104 24 0.8667646276392205 1104 25 0.8477494777909769 1105 1 1.3683763515733816 1105 2 3.9254085340293123 1105 3 2.3550057967663776 1105 4 1.3689263549597044 1105 5 0.9750830170370008 1105 6 0.5956849703898044 1105 7 0.6054456639853996 1105 8 0.5847282988293401 1105 9 0.6060210900530691 1105 10 0.5277186693507332 1105 11 0.6227323415770556 1105 12 0.6308417592469198 1105 13 0.6493815187271039 1105 14 0.5099742445529429 1105 15 0.6007303736144465 1105 16 0.4612705076641319 1105 17 0.5987058169711332 1105 18 0.531986572819676 1105 19 0.5887095785939138 1105 20 0.7308142413602638 1105 21 0.6195542971253332 1105 22 0.5674296738112616 1105 23 0.4345539877667264 1105 24 0.27763341431311517 1105 25 0.2821997482250538 1106 1 0.8245756678398806 1106 2 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1107 15 0.7172322219565714 1107 16 0.8076573272021434 1107 17 0.8008975566645091 1107 18 0.7887203552594926 1107 19 0.7950464835121878 1107 20 0.8515700913161695 1107 21 1.0531547174589733 1107 22 1.0654556068956467 1107 23 0.9985497844303239 1107 24 1.0146171451494652 1107 25 0.9679027750025568 1108 1 1.3963701807178204 1108 2 4.127546024623673 1108 3 2.6100285026411676 1108 4 1.5490903817454509 1108 5 1.129112789902635 1108 6 0.7783551510158873 1108 7 0.694244729282282 1108 8 0.6532429451584036 1108 9 0.62137714312545 1108 10 0.6817473078679086 1108 11 0.6842045746176026 1108 12 0.6979051957933606 1108 13 0.6609004491121411 1108 14 0.8113105070260502 1108 15 0.6671797495337444 1108 16 0.7229715009752553 1108 17 0.7179818001676495 1108 18 0.7324240532049018 1108 19 0.7515316862889563 1108 20 0.8450370803319416 1108 21 0.7578453633750613 1108 22 0.9368336493244556 1108 23 1.0183517107512685 1108 24 0.9440219135323034 1108 25 0.9714219254934662 1109 1 1.0628625214081495 1109 2 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-1.7345176289937 2315 9 -1.7568560306753511 2315 10 -1.7957265944968626 2315 11 -1.7052105891954312 2315 12 -1.8496530159792641 2315 13 -1.752667763625766 2315 14 -1.9371122495013466 2315 15 -1.9558889677075655 2315 16 -1.979449867858392 2315 17 -1.9317129814603433 2315 18 -1.9403182424959695 2315 19 -1.9759877987628087 2315 20 -1.9967542821407562 2315 21 -2.1241746798067993 2315 22 -2.136863861489573 2315 23 -2.1075893345401866 2315 24 -1.9580503867969625 2315 25 -2.001554760096546 2316 1 -0.5070723898897107 2316 2 1.9199152378455224 2316 3 0.5359189830677593 2316 4 -0.5074349133454419 2316 5 -0.693410077187373 2316 6 -0.754923999081349 2316 7 -0.9651094771182827 2316 8 -1.000873643278375 2316 9 -1.0856944521667558 2316 10 -1.2809738288872765 2316 11 -1.3254382770370015 2316 12 -1.4900190000380604 2316 13 -1.3172424204707411 2316 14 -1.419166026567268 2316 15 -1.4627174446055875 2316 16 -1.5974827844804693 2316 17 -1.4289745760666293 2316 18 -1.4340203597464836 2316 19 -1.4726477535633862 2316 20 -1.247527465732631 2316 21 -1.4276448554694383 2316 22 -1.337310190092822 2316 23 -1.6482975967903855 2316 24 -1.4490487221702395 2316 25 -1.4744688281259144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31103.0 3 0.0 4 251.0 5 1284.0 6 1718.0 7 1523.0 8 1353.0 9 1334.0 10 1187.0 11 1147.0 12 1106.0 13 1147.0 14 1144.0 15 1190.0 16 1205.0 17 1383.0 18 1557.0 19 1911.0 20 2401.0 21 2948.0 22 3665.0 23 4318.0 24 5221.0 25 6082.0 26 6982.0 27 8000.0 28 9670.0 29 12015.0 30 15546.0 31 21146.0 32 29136.0 33 42692.0 34 64839.0 35 102983.0 36 187799.0 37 252542.0 38 707102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.02230205059123 19.76943050701861 13.445006279976312 25.763261162413855 2 14.845392243965556 21.284604259383475 42.553153856724386 21.316849639926584 3 17.748189219265537 25.119644937297853 30.541184279885204 26.59098156355141 4 11.173411946922812 17.54436656839968 40.14629416320129 31.13592732147622 5 11.737698730338469 38.264448826327744 37.45358349114621 12.54426895218758 6 29.295406181058546 38.976331322439364 19.210610231480576 12.517652265021509 7 26.08604543709286 31.870586933744622 23.94369496886043 18.09967266030209 8 22.529691597847236 39.790515608832315 21.68153686964331 15.998255923677137 9 25.29190501291788 15.6497009690036 21.739260589732076 37.31913342834645 10 13.968814874107624 29.4920703097037 36.36718012794232 20.171934688246356 11 32.5804520281395 22.453420797459376 26.97734653104521 17.988780643355913 12 22.786747623045237 24.57403538913076 34.0217228610661 18.617494126757904 13 28.325035955304788 22.990375041486892 26.69497536817581 21.989613635032505 14 18.88320545609548 23.725294963654232 29.19329962320142 28.198199957048864 15 20.925662000611727 33.92631928310654 24.097538119130828 21.05048059715091 16 19.577386879079544 28.79294299863988 31.13527654672888 20.494393575551694 17 19.646173769873034 29.808411914384074 30.167574497439205 20.37783981830369 18 19.36367245205417 27.883940831559972 33.41481033169989 19.33757638468597 19 22.10811971652252 27.144660718585477 29.144556594625904 21.602662970266103 20 21.9242758503999 28.447837150127224 30.4058882099139 19.22199878955897 21 20.65986299818693 27.605292648419717 28.82784445361318 22.906999899780168 22 21.16036646713757 30.125704631860355 29.205567030973217 19.508361870028857 23 19.77796708227609 29.337656244000627 30.228182783110192 20.656193890613093 24 21.61288013379929 27.310217814307936 31.35829705264117 19.71860499925161 25 20.77448702680541 28.471004731132414 30.527192622817463 20.22731561924471 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 219.0 1 219.0 2 411.5 3 604.0 4 604.0 5 604.0 6 1532.5 7 2461.0 8 2461.0 9 2461.0 10 3455.0 11 4449.0 12 4449.0 13 4449.0 14 7089.0 15 9729.0 16 9729.0 17 9729.0 18 16869.5 19 24010.0 20 24010.0 21 24010.0 22 38142.0 23 52274.0 24 52274.0 25 52274.0 26 75091.0 27 97908.0 28 97908.0 29 97908.0 30 126682.5 31 155457.0 32 155457.0 33 155457.0 34 169207.5 35 182958.0 36 182958.0 37 182958.0 38 188082.5 39 193207.0 40 193207.0 41 193207.0 42 196019.5 43 198832.0 44 198832.0 45 198832.0 46 196091.0 47 193350.0 48 193350.0 49 193350.0 50 176818.0 51 160286.0 52 160286.0 53 160286.0 54 135371.5 55 110457.0 56 110457.0 57 110457.0 58 91500.0 59 72543.0 60 72543.0 61 72543.0 62 57544.0 63 42545.0 64 42545.0 65 42545.0 66 31891.0 67 21237.0 68 21237.0 69 21237.0 70 15163.5 71 9090.0 72 9090.0 73 9090.0 74 6219.5 75 3349.0 76 3349.0 77 3349.0 78 2264.5 79 1180.0 80 1180.0 81 1180.0 82 744.0 83 308.0 84 308.0 85 308.0 86 216.5 87 125.0 88 125.0 89 125.0 90 78.5 91 32.0 92 32.0 93 32.0 94 22.0 95 12.0 96 12.0 97 12.0 98 10.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.337543845948602 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 5.206197978693635E-4 22 7.809296968040452E-4 23 7.158522220703748E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1536630.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.83467367935054 #Duplication Level Percentage of deduplicated Percentage of total 1 89.7046950359504 68.02726275556952 2 7.677417961230995 11.644289715798745 3 1.338325619105386 3.044744598047152 4 0.4409223522540738 1.3374881080447734 5 0.21072100028798668 0.7989979147112901 6 0.1319576350288119 0.6004178515145271 7 0.08177403963820502 0.43409153279838875 8 0.06066676163836781 0.3680515257622851 9 0.048437801908773385 0.33059384113471674 >10 0.2552415517013079 3.5623167084197114 >50 0.024013434191814877 1.2568259413083986 >100 0.020989911500073073 3.2297501972647744 >500 0.002591194052040312 1.4623050279256793 >1k 0.0019002089714962288 2.529312817619266 >5k 3.4549254027204155E-4 1.373551464080694 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5587 0.3635878513370167 No Hit GTATCAACGCAGAGTACTTTTTTTT 5218 0.3395742631602923 No Hit CTGTAGGACGTGGAATATGGCAAGA 5113 0.3327411283132569 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5052 0.32877140235450303 No Hit CTTTAGGACGTGAAATATGGCGAGG 4120 0.2681191959027222 No Hit TATCAACGCAGAGTACTTTTTTTTT 3164 0.20590513005733324 No Hit GGTATCAACGCAGAGTACTTTTTTT 3120 0.20304172116905175 No Hit GTCCTACAGTGTGCATTTCTCATTT 2408 0.1567065591586784 No Hit CTGAAGGACCTGGAATATGGCGAGA 2334 0.1518908260283868 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2062 0.13418975290082844 No Hit ATTTAGAAATGTCCACTGTAGGACG 1890 0.12299642724663712 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1825 0.11876639138894854 No Hit GAATATGGCAAGAAAACTGAAAATC 1710 0.11128248179457643 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 6.507747473367044E-5 0.0 0.0 0.0 5 0.0 6.507747473367044E-5 0.0 0.0 0.0 6 0.0 6.507747473367044E-5 0.0 0.0 0.0 7 0.0 6.507747473367044E-5 0.0 0.0 0.0 8 0.0 6.507747473367044E-5 0.0 0.0 6.507747473367044E-5 9 0.0 6.507747473367044E-5 0.0 0.0 6.507747473367044E-5 10 0.0 6.507747473367044E-5 0.0 6.507747473367044E-5 6.507747473367044E-5 11 0.0 6.507747473367044E-5 0.0 1.3015494946734088E-4 6.507747473367044E-5 12 0.0 6.507747473367044E-5 0.0 1.3015494946734088E-4 6.507747473367044E-5 13 0.0 6.507747473367044E-5 0.0 3.253873736683522E-4 6.507747473367044E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 60 1.763897E-5 14.828457 1 GGACCGT 40 0.0054421406 14.184791 6 TAGGACC 600 0.0 13.08153 4 GGTATCA 805 0.0 12.525903 1 GGCGAGG 1055 0.0 12.280439 19 ACGGGGC 55 0.0031756295 12.03558 8 TCCAACG 185 0.0 11.757147 18 GACCGTG 65 8.337982E-4 11.638803 7 GCCCTAA 60 0.004367462 11.533244 1 CCAACGA 140 7.3305273E-10 11.4833 19 CACCTTT 570 0.0 11.447375 14 ACTATCG 85 5.577028E-5 11.125326 11 GTCCTAT 180 1.6370905E-11 10.984043 1 GGCCTAC 135 3.1672243E-8 10.984043 1 GTCCTAC 2385 0.0 10.984042 1 GTATCAA 1685 0.0 10.971005 1 GAACAGT 380 0.0 10.949662 6 TTAGGAC 1505 0.0 10.933128 3 GATCTAA 120 9.1332004E-7 10.709441 1 GGACTAG 65 0.007924083 10.646071 1 >>END_MODULE