FastQCFastQC Report
Thu 26 May 2016
SRR1511683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536630
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67450.4389475670786071No Hit
CTGTAGGACGTGGAATATGGCAAGA51590.33573469215100576No Hit
TATCAACGCAGAGTACTTTTTTTTT48980.31874947124551783No Hit
GTCCTACAGTGGACATTTCTAAATT46080.2998770035727534No Hit
GGTATCAACGCAGAGTACTTTTTTT44770.29135185438264255No Hit
GTCCTAAAGTGTGTATTTCTCATTT43070.28028868367791854No Hit
CTTTAGGACGTGAAATATGGCGAGG42790.2784665143853758No Hit
CTGAAGGACCTGGAATATGGCGAGA26930.1752536394577745No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22620.1472052478475625No Hit
TTTCTAAATTTTCCACCTTTTTCAG22060.143560909262477No Hit
ATTTAGAAATGTCCACTGTAGGACG19980.13002479451787352No Hit
GAATATGGCAAGAAAACTGAAAATC19160.12468844158971255No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18050.11746484189427513No Hit
GTCCTACAGTGTGCATTTCTCATTT17110.1113475592693101No Hit
CTGTAGGACCTGGAATATGGCGAGA16960.11037139714830506No Hit
GGAATATGGCGAGAAAACTGAAAAT16720.10880953775469697No Hit
GTCCTTCAGTGTGCATTTCTCATTT16030.10431919199807371No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15820.10295256502866662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGCC350.0021742616.28114113
CCAACGA1200.015.82940219
TCCAACG1900.015.49564618
CGTTTCC1101.8735591E-1013.81430114
GGTATCA10800.013.0637461
TAGGACC7150.012.7566434
CCGTTTC1352.7284841E-1112.66310913
CCACCTT6200.012.254621513
ACCGTGC550.00307488112.0875138
TAGAACG951.0383592E-612.0013154
ATCCCGT1353.7289283E-1011.95960210
CCCGTTT1552.5465852E-1111.64189112
GCCTCGA907.4836607E-611.6078516
TCGAACT1157.096605E-811.56234519
TCCCGTT1501.7644197E-1011.39679811
CACCTTT6950.011.34213714
TATAGGT1201.2730015E-711.0845494
CGGTTTC1201.2798955E-711.08022113
CCGGTTT1201.2798955E-711.08022112
ACCTTTT7000.010.98976915
Produced by FastQC (version 0.11.5)