FastQCFastQC Report
Thu 26 May 2016
SRR1511682_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511682_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660854
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34450.5212951726099865No Hit
TATCAACGCAGAGTACTTTTTTTTT21150.3200404325312399No Hit
GGTATCAACGCAGAGTACTTTTTTT20440.30929675843681054No Hit
GTCCTACAGTGGACATTTCTAAATT18830.28493434253254124No Hit
GTCCTAAAGTGTGTATTTCTCATTT18800.28448038447221324No Hit
CTGTAGGACGTGGAATATGGCAAGA17520.2651115072315519No Hit
CTTTAGGACGTGAAATATGGCGAGG13850.20957730451809323No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10030.1517733115029946No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9560.14466130189118928No Hit
CTGAAGGACCTGGAATATGGCGAGA8620.13043728266757862No Hit
GTCCTACAGTGTGCATTTCTCATTT7990.12090416340069061No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7460.11288423766822929No Hit
TTTCTAAATTTTCCACCTTTTTCAG7180.10864729577183463No Hit
GTCCTTCAGTGTGCATTTCTCATTT7080.10713410223740795No Hit
ATTTAGAAATGTCCACTGTAGGACG6820.10319979904789864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG250.00482445619.7465441
TAGGACC1450.015.654514
GACCTAC400.0040834914.8099091
GGTATCA4850.014.6572281
GTCCTAT756.2435174E-714.48081
CGGTCAG500.001548891213.24110516
CTAGGTG500.001548891213.2411054
GTATTAG550.002295718512.5659821
ACGAAAT854.1336807E-612.23967712
GCCTCGA550.00316881212.0373716
CTCGAAC550.00316881212.0373718
TCGAACT550.00316881212.0373719
CTAGCAC550.00316881212.037373
TCCAACG1052.8711838E-711.70982218
TTAGGAC5500.011.6934443
CACCTTT2700.011.55969614
TTTAGGA4900.011.4852352
GGACGTG10100.011.4244346
AGGACGT10350.011.4226255
GGCGAGG4800.011.23129619
Produced by FastQC (version 0.11.5)