##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511682_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 660854 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.331026217591177 31.0 31.0 34.0 25.0 34.0 2 27.718821403819906 31.0 28.0 34.0 10.0 34.0 3 29.36606270068729 31.0 30.0 34.0 16.0 34.0 4 33.86904974472425 37.0 35.0 37.0 28.0 37.0 5 34.307493031743775 37.0 35.0 37.0 32.0 37.0 6 34.56254634155199 37.0 35.0 37.0 32.0 37.0 7 34.62084363565931 37.0 35.0 37.0 32.0 37.0 8 34.65290971984735 37.0 35.0 37.0 32.0 37.0 9 36.35853002327292 39.0 37.0 39.0 33.0 39.0 10 36.2646651151389 39.0 37.0 39.0 32.0 39.0 11 36.28449400321402 39.0 37.0 39.0 32.0 39.0 12 36.229265465594516 39.0 37.0 39.0 32.0 39.0 13 36.21603258813596 39.0 37.0 39.0 32.0 39.0 14 37.517018887681694 40.0 38.0 41.0 32.0 41.0 15 37.47515033577765 40.0 38.0 41.0 32.0 41.0 16 37.42553120659026 40.0 38.0 41.0 32.0 41.0 17 37.43067909099438 40.0 38.0 41.0 32.0 41.0 18 37.390425116591565 40.0 38.0 41.0 32.0 41.0 19 37.42218553568564 40.0 38.0 41.0 32.0 41.0 20 37.38276079133969 40.0 38.0 41.0 32.0 41.0 21 37.31240334476299 40.0 38.0 41.0 32.0 41.0 22 37.23900286598855 40.0 38.0 41.0 32.0 41.0 23 37.1614214334785 40.0 38.0 41.0 32.0 41.0 24 37.10106922255142 40.0 38.0 41.0 32.0 41.0 25 37.016935662037305 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0318780972137773 1101 2 3.6246673779289686 1101 3 2.2594299819659405 1101 4 1.200870308107632 1101 5 0.7999896606433836 1101 6 0.40168431694942797 1101 7 0.30977171572423856 1101 8 0.06619301971987568 1101 9 0.1315390541128565 1101 10 0.3694380773117558 1101 11 -0.005314316840262734 1101 12 0.23749030733331722 1101 13 0.35696737892052255 1101 14 0.4615794556928563 1101 15 0.49543499788990886 1101 16 0.40105305330762775 1101 17 0.5349217839959266 1101 18 0.6281061596325372 1101 19 0.532758730902998 1101 20 0.6956868994919319 1101 21 0.5429756794114056 1101 22 0.645427372281361 1101 23 0.7488077278401732 1101 24 0.61497757320614 1101 25 0.4601200079263137 1102 1 1.5529534367488083 1102 2 4.151467666355458 1102 3 2.7723358788613943 1102 4 1.82853666838718 1102 5 1.2785953879694105 1102 6 1.0957065986882242 1102 7 0.9182802972047241 1102 8 0.9859506305342833 1102 9 1.070541605577965 1102 10 1.092976531535939 1102 11 1.0680304003591488 1102 12 1.1457425537319494 1102 13 1.1074645976650714 1102 14 1.2483221380592227 1102 15 1.3440289187469432 1102 16 1.1463562180752191 1102 17 1.2192968335197065 1102 18 1.2981212940995022 1102 19 1.1707498086989077 1102 20 1.3064933843336917 1102 21 1.2740734433137177 1102 22 1.0688338951573826 1102 23 1.2282097975379571 1102 24 1.1612109926448184 1102 25 1.0326921541680534 1103 1 1.2958342421265883 1103 2 3.9355321965248855 1103 3 2.549593548166442 1103 4 1.3621417656741102 1103 5 0.9749263730902342 1103 6 0.6160667295862936 1103 7 0.539280410047887 1103 8 0.45002418815683853 1103 9 0.5816405367920012 1103 10 0.4345205484301715 1103 11 0.5974320897825152 1103 12 0.692723596080377 1103 13 0.5629701350403451 1103 14 0.5739996000552594 1103 15 0.2963023054927092 1103 16 0.3528128621195208 1103 17 0.45730321721870126 1103 18 0.596250918654178 1103 19 0.5772370816020427 1103 20 0.4825206601385048 1103 21 0.6173423485738212 1103 22 0.6226701597262405 1103 23 0.43555508149972155 1103 24 0.5982304016690492 1103 25 0.4648754275870388 1104 1 1.7428581615712062 1104 2 4.353963176741171 1104 3 2.8721702001747467 1104 4 1.9916308469864248 1104 5 1.3051596013730986 1104 6 1.2813223512747172 1104 7 1.1808154592281497 1104 8 1.0342576883668713 1104 9 0.8811196136195392 1104 10 0.982206224970767 1104 11 1.0774566038141629 1104 12 1.1651889405857219 1104 13 1.2029324710976823 1104 14 1.3595890833733364 1104 15 1.5178783060933867 1104 16 1.1472521543783216 1104 17 1.2602635706428558 1104 18 1.0905759609144994 1104 19 1.253881175607674 1104 20 1.3633962656330567 1104 21 1.3571750118975459 1104 22 1.2560210127455491 1104 23 1.239298747524451 1104 24 1.2378741364872923 1104 25 1.2279923623590179 1105 1 1.629493497069788 1105 2 4.136405001293184 1105 3 2.6404638187279375 1105 4 1.7511644597953477 1105 5 1.3371583108804472 1105 6 0.9864959284377193 1105 7 0.9437916972453593 1105 8 0.8671546279714235 1105 9 0.988608208694032 1105 10 0.7030466976530505 1105 11 0.806097081611199 1105 12 0.9810025902995889 1105 13 0.8542043735710578 1105 14 0.9365947076393155 1105 15 1.0109605966391513 1105 16 0.9020074033981587 1105 17 1.0591454059752081 1105 18 0.9329312673769934 1105 19 0.8927478240448181 1105 20 1.1472131730539843 1105 21 1.2492418263675873 1105 22 1.025888402822524 1105 23 1.0382573042185825 1105 24 1.048997947060279 1105 25 1.1159909774400774 1106 1 1.1585178324103005 1106 2 3.944325041168735 1106 3 2.3248379896622 1106 4 1.3491007393100887 1106 5 0.8076757282954503 1106 6 0.7126449633047116 1106 7 0.6443633003871625 1106 8 0.48828062988480525 1106 9 0.5218632916207042 1106 10 0.29925048100660945 1106 11 0.49672592546144045 1106 12 0.3567170942268518 1106 13 0.3458795486295472 1106 14 0.5961009261348238 1106 15 0.6021879757060944 1106 16 0.5647575300191079 1106 17 0.5037678343640266 1106 18 0.6811585740891104 1106 19 0.5726219266439685 1106 20 0.5433739454819673 1106 21 0.5992341889135133 1106 22 0.5953731560523607 1106 23 0.6593652174249485 1106 24 0.6999441874230214 1106 25 0.6111733518039557 1107 1 1.4987086086637404 1107 2 3.972393628002113 1107 3 2.5212850196885803 1107 4 1.6305391277593202 1107 5 1.3232739817872243 1107 6 1.0905547766631827 1107 7 0.9093992311553123 1107 8 0.9604828675984649 1107 9 1.0496610539070446 1107 10 0.8317204835701446 1107 11 1.0255801102175326 1107 12 0.7702723796469826 1107 13 0.9227565074559507 1107 14 0.9987239962515062 1107 15 0.9980631731392577 1107 16 1.1175249020700093 1107 17 0.8812497035909175 1107 18 1.0055464279182829 1107 19 1.0580346136369414 1107 20 1.1454045093758864 1107 21 1.074012360868494 1107 22 1.1897027929407358 1107 23 1.2264697515653822 1107 24 1.365963769484928 1107 25 1.3133800550216037 1108 1 1.4646896111254293 1108 2 4.114398293611931 1108 3 2.57768476707545 1108 4 1.5123651597999412 1108 5 1.1653585556314567 1108 6 0.8805701138888224 1108 7 0.8042167955007713 1108 8 0.778756076429616 1108 9 0.7689253826134319 1108 10 0.731873855826521 1108 11 0.7937237903783796 1108 12 0.8590865156714145 1108 13 0.8173163033032935 1108 14 0.729890785358208 1108 15 0.9025663888012687 1108 16 0.846397551543518 1108 17 0.9178914955975159 1108 18 1.036923298350949 1108 19 0.9928514046769124 1108 20 0.8616271698045566 1108 21 0.8706587927080562 1108 22 1.0453202129358345 1108 23 1.0629349721507708 1108 24 1.0441655764420972 1108 25 1.206431905154993 1109 1 1.2018873351652601 1109 2 3.4998890475237374 1109 3 2.2449441317032353 1109 4 1.2752069489575248 1109 5 1.061596899238225 1109 6 0.7244929841274796 1109 7 0.7715393812444091 1109 8 0.6448063372167212 1109 9 0.6635831983238774 1109 10 0.5278277044119477 1109 11 0.3989079218782976 1109 12 0.47000006079548484 1109 13 0.5919330252884407 1109 14 0.6540649595323771 1109 15 0.6202050801241725 1109 16 0.6079749183840448 1109 17 0.6046703797448174 1109 18 0.694739008798237 1109 19 0.5457990892035411 1109 20 0.7130575475507257 1109 21 0.6124005344497974 1109 22 0.5505754681910915 1109 23 0.6833316846527495 1109 24 0.7119781258335962 1109 25 0.6575935931587864 1110 1 1.108771099469216 1110 2 3.701612930006892 1110 3 2.225062398059638 1110 4 1.29518672950676 1110 5 0.8595244055350122 1110 6 0.5941995406329994 1110 7 0.48969810393290203 1110 8 0.48498988102407736 1110 9 0.38804524763493475 1110 10 0.28346866971641305 1110 11 0.258265082788526 1110 12 0.35041987585723433 1110 13 0.423806549137808 1110 14 0.29311943875079294 1110 15 0.2800563974388197 1110 16 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3 2.138813692611155 1112 4 1.281426594115615 1112 5 0.694167025117892 1112 6 0.43833974792963915 1112 7 0.4113451102562067 1112 8 0.3341462123282284 1112 9 0.3130033588947967 1112 10 0.2643724561217269 1112 11 0.1730183391644502 1112 12 0.07427204017897537 1112 13 0.07666887621519436 1112 14 -0.07521940958249473 1112 15 0.3724600112372656 1112 16 0.2044165875286552 1112 17 0.18733466758305894 1112 18 0.3611331013743069 1112 19 0.3899858272350656 1112 20 0.5544937652135218 1112 21 0.42266418793379046 1112 22 0.5110274455922195 1112 23 0.4817011960017439 1112 24 0.1738673120760339 1112 25 0.3449263317850182 1113 1 0.7297508600576492 1113 2 3.34646173535279 1113 3 1.686554634009834 1113 4 0.7286033921672086 1113 5 0.3177235305770765 1113 6 -0.05329336836175713 1113 7 -0.1848367741979544 1113 8 0.01662297558684145 1113 9 -0.07832656141270888 1113 10 0.0036984663608095047 1113 11 -0.041960867886920994 1113 12 -0.014826987274346948 1113 13 0.06490553476349703 1113 14 0.044501583839974046 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-1.8517560267178368 2315 13 -1.893070959917047 2315 14 -1.8477642050073513 2315 15 -2.148841067659788 2315 16 -2.4400535243409394 2315 17 -2.204946503197604 2315 18 -2.3503744128365014 2315 19 -2.437624247085111 2315 20 -2.160400983923445 2315 21 -2.363777787823466 2315 22 -2.4704811933709294 2315 23 -2.214806015391133 2315 24 -2.399018802423754 2315 25 -2.2737220619821272 2316 1 -0.8670593157155189 2316 2 1.5916086327854586 2316 3 0.30606959361978525 2316 4 -0.8927961364656483 2316 5 -1.0168481909924694 2316 6 -1.3018945593734017 2316 7 -1.3910014288585515 2316 8 -1.6538698009815391 2316 9 -1.7577654186172467 2316 10 -1.6965561078795162 2316 11 -2.1528715779961303 2316 12 -1.981583909354839 2316 13 -1.9969074296320954 2316 14 -2.273167487638716 2316 15 -2.0947161698309813 2316 16 -2.260697273342714 2316 17 -2.1326022496815114 2316 18 -2.2430054033568965 2316 19 -2.245988662384015 2316 20 -1.9607396755684263 2316 21 -2.1936662685495207 2316 22 -2.168749685183144 2316 23 -2.25326466306727 2316 24 -2.3350925160061777 2316 25 -2.2158861183900243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17215.0 3 0.0 4 148.0 5 759.0 6 857.0 7 748.0 8 653.0 9 681.0 10 625.0 11 588.0 12 548.0 13 579.0 14 615.0 15 554.0 16 591.0 17 626.0 18 712.0 19 897.0 20 1037.0 21 1486.0 22 1708.0 23 1945.0 24 2189.0 25 2609.0 26 2940.0 27 3384.0 28 3967.0 29 4814.0 30 6366.0 31 8636.0 32 11725.0 33 17196.0 34 26285.0 35 42287.0 36 78400.0 37 107363.0 38 309121.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.94195389601939 19.61371195453156 13.68032878669116 26.764005362757885 2 14.700948662300862 20.920641539593937 42.43012974212686 21.94828005597834 3 17.065494042557056 25.13611175842168 30.999282746264683 26.799111452756584 4 11.03768759816843 17.521873212540136 39.98780366011252 31.452635529178913 5 11.662031250472873 37.929557814585365 37.506771541066556 12.901639393875197 6 28.651260338894822 38.776038277743645 19.511268752250878 13.061432631110653 7 25.2461209283745 32.245246302511596 24.249531666601097 18.25910110251281 8 22.334736568137593 38.86319822532662 22.03890723215718 16.763157974378608 9 24.66853495628384 15.436995160807076 22.06856582543194 37.825904057477146 10 13.72859966043937 29.19283230486613 36.126436398962554 20.952131635731945 11 31.444464284093005 22.786424838163953 27.077690382444537 18.69142049529851 12 21.95613554582404 24.787471968089775 34.01295898942883 19.243433496657357 13 27.21645022955146 22.85618306010102 27.362170766916748 22.565195943430773 14 18.72107908857327 23.371122819866414 29.537689111361964 28.37010898019835 15 20.69398081875876 32.725836569045505 25.1212824617843 21.458900150411438 16 19.39051590820363 28.721169880185336 30.867634908769563 21.020679302841476 17 19.171405484418646 29.238833388312695 30.501744712145197 21.088016415123462 18 19.0237177954586 27.498206865661707 33.38846401777095 20.089611321108748 19 21.329976061278284 26.7977495785756 30.154920754054604 21.71735360609151 20 21.14052423076807 27.804477237029662 31.183438399404405 19.871560132797864 21 20.28750851176515 27.113263221608534 29.81039570250435 22.788832564121964 22 20.564851720370555 29.24887190056382 30.195234593233522 19.9910417858321 23 19.36411811526437 28.60666479028397 30.916431362223573 21.112785732228083 24 20.9329457534429 26.943510886687356 31.771816122496226 20.351727237373517 25 20.129256991710726 27.910098145732643 31.252742663281147 20.707902199275484 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 218.0 1 218.0 2 303.0 3 388.0 4 388.0 5 388.0 6 986.5 7 1585.0 8 1585.0 9 1585.0 10 2053.5 11 2522.0 12 2522.0 13 2522.0 14 3599.0 15 4676.0 16 4676.0 17 4676.0 18 8016.0 19 11356.0 20 11356.0 21 11356.0 22 17656.5 23 23957.0 24 23957.0 25 23957.0 26 33741.0 27 43525.0 28 43525.0 29 43525.0 30 55262.5 31 67000.0 32 67000.0 33 67000.0 34 71974.5 35 76949.0 36 76949.0 37 76949.0 38 78580.5 39 80212.0 40 80212.0 41 80212.0 42 80871.0 43 81530.0 44 81530.0 45 81530.0 46 81121.5 47 80713.0 48 80713.0 49 80713.0 50 74659.0 51 68605.0 52 68605.0 53 68605.0 54 58696.5 55 48788.0 56 48788.0 57 48788.0 58 40697.5 59 32607.0 60 32607.0 61 32607.0 62 26045.5 63 19484.0 64 19484.0 65 19484.0 66 14678.5 67 9873.0 68 9873.0 69 9873.0 70 7137.5 71 4402.0 72 4402.0 73 4402.0 74 3007.0 75 1612.0 76 1612.0 77 1612.0 78 1106.0 79 600.0 80 600.0 81 600.0 82 387.0 83 174.0 84 174.0 85 174.0 86 113.5 87 53.0 88 53.0 89 53.0 90 33.5 91 14.0 92 14.0 93 14.0 94 10.5 95 7.0 96 7.0 97 7.0 98 5.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.199414696740884 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 6.052774137706665E-4 22 0.001210554827541333 23 0.001210554827541333 24 1.5131935344266662E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 660854.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.9018673707245 #Duplication Level Percentage of deduplicated Percentage of total 1 90.06917481764847 70.16556910835052 2 7.330219775217141 11.420756174544556 3 1.379797926141812 3.224665051221006 4 0.44844041981571425 1.3973738443262316 5 0.20267437566260932 0.7894356166156494 6 0.1145337536872825 0.5353435973530747 7 0.08297650132951621 0.45248170810211025 8 0.05471465113998689 0.3409898797074176 9 0.04007246750932668 0.280954804421631 >10 0.22857257460437602 3.3731519632474694 >50 0.025198954752891026 1.3837906656201902 >100 0.020277079971798572 3.2472742312119802 >500 0.0017717836865939285 1.010038164027596 >1k 0.0015749188325279366 2.378175191250521 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3445 0.5212951726099865 No Hit TATCAACGCAGAGTACTTTTTTTTT 2115 0.3200404325312399 No Hit GGTATCAACGCAGAGTACTTTTTTT 2044 0.30929675843681054 No Hit GTCCTACAGTGGACATTTCTAAATT 1883 0.28493434253254124 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1880 0.28448038447221324 No Hit CTGTAGGACGTGGAATATGGCAAGA 1752 0.2651115072315519 No Hit CTTTAGGACGTGAAATATGGCGAGG 1385 0.20957730451809323 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1003 0.1517733115029946 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 956 0.14466130189118928 No Hit CTGAAGGACCTGGAATATGGCGAGA 862 0.13043728266757862 No Hit GTCCTACAGTGTGCATTTCTCATTT 799 0.12090416340069061 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 746 0.11288423766822929 No Hit TTTCTAAATTTTCCACCTTTTTCAG 718 0.10864729577183463 No Hit GTCCTTCAGTGTGCATTTCTCATTT 708 0.10713410223740795 No Hit ATTTAGAAATGTCCACTGTAGGACG 682 0.10319979904789864 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.0263870688533324E-4 0.0 6 0.0 0.0 0.0 3.0263870688533324E-4 0.0 7 0.0 0.0 0.0 4.5395806032799983E-4 0.0 8 0.0 0.0 0.0 4.5395806032799983E-4 0.0 9 0.0 0.0 0.0 4.5395806032799983E-4 0.0 10 0.0 0.0 0.0 4.5395806032799983E-4 0.0 11 0.0 0.0 0.0 6.052774137706665E-4 0.0 12 0.0 0.0 0.0 6.052774137706665E-4 0.0 13 0.0 0.0 0.0 6.052774137706665E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 25 0.004824456 19.746544 1 TAGGACC 145 0.0 15.65451 4 GACCTAC 40 0.00408349 14.809909 1 GGTATCA 485 0.0 14.657228 1 GTCCTAT 75 6.2435174E-7 14.4808 1 CGGTCAG 50 0.0015488912 13.241105 16 CTAGGTG 50 0.0015488912 13.241105 4 GTATTAG 55 0.0022957185 12.565982 1 ACGAAAT 85 4.1336807E-6 12.239677 12 GCCTCGA 55 0.003168812 12.03737 16 CTCGAAC 55 0.003168812 12.03737 18 TCGAACT 55 0.003168812 12.03737 19 CTAGCAC 55 0.003168812 12.03737 3 TCCAACG 105 2.8711838E-7 11.709822 18 TTAGGAC 550 0.0 11.693444 3 CACCTTT 270 0.0 11.559696 14 TTTAGGA 490 0.0 11.485235 2 GGACGTG 1010 0.0 11.424434 6 AGGACGT 1035 0.0 11.422625 5 GGCGAGG 480 0.0 11.231296 19 >>END_MODULE