FastQCFastQC Report
Thu 26 May 2016
SRR1511682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660854
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53570.8106177763923651No Hit
TATCAACGCAGAGTACTTTTTTTTT40940.6195014329942772No Hit
GGTATCAACGCAGAGTACTTTTTTT38030.5754675011424611No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18100.27388802973122656No Hit
CTGTAGGACGTGGAATATGGCAAGA16880.2554270686112213No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14500.21941306249186657No Hit
CTTTAGGACGTGAAATATGGCGAGG14220.21517612059547192No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13150.19898494977710657No Hit
GTCCTACAGTGGACATTTCTAAATT11500.1740172564590666No Hit
GTCCTAAAGTGTGTATTTCTCATTT11450.17326065969185325No Hit
CTGAAGGACCTGGAATATGGCGAGA9530.14420734383086128No Hit
TTTCTAAATTTTCCACCTTTTTCAG8480.12831881171938128No Hit
GAATATGGCAAGAAAACTGAAAATC7500.11348951508199996No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7040.1065288248236373No Hit
ATTTAGAAATGTCCACTGTAGGACG6710.1015352861600293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACC456.767098E-414.77430611
GGTATCA7750.014.7631881
GTACTAG400.005149269514.3018371
AGGACCG400.00527778414.2488085
CGAACCG500.001500611513.29687612
GAACCGG500.001500611513.29687613
ATGCCGG801.999717E-613.0594319
ACTGTTC1700.012.8499228
CACTAGA550.003067157512.0898982
GACAGTC550.003070636412.0880697
AACTGCT550.003070636412.0880697
AAGGCGT550.003070636412.0880696
AAATGTC2750.012.0880697
AATGTCC2400.011.87221058
AATGCCG802.8674118E-511.8722118
ATACCTA658.028528E-411.6895616
GGCGAGG5000.011.58727719
TAGGACC2400.011.4782074
TAGAACA2550.011.1755364
GGTATAC600.00571303111.12365151
Produced by FastQC (version 0.11.5)