##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511682_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 660854 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.312837631307367 31.0 28.0 31.0 22.0 34.0 2 28.494163612537715 31.0 28.0 31.0 22.0 34.0 3 28.46160574045099 31.0 28.0 33.0 22.0 34.0 4 31.942480487369373 35.0 32.0 37.0 24.0 37.0 5 31.389753258662278 35.0 32.0 37.0 18.0 37.0 6 31.400578645207563 35.0 32.0 37.0 18.0 37.0 7 31.108029912809787 35.0 32.0 37.0 17.0 37.0 8 30.948050552769597 35.0 32.0 37.0 15.0 37.0 9 31.997574350764314 37.0 32.0 39.0 11.0 39.0 10 31.623974433082044 37.0 30.0 39.0 10.0 39.0 11 31.686776504341353 37.0 31.0 39.0 10.0 39.0 12 31.3728584528504 35.0 30.0 39.0 10.0 39.0 13 31.371619147345708 37.0 30.0 39.0 10.0 39.0 14 31.80083649338583 37.0 30.0 40.0 8.0 41.0 15 31.687290990143058 37.0 30.0 40.0 2.0 41.0 16 31.43242228994604 36.0 30.0 40.0 2.0 41.0 17 31.38794953196924 36.0 30.0 39.0 2.0 41.0 18 31.23592200395246 36.0 30.0 39.0 2.0 41.0 19 31.279343092422835 37.0 30.0 40.0 2.0 41.0 20 31.36308473581154 37.0 30.0 40.0 2.0 41.0 21 31.250777024879927 37.0 30.0 40.0 2.0 41.0 22 31.026400687595142 37.0 30.0 40.0 2.0 41.0 23 30.808726284474332 37.0 30.0 40.0 2.0 41.0 24 30.478989307774487 36.0 29.0 40.0 2.0 41.0 25 29.829074197931767 36.0 27.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.004694220796057635 1101 2 0.15854494582665524 1101 3 0.27680603441242724 1101 4 -0.004424952208328392 1101 5 0.6860113946076574 1101 6 0.2105747903924602 1101 7 0.44943375678021624 1101 8 0.20270117546203537 1101 9 0.02923623232449657 1101 10 0.42353652620017357 1101 11 0.20199404535489762 1101 12 0.2904779458870763 1101 13 0.033067589266611463 1101 14 -0.23979037659350766 1101 15 -0.07582423956775486 1101 16 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2313 16 -0.31122890855081664 2313 17 -0.6356399305532179 2313 18 -0.41708481897691385 2313 19 -0.15745737296705187 2313 20 0.17351341016545518 2313 21 0.2660659885001273 2313 22 0.26019488325761486 2313 23 0.13251658892393792 2313 24 0.4470963700157533 2313 25 0.4448058200079572 2314 1 -0.0061566016055039086 2314 2 -0.05950652983004545 2314 3 0.2005321338641579 2314 4 0.011598887362389121 2314 5 -0.1734963309827684 2314 6 -0.188653180804927 2314 7 -0.491603603899339 2314 8 0.019293431843468767 2314 9 0.1277377753216271 2314 10 -0.9873630782411134 2314 11 -0.18326741577324057 2314 12 -0.24457619157222865 2314 13 -0.2063864760801124 2314 14 -0.38598166581797955 2314 15 0.007457307035000582 2314 16 0.04971693431722457 2314 17 -0.10755885830738521 2314 18 -0.010296108069208998 2314 19 -0.07465253478463652 2314 20 0.302335465303301 2314 21 0.05695625765126522 2314 22 0.37084106392667593 2314 23 0.5228838113277732 2314 24 0.34250881416961576 2314 25 0.1522047521208485 2315 1 -1.0347073045496948 2315 2 -1.2375373649576815 2315 3 -0.775555097998037 2315 4 -1.4156484039132735 2315 5 -1.6833352468021765 2315 6 -1.662014992685318 2315 7 -1.7044097386850012 2315 8 -1.5581962341285056 2315 9 -1.5046563800514932 2315 10 -1.723961081672357 2315 11 -1.9651762460418674 2315 12 -1.7549562765473006 2315 13 -1.7202830091432482 2315 14 -1.517395959592232 2315 15 -1.5883745390426753 2315 16 -1.7139809781515432 2315 17 -1.5921217202862223 2315 18 -1.652304651633326 2315 19 -1.501934368772364 2315 20 -1.515205323882526 2315 21 -1.5759030729109753 2315 22 -1.7608495715440817 2315 23 -1.594957499453006 2315 24 -2.060185517371334 2315 25 -1.7810103683756466 2316 1 -0.7153037024865405 2316 2 -0.8721731657449858 2316 3 -1.069023657454629 2316 4 -0.9425041616712377 2316 5 -1.6746495207055396 2316 6 -1.8097381016961123 2316 7 -1.501382652748454 2316 8 -2.6282410485292402 2316 9 -2.1205232145764334 2316 10 -2.348429086820847 2316 11 -2.321511799739291 2316 12 -2.511781782236003 2316 13 -2.2423613826717457 2316 14 -2.4603139689684923 2316 15 -2.3290734370030073 2316 16 -2.748216549699567 2316 17 -2.3379338756052306 2316 18 -1.9843516456862602 2316 19 -2.080556866165235 2316 20 -1.9495949742926193 2316 21 -1.918547686257586 2316 22 -2.03661315444133 2316 23 -1.938306741742018 2316 24 -2.32958630091872 2316 25 -1.974337062538499 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34518.0 3 3.0 4 505.0 5 3851.0 6 4677.0 7 3781.0 8 3261.0 9 2828.0 10 2699.0 11 2501.0 12 2525.0 13 2351.0 14 2154.0 15 2305.0 16 2239.0 17 2378.0 18 2463.0 19 2956.0 20 3570.0 21 4536.0 22 5562.0 23 6954.0 24 8244.0 25 9455.0 26 11049.0 27 12526.0 28 14285.0 29 16969.0 30 20898.0 31 26512.0 32 32745.0 33 40836.0 34 51472.0 35 64405.0 36 81572.0 37 97559.0 38 73710.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.43958747480944 24.055515648894957 15.913291347814134 19.59160552848146 2 17.251768166644975 25.397894239877495 39.339854188671026 18.010483404806507 3 21.003731535255895 28.809237743888964 31.354429268794622 18.832601452060516 4 15.870525108420317 21.69510966113544 37.895208321353884 24.53915690909036 5 12.530387108297687 40.813784167521895 35.826514208905884 10.829314515274536 6 29.269702536415004 41.256767758082724 17.987028905022896 11.48650080047938 7 26.171741413383288 34.31529505760727 23.66120202041601 15.851761508593427 8 24.336842933537515 40.12610954915912 20.88025494284668 14.656792574456686 9 26.871744742409064 16.09826073535153 21.64002941648231 35.3899651057571 10 15.410816912661495 31.842585503000663 34.418948814715506 18.327648769622336 11 33.563691829057554 23.700091094250773 26.009527066492748 16.726690010198926 12 23.25112657258638 27.20177225226752 32.707980885339275 16.839120289806825 13 29.726535664458414 23.931307066311167 26.595435603022754 19.746721666207666 14 20.63330175802825 24.96481825032458 29.209174795037935 25.19270519660924 15 22.678231500452444 34.18758152330167 24.384054571811625 18.75013240443426 16 20.86164266237323 30.565298840591115 29.55085389511148 19.022204601924177 17 20.56308957803085 31.14500328362997 29.191016472624813 19.100890665714363 18 20.38816440545113 28.739630841305342 32.73885003344157 18.133354719801954 19 23.117360264143063 27.761199901945062 30.355116258659248 18.766323575252628 20 23.013706507034836 28.68001101604893 30.828443196227912 17.477839280688322 21 22.249543772149373 27.792371688754248 30.022062361731937 19.93602217736444 22 22.325354768224145 29.53693251459475 30.49069839934388 17.647014317837222 23 21.331035296752386 29.439936809038002 30.699065149034432 18.529962745175183 24 22.29599881365627 28.38735938649081 31.250472872979508 18.06616892687341 25 21.801561048244807 28.972758830454666 31.043765643016897 18.181914478283634 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 261.0 1 261.0 2 427.0 3 593.0 4 593.0 5 593.0 6 1364.5 7 2136.0 8 2136.0 9 2136.0 10 2801.5 11 3467.0 12 3467.0 13 3467.0 14 4658.5 15 5850.0 16 5850.0 17 5850.0 18 9860.5 19 13871.0 20 13871.0 21 13871.0 22 21128.0 23 28385.0 24 28385.0 25 28385.0 26 39433.0 27 50481.0 28 50481.0 29 50481.0 30 61411.5 31 72342.0 32 72342.0 33 72342.0 34 76708.0 35 81074.0 36 81074.0 37 81074.0 38 80898.5 39 80723.0 40 80723.0 41 80723.0 42 80442.0 43 80161.0 44 80161.0 45 80161.0 46 77672.0 47 75183.0 48 75183.0 49 75183.0 50 68885.0 51 62587.0 52 62587.0 53 62587.0 54 53008.5 55 43430.0 56 43430.0 57 43430.0 58 35879.0 59 28328.0 60 28328.0 61 28328.0 62 22538.5 63 16749.0 64 16749.0 65 16749.0 66 12763.5 67 8778.0 68 8778.0 69 8778.0 70 6371.0 71 3964.0 72 3964.0 73 3964.0 74 2803.5 75 1643.0 76 1643.0 77 1643.0 78 1118.0 79 593.0 80 593.0 81 593.0 82 392.5 83 192.0 84 192.0 85 192.0 86 117.0 87 42.0 88 42.0 89 42.0 90 29.0 91 16.0 92 16.0 93 16.0 94 10.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3590808257194479 2 0.0 3 0.0 4 0.0 5 0.03207970292984532 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.002421109655082666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 660854.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.04580881215605 #Duplication Level Percentage of deduplicated Percentage of total 1 91.40735818387707 71.33961200843137 2 6.274766129558985 9.794383953771062 3 1.1748102347489169 2.750670449153345 4 0.4076398021943744 1.2725831226514903 5 0.19027158541967057 0.7424949889024715 6 0.11439435193477064 0.5356799832174962 7 0.0645796571959334 0.3528120105067878 8 0.05469156097249242 0.3414757689038016 9 0.03997797939545383 0.28081023629345137 >10 0.22053031566944561 3.2112128119600785 >50 0.02636249693841215 1.4595993675900703 >100 0.02074089861545309 3.393908207707013 >500 0.0019384017395080032 1.009821578181639 >1k 0.001744561565557203 2.7045064199480393 >5k 1.9384017395080033E-4 0.8104290927818787 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5357 0.8106177763923651 No Hit TATCAACGCAGAGTACTTTTTTTTT 4094 0.6195014329942772 No Hit GGTATCAACGCAGAGTACTTTTTTT 3803 0.5754675011424611 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1810 0.27388802973122656 No Hit CTGTAGGACGTGGAATATGGCAAGA 1688 0.2554270686112213 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1450 0.21941306249186657 No Hit CTTTAGGACGTGAAATATGGCGAGG 1422 0.21517612059547192 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1315 0.19898494977710657 No Hit GTCCTACAGTGGACATTTCTAAATT 1150 0.1740172564590666 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1145 0.17326065969185325 No Hit CTGAAGGACCTGGAATATGGCGAGA 953 0.14420734383086128 No Hit TTTCTAAATTTTCCACCTTTTTCAG 848 0.12831881171938128 No Hit GAATATGGCAAGAAAACTGAAAATC 750 0.11348951508199996 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 704 0.1065288248236373 No Hit ATTTAGAAATGTCCACTGTAGGACG 671 0.1015352861600293 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGAACC 45 6.767098E-4 14.774306 11 GGTATCA 775 0.0 14.763188 1 GTACTAG 40 0.0051492695 14.301837 1 AGGACCG 40 0.005277784 14.248808 5 CGAACCG 50 0.0015006115 13.296876 12 GAACCGG 50 0.0015006115 13.296876 13 ATGCCGG 80 1.999717E-6 13.05943 19 ACTGTTC 170 0.0 12.849922 8 CACTAGA 55 0.0030671575 12.089898 2 GACAGTC 55 0.0030706364 12.088069 7 AACTGCT 55 0.0030706364 12.088069 7 AAGGCGT 55 0.0030706364 12.088069 6 AAATGTC 275 0.0 12.088069 7 AATGTCC 240 0.0 11.8722105 8 AATGCCG 80 2.8674118E-5 11.87221 18 ATACCTA 65 8.028528E-4 11.689561 6 GGCGAGG 500 0.0 11.587277 19 TAGGACC 240 0.0 11.478207 4 TAGAACA 255 0.0 11.175536 4 GGTATAC 60 0.005713031 11.1236515 1 >>END_MODULE