FastQCFastQC Report
Thu 26 May 2016
SRR1511681_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511681_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751441
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66910.38202828413860357No Hit
GGTATCAACGCAGAGTACTTTTTTT39540.22575696240980997No Hit
TATCAACGCAGAGTACTTTTTTTTT39090.22318764948405342No Hit
GTCCTACAGTGGACATTTCTAAATT35510.2027473377635901No Hit
CTGTAGGACGTGGAATATGGCAAGA33030.1885875687505317No Hit
GTCCTAAAGTGTGTATTTCTCATTT32520.1856756807680076No Hit
CTTTAGGACGTGAAATATGGCGAGG24960.14251122361529736No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18820.10745437613941891No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18060.10311509208703006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG400.0040392414.8362391
GACGTCC400.0054446414.1839687
CGTAGGA602.9112783E-413.1877692
CGGTAGA450.00881615713.1877691
GGTATCA12300.013.1073561
CGGTTTC1450.013.04272813
GTCGAGG751.5506961E-512.6083319
TAGGACC5550.012.2672144
TCCAACG2200.012.03522418
CCAACGA1501.4551915E-1111.97791319
TGCCGGT1451.05501385E-1011.73845610
ATGTCGA752.1649359E-411.34749717
GCCGGTT1501.9099389E-1011.34717411
GACCGTC700.001550478510.8068327
CCGGTTT1502.3028406E-910.71677512
CGCCCTA1159.356918E-710.689671516
GTCCTAC20100.010.6781571
CCGCCCT1252.388024E-710.59069515
CCAACGT1002.5241805E-510.40187319
CCCGTTT1106.389884E-610.31561212
Produced by FastQC (version 0.11.5)