##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511681_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1751441 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.063039520029506 31.0 30.0 34.0 25.0 34.0 2 27.31760647375504 31.0 27.0 34.0 10.0 34.0 3 29.037950464788707 31.0 28.0 34.0 16.0 34.0 4 33.60294180620415 35.0 33.0 37.0 28.0 37.0 5 34.07261220903245 37.0 35.0 37.0 30.0 37.0 6 34.33496475188145 37.0 35.0 37.0 31.0 37.0 7 34.402338988295924 37.0 35.0 37.0 31.0 37.0 8 34.43693164656988 37.0 35.0 37.0 32.0 37.0 9 36.10252529203096 39.0 37.0 39.0 32.0 39.0 10 36.00251335899982 39.0 37.0 39.0 32.0 39.0 11 36.01524230619244 39.0 37.0 39.0 32.0 39.0 12 35.94094691171441 39.0 37.0 39.0 32.0 39.0 13 35.93493471946814 39.0 37.0 39.0 32.0 39.0 14 37.16794627966343 40.0 38.0 41.0 32.0 41.0 15 37.119316608438425 40.0 38.0 41.0 31.0 41.0 16 37.06865718000207 40.0 38.0 41.0 31.0 41.0 17 37.059592644 40.0 38.0 41.0 31.0 41.0 18 37.009077097087484 40.0 38.0 41.0 31.0 41.0 19 37.04905903196283 40.0 38.0 41.0 31.0 41.0 20 36.994518228133295 40.0 38.0 41.0 31.0 41.0 21 36.91922023065579 40.0 38.0 41.0 31.0 41.0 22 36.83376259891141 40.0 37.0 41.0 31.0 41.0 23 36.74649331607516 40.0 37.0 41.0 31.0 41.0 24 36.66207539962808 40.0 37.0 41.0 31.0 41.0 25 36.5716407232673 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9598949558585304 1101 2 3.6354461835034293 1101 3 2.0730000875554815 1101 4 1.0613148315111829 1101 5 0.7860243745698128 1101 6 0.5397442256355944 1101 7 0.3628331387758621 1101 8 0.16096842610016182 1101 9 0.2986596270355264 1101 10 0.5544726919988392 1101 11 0.21856144253684562 1101 12 0.4920679016443117 1101 13 0.43134295219748964 1101 14 0.5362503634255305 1101 15 0.36886079310653486 1101 16 0.4721589304806031 1101 17 0.5113563696765269 1101 18 0.46034211396554525 1101 19 0.5415996780694243 1101 20 0.5750261062418502 1101 21 0.5121412299630634 1101 22 0.42033265327371083 1101 23 0.5894067596564909 1101 24 0.6052903125696005 1101 25 0.5560867253167601 1102 1 1.4083012985650214 1102 2 4.084490291842403 1102 3 2.5156961230887305 1102 4 1.537469827135574 1102 5 1.1995985315083146 1102 6 0.7948945099125737 1102 7 0.6688959714184577 1102 8 0.7649620635795884 1102 9 0.6489126693220726 1102 10 0.7687543766357763 1102 11 0.810392125737259 1102 12 0.7948187561264248 1102 13 0.891882665663914 1102 14 0.6849605219931902 1102 15 0.9040651808240057 1102 16 0.9217479259618884 1102 17 0.8929046472277307 1102 18 0.963271570344304 1102 19 0.9871419233346899 1102 20 1.0017642411867342 1102 21 0.949285195750754 1102 22 0.7363885234694862 1102 23 0.7764372072895327 1102 24 0.7740450252929847 1102 25 0.5949911168869733 1103 1 1.4616909699265257 1103 2 4.115580164411149 1103 3 2.643406384658146 1103 4 1.633258559529942 1103 5 1.0899855201065733 1103 6 0.9717020216496621 1103 7 0.8447282987155731 1103 8 0.7155321507693984 1103 9 0.7916390274709642 1103 10 0.806477381330609 1103 11 0.8993907843556386 1103 12 0.9426255971727002 1103 13 0.9149356502380215 1103 14 1.0809095494915155 1103 15 0.7787224578193133 1103 16 0.8309062089934116 1103 17 0.9219381817683043 1103 18 0.9998738506930565 1103 19 1.0784638549242231 1103 20 1.047849418919796 1103 21 1.0026252359922054 1103 22 1.025874103617042 1103 23 0.9617510905893312 1103 24 1.107417929384205 1103 25 0.9861165360687281 1104 1 1.333695510221645 1104 2 4.006733075064627 1104 3 2.3579528416064193 1104 4 1.5492222371689905 1104 5 1.0212966924756302 1104 6 0.8792843144986975 1104 7 0.6994563674686134 1104 8 0.7763234593473101 1104 9 0.887040357128825 1104 10 0.7872719329764664 1104 11 0.7767762229805797 1104 12 0.7986236513319369 1104 13 0.6814730265960591 1104 14 0.8896788052074243 1104 15 0.8962888596354261 1104 16 0.7049681515911104 1104 17 0.8102034647259799 1104 18 0.7045133836912782 1104 19 0.8499358834632247 1104 20 0.7313655531828118 1104 21 0.7272303924702328 1104 22 0.9426608718998324 1104 23 0.7905436963944297 1104 24 0.7837098054990079 1104 25 0.684232962207858 1105 1 1.330881499802512 1105 2 3.8751195427329392 1105 3 2.430927080422361 1105 4 1.4640680296539585 1105 5 1.1553409718701388 1105 6 0.897474287715049 1105 7 0.8566007419431472 1105 8 0.7568109396242875 1105 9 0.8278252088206202 1105 10 0.6093635143633023 1105 11 0.7826525012182586 1105 12 0.7219588231350116 1105 13 0.7093424790349303 1105 14 0.6159167970354034 1105 15 0.7721294476727465 1105 16 0.712075479407325 1105 17 0.7625508408116275 1105 18 0.7457305103806107 1105 19 0.7479494403647138 1105 20 0.7861306236226469 1105 21 0.7266999662225118 1105 22 0.7991303445576108 1105 23 0.9998937866756634 1105 24 0.9249221973822657 1105 25 0.8993985460542362 1106 1 0.7313441064578896 1106 2 3.435474734785423 1106 3 1.93841323758382 1106 4 0.9701244881892279 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1107 17 0.8660258878524942 1107 18 0.755306439194193 1107 19 0.7744046031039886 1107 20 0.7158691695265418 1107 21 0.8375972001733061 1107 22 0.7675619436036456 1107 23 0.861609488781653 1107 24 1.0146171929209018 1107 25 0.8225503799050315 1108 1 1.4001617545675948 1108 2 4.106905388991798 1108 3 2.4747201615879497 1108 4 1.506919104883309 1108 5 1.08611812206221 1108 6 0.9004499348383064 1108 7 0.7458806955674717 1108 8 0.7442546247339408 1108 9 0.6231900251655347 1108 10 0.5785753223434043 1108 11 0.6644064453779777 1108 12 0.7150065495536495 1108 13 0.7532321761939826 1108 14 0.7862247573776742 1108 15 0.8073880115199188 1108 16 0.8232808015683375 1108 17 0.8454125438002649 1108 18 0.8236550733657708 1108 19 0.7821220153503319 1108 20 0.7815586167892619 1108 21 0.9335959013869086 1108 22 0.9554223983940489 1108 23 0.930060813142795 1108 24 0.8988961072997768 1108 25 0.9949652521828867 1109 1 0.9954197461117502 1109 2 3.073570399093775 1109 3 1.9229899411237312 1109 4 1.1385595518121931 1109 5 0.7229374810452995 1109 6 0.48521911095506454 1109 7 0.36631847039615195 1109 8 0.34174100255707884 1109 9 0.3913366176426436 1109 10 0.31668686313877004 1109 11 0.34927139547549757 1109 12 0.28262874727507636 1109 13 0.25878381530338856 1109 14 0.3478744258499944 1109 15 0.3901579594731146 1109 16 0.29554315969431144 1109 17 0.4278137527243615 1109 18 0.28418067066471053 1109 19 0.39660781460291616 1109 20 0.36017702956714004 1109 21 0.2749641870081092 1109 22 0.39596829167815173 1109 23 0.28742568501365184 1109 24 0.4982706620980153 1109 25 0.4553003445425077 1110 1 0.9910096027820927 1110 2 3.6248383153010515 1110 3 2.093882995147986 1110 4 1.2256598825729412 1110 5 0.7014380323132201 1110 6 0.5816909962913357 1110 7 0.3431652179154483 1110 8 0.31086999924946923 1110 9 0.2893520531442988 1110 10 0.3663009755974258 1110 11 0.3415536446485987 1110 12 0.26278310229161406 1110 13 0.2576812794709724 1110 14 0.06286891317083843 1110 15 0.11397273345326653 1110 16 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-1.5147898044446464 2315 14 -1.6909537061118343 2315 15 -1.7618332738235836 2315 16 -2.094041342134801 2315 17 -1.7872689724087394 2315 18 -1.986954615083107 2315 19 -2.010736658244589 2315 20 -1.9947713644173106 2315 21 -2.033302618509346 2315 22 -1.8608535395797006 2315 23 -1.8603761450909886 2315 24 -1.977475588086179 2315 25 -1.872546810093823 2316 1 -0.16564236340680694 2316 2 2.3483408242306325 2316 3 0.7592675735781569 2316 4 -0.1058569088796446 2316 5 -0.33950592091428433 2316 6 -0.4811955818361753 2316 7 -0.7959464600768342 2316 8 -0.8038111421033065 2316 9 -0.9344705274621177 2316 10 -0.9634136862075167 2316 11 -0.8643178991177507 2316 12 -0.9919651280062638 2316 13 -0.8782314832820504 2316 14 -1.1953058255473863 2316 15 -1.0920531413092291 2316 16 -1.456750186898276 2316 17 -1.2943556246431456 2316 18 -1.3378423024348578 2316 19 -1.2570163816148323 2316 20 -1.1115293548119212 2316 21 -1.3446483401049036 2316 22 -1.1163493635476058 2316 23 -1.2278548851342848 2316 24 -1.3572761260157336 2316 25 -1.1961193805201304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42610.0 3 1.0 4 372.0 5 1866.0 6 2208.0 7 2026.0 8 1928.0 9 1800.0 10 1769.0 11 1773.0 12 1673.0 13 1646.0 14 1618.0 15 1722.0 16 1782.0 17 1954.0 18 2220.0 19 2798.0 20 3575.0 21 4378.0 22 5566.0 23 6582.0 24 7783.0 25 9134.0 26 10366.0 27 12045.0 28 14564.0 29 17718.0 30 23258.0 31 30396.0 32 41504.0 33 58217.0 34 84607.0 35 127597.0 36 215671.0 37 282326.0 38 724388.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.974818449493874 18.77568242378704 12.822698566494676 24.42680056022441 2 15.715574868505843 21.36628505938513 40.59677175648243 22.32136831562659 3 18.088648147439738 25.72898544684063 30.074264562722924 26.10810184299671 4 11.952500826462325 17.738365151894925 39.526766816581315 30.782367205061433 5 12.491485582443257 38.41288401950166 36.44764511051186 12.647985287543229 6 29.84793664188517 38.654684913736745 19.015998826109474 12.481379618268615 7 26.549224324427712 32.41039806650638 23.37418160246334 17.666196006602565 8 23.531195170148468 38.246049966855864 21.55801993901022 16.66473492398545 9 25.758446901722635 15.936020682398095 21.366863057333934 36.93866935854533 10 14.447646252428715 29.528028634707077 35.268730148489155 20.75559496437505 11 32.90764576140447 23.07950995780046 25.801554263032557 18.211290017762515 12 22.68977373488459 25.0622772905282 32.97998619422521 19.267962780361998 13 28.362987962483466 22.701021615915124 26.560928972200603 22.375061449400807 14 20.04697845945139 23.110627192123516 28.77898827308485 28.063406075340247 15 22.009590959672636 31.831332028883647 24.381980323630657 21.777096687813064 16 20.893253041352803 28.81290320370484 29.195274062900207 21.09856969204215 17 20.628042851571934 29.188879328507213 29.080853993939847 21.102223825981007 18 20.318126616882896 27.930087282414878 31.517647468570164 20.234138632132055 19 22.391460740876077 27.390946877997536 28.776092815055037 21.441499566071347 20 22.32007815278962 27.990665971619936 29.699658738147615 19.989597137442825 21 21.841448959596583 27.434059822911028 28.490963986123386 22.233527231369003 22 21.869265387175936 28.89611094045652 28.829079846936157 20.40554382543139 23 21.15470479483575 28.35786382068098 29.302008763145004 21.185422621338272 24 21.974045342144375 27.354820807347558 30.032219220880656 20.638914629627408 25 21.580915371970853 27.94733022693885 29.543273224733234 20.92848117635707 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 320.0 1 320.0 2 517.0 3 714.0 4 714.0 5 714.0 6 1907.0 7 3100.0 8 3100.0 9 3100.0 10 4216.0 11 5332.0 12 5332.0 13 5332.0 14 8277.5 15 11223.0 16 11223.0 17 11223.0 18 19012.0 19 26801.0 20 26801.0 21 26801.0 22 41373.5 23 55946.0 24 55946.0 25 55946.0 26 78492.0 27 101038.0 28 101038.0 29 101038.0 30 128889.5 31 156741.0 32 156741.0 33 156741.0 34 173080.5 35 189420.0 36 189420.0 37 189420.0 38 199090.0 39 208760.0 40 208760.0 41 208760.0 42 215082.5 43 221405.0 44 221405.0 45 221405.0 46 220943.0 47 220481.0 48 220481.0 49 220481.0 50 207488.5 51 194496.0 52 194496.0 53 194496.0 54 169334.0 55 144172.0 56 144172.0 57 144172.0 58 121276.5 59 98381.0 60 98381.0 61 98381.0 62 79197.0 63 60013.0 64 60013.0 65 60013.0 66 45747.5 67 31482.0 68 31482.0 69 31482.0 70 22673.0 71 13864.0 72 13864.0 73 13864.0 74 9562.0 75 5260.0 76 5260.0 77 5260.0 78 3492.5 79 1725.0 80 1725.0 81 1725.0 82 1128.5 83 532.0 84 532.0 85 532.0 86 349.0 87 166.0 88 166.0 89 166.0 90 108.5 91 51.0 92 51.0 93 51.0 94 31.5 95 12.0 96 12.0 97 12.0 98 9.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.398206962152878 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 5.709584279459028E-5 20 0.0 21 2.854792139729514E-4 22 8.564376419188542E-4 23 8.564376419188542E-4 24 1.1419168558918057E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1751441.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.22629642395177 #Duplication Level Percentage of deduplicated Percentage of total 1 85.56906609573942 63.51474864743075 2 10.395197761316954 15.43194060834224 3 2.2411398398522864 4.990545302412107 4 0.7676569180779814 2.27921319812614 5 0.3141845853608319 1.1660379082414742 6 0.17415695164235406 0.7756215310138324 7 0.10765136912125428 0.5593393704386928 8 0.07091547623952969 0.4211034528320831 9 0.04844829424646754 0.32365237049758216 >10 0.27118784875628144 3.5897670902202137 >50 0.019989246490748337 1.0326064376035302 >100 0.01683720265371372 2.4071647339156916 >500 0.0021720759578516173 1.078884181670494 >1k 0.001318760402981339 2.0441046298394907 >5k 7.757414135184346E-5 0.3852705374156629 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6691 0.38202828413860357 No Hit GGTATCAACGCAGAGTACTTTTTTT 3954 0.22575696240980997 No Hit TATCAACGCAGAGTACTTTTTTTTT 3909 0.22318764948405342 No Hit GTCCTACAGTGGACATTTCTAAATT 3551 0.2027473377635901 No Hit CTGTAGGACGTGGAATATGGCAAGA 3303 0.1885875687505317 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3252 0.1856756807680076 No Hit CTTTAGGACGTGAAATATGGCGAGG 2496 0.14251122361529736 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1882 0.10745437613941891 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1806 0.10311509208703006 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.709584279459028E-5 5.709584279459028E-5 5 0.0 0.0 0.0 5.709584279459028E-5 5.709584279459028E-5 6 0.0 0.0 0.0 5.709584279459028E-5 5.709584279459028E-5 7 0.0 0.0 0.0 5.709584279459028E-5 5.709584279459028E-5 8 0.0 0.0 0.0 5.709584279459028E-5 5.709584279459028E-5 9 0.0 0.0 0.0 5.709584279459028E-5 5.709584279459028E-5 10 0.0 0.0 0.0 5.709584279459028E-5 1.7128752838377085E-4 11 0.0 0.0 0.0 5.709584279459028E-5 1.7128752838377085E-4 12 0.0 0.0 0.0 5.709584279459028E-5 1.7128752838377085E-4 13 0.0 0.0 0.0 5.709584279459028E-5 1.7128752838377085E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 40 0.00403924 14.836239 1 GACGTCC 40 0.00544464 14.183968 7 CGTAGGA 60 2.9112783E-4 13.187769 2 CGGTAGA 45 0.008816157 13.187769 1 GGTATCA 1230 0.0 13.107356 1 CGGTTTC 145 0.0 13.042728 13 GTCGAGG 75 1.5506961E-5 12.60833 19 TAGGACC 555 0.0 12.267214 4 TCCAACG 220 0.0 12.035224 18 CCAACGA 150 1.4551915E-11 11.977913 19 TGCCGGT 145 1.05501385E-10 11.738456 10 ATGTCGA 75 2.1649359E-4 11.347497 17 GCCGGTT 150 1.9099389E-10 11.347174 11 GACCGTC 70 0.0015504785 10.806832 7 CCGGTTT 150 2.3028406E-9 10.716775 12 CGCCCTA 115 9.356918E-7 10.6896715 16 GTCCTAC 2010 0.0 10.678157 1 CCGCCCT 125 2.388024E-7 10.590695 15 CCAACGT 100 2.5241805E-5 10.401873 19 CCCGTTT 110 6.389884E-6 10.315612 12 >>END_MODULE