FastQCFastQC Report
Thu 26 May 2016
SRR1511681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511681_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751441
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86800.4955919154570437No Hit
TATCAACGCAGAGTACTTTTTTTTT55560.3172245025667436No Hit
GGTATCAACGCAGAGTACTTTTTTT54120.3090027012043226No Hit
GTCCTACAGTGGACATTTCTAAATT35410.2021763793356442No Hit
CTGTAGGACGTGGAATATGGCAAGA34380.1962955075278014No Hit
GTCCTAAAGTGTGTATTTCTCATTT32180.1837344221129915No Hit
CTTTAGGACGTGAAATATGGCGAGG27080.15461554228775048No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26130.1491914372222644No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22520.12857983797341732No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18290.10442829647130562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.012.8019511
TCCAACG1900.012.49633718
TAGGACC5650.012.443454
GTCGAGG907.48634E-611.60770919
TTAGGAC10550.011.2568043
TATGTCG1104.9878145E-711.22398316
CGAACGG600.005888994311.08008614
GACGTGA9700.011.0637677
TAGACAG3150.010.8579915
AGACCCG700.001490593110.8579915
TGTCGAG1053.4786171E-610.85396318
GTCCTAA9600.010.7281661
GGCGAGG7650.010.67660919
AGGACGT18900.010.3553075
GGACGTG18550.010.2907846
TTTAGGA12450.010.2256982
CCAACGA1606.200935E-910.09079319
ACGTGAA11800.010.0606118
AAATGTC5700.09.997077
TAGGACG20300.09.8751554
Produced by FastQC (version 0.11.5)