##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511681_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1751441 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.237572376117722 31.0 30.0 34.0 26.0 34.0 2 30.44664136559553 31.0 30.0 34.0 27.0 34.0 3 30.523956559198968 31.0 31.0 34.0 27.0 34.0 4 34.11606271635756 37.0 35.0 37.0 30.0 37.0 5 33.693932025115316 37.0 35.0 37.0 28.0 37.0 6 33.64613652415354 37.0 35.0 37.0 29.0 37.0 7 33.42703179838773 37.0 35.0 37.0 28.0 37.0 8 33.312984565280814 36.0 35.0 37.0 27.0 37.0 9 34.701719898072504 38.0 35.0 39.0 27.0 39.0 10 34.45333642412162 38.0 35.0 39.0 27.0 39.0 11 34.454563984741704 38.0 35.0 39.0 27.0 39.0 12 34.25294885754074 38.0 34.0 39.0 27.0 39.0 13 34.23757694378514 38.0 34.0 39.0 27.0 39.0 14 35.094895003599895 39.0 34.0 40.0 26.0 41.0 15 34.9946021590222 38.0 34.0 40.0 25.0 41.0 16 34.81536746027985 38.0 34.0 40.0 25.0 41.0 17 34.77795483833027 38.0 34.0 40.0 25.0 41.0 18 34.68871403604232 38.0 34.0 40.0 25.0 41.0 19 34.713164188802246 39.0 34.0 40.0 25.0 41.0 20 34.80988625937157 39.0 34.0 40.0 25.0 41.0 21 34.74080028959011 39.0 34.0 40.0 24.0 41.0 22 34.61008906380518 39.0 34.0 40.0 24.0 41.0 23 34.474584071059205 39.0 34.0 40.0 22.0 41.0 24 34.27275540540618 39.0 34.0 40.0 19.0 41.0 25 33.70492925539599 38.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26777680655677827 1101 2 0.359282620301439 1101 3 0.44313578349449756 1101 4 0.47714284625551784 1101 5 0.6712660768963516 1101 6 0.5358012596044333 1101 7 0.5656275601270195 1101 8 0.27446345362700697 1101 9 0.44068909614271945 1101 10 0.5505811350259151 1101 11 0.5265975537133087 1101 12 0.6880179115303235 1101 13 0.6283605460059576 1101 14 0.5033075624773531 1101 15 0.32317129929086974 1101 16 0.37301539283662066 1101 17 0.266631668286351 1101 18 0.4448303388818786 1101 19 0.2540476671716547 1101 20 0.10224563693740407 1101 21 0.06171546022636676 1101 22 0.40617671815591194 1101 23 0.37083933875517516 1101 24 -0.005015908389431445 1101 25 0.19053175290292046 1102 1 0.3305839935631454 1102 2 0.39428267534054484 1102 3 0.4166984372599991 1102 4 0.5799613988371277 1102 5 0.5508102430263833 1102 6 0.5964770572586673 1102 7 0.5568364390354787 1102 8 0.2370292941566987 1102 9 0.5001333938162205 1102 10 0.5500314319618909 1102 11 0.5976145112616464 1102 12 0.7801127824360989 1102 13 0.6446720728457791 1102 14 0.7804542251812023 1102 15 0.6029130558088767 1102 16 0.34098882546049936 1102 17 0.7262116373543748 1102 18 0.7349332069696572 1102 19 0.7874887395535808 1102 20 0.7213062294884125 1102 21 0.4541202012948986 1102 22 0.6474289403597879 1102 23 0.8859496371221667 1102 24 0.6232706141848539 1102 25 0.7855065449926997 1103 1 0.19091061998836523 1103 2 0.3489500126980225 1103 3 0.45746906100245255 1103 4 0.6443074752958751 1103 5 0.7384936769633441 1103 6 0.7045930867690657 1103 7 0.47166273011529114 1103 8 0.5180816080399993 1103 9 0.5130046211804 1103 10 0.746356717005483 1103 11 0.4538640082435421 1103 12 0.6403307569498509 1103 13 0.38408253562244 1103 14 0.6817795102247999 1103 15 0.7083376030925805 1103 16 0.6727936544343436 1103 17 0.7890694509023319 1103 18 0.6451867280964194 1103 19 0.7262539977695397 1103 20 0.7374779263361617 1103 21 0.8157332126966423 1103 22 0.8147119718822537 1103 23 0.7871508443656268 1103 24 0.6822954297448831 1103 25 0.7508660352676841 1104 1 0.3230228301391662 1104 2 0.4115399267567206 1104 3 0.49953684112244545 1104 4 0.6078586346926684 1104 5 0.3507148541970082 1104 6 0.48758124957107185 1104 7 0.6765480876635337 1104 8 0.681420670793365 1104 9 0.796417625882853 1104 10 0.7328895530684036 1104 11 0.6881406419390146 1104 12 0.8503161631441145 1104 13 0.6758886001594888 1104 14 0.8221375449256243 1104 15 1.0183820331233875 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0.801824271332535 1107 14 0.8351007923404552 1107 15 0.8935638144910243 1107 16 1.1233297662428612 1107 17 0.8582765812061197 1107 18 1.0797643496547096 1107 19 0.8527636638503822 1107 20 0.840418088692843 1107 21 0.895441974936162 1107 22 0.8455165580400958 1107 23 0.7534556397459937 1107 24 1.009453853043901 1107 25 0.8595894430842392 1108 1 0.1750851966637761 1108 2 0.1202040074511821 1108 3 0.06986941079178521 1108 4 0.35208551806557153 1108 5 0.3625191161874426 1108 6 0.3500383526884505 1108 7 0.4699643439160752 1108 8 0.37677021603745686 1108 9 0.5106193759303821 1108 10 0.66250076661197 1108 11 0.6581575132916342 1108 12 0.6625312498348208 1108 13 0.42988236072777397 1108 14 0.5844232815897712 1108 15 0.8941574615869001 1108 16 0.8441249208307156 1108 17 0.7626504986803724 1108 18 0.6294404843568486 1108 19 0.6111688558663673 1108 20 0.8031327478641046 1108 21 0.7988193085707209 1108 22 0.7866623653952516 1108 23 0.7478389753776327 1108 24 0.9018785444598834 1108 25 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2313 20 -0.32800421652098066 2313 21 -0.1733739301605013 2313 22 -0.1433207662129803 2313 23 -0.22605153658675903 2313 24 -0.1061748083503602 2313 25 -0.031116107589461706 2314 1 -0.4608268647491194 2314 2 -0.6477300849820757 2314 3 -0.7176084725750798 2314 4 -0.7359092524208535 2314 5 -0.6170301325604655 2314 6 -0.8522860341100582 2314 7 -0.770191970739404 2314 8 -0.7589831050247113 2314 9 -0.8564935473177684 2314 10 -2.0312855296367616 2314 11 -1.4039955766160475 2314 12 -1.1315129548938572 2314 13 -0.9928851078452041 2314 14 -1.0198798304345402 2314 15 -0.9040915788365993 2314 16 -0.6074432198025335 2314 17 -0.7442199263870464 2314 18 -0.84834745993777 2314 19 -0.7409511304355902 2314 20 -0.7727424643842298 2314 21 -0.867551280587854 2314 22 -0.8250114713411847 2314 23 -0.7627502545354758 2314 24 -0.7415647656153155 2314 25 -0.8706193127176647 2315 1 -0.8836718266194374 2315 2 -0.8971086998519731 2315 3 -0.9062547334281561 2315 4 -1.0524203188328656 2315 5 -1.3733692767759962 2315 6 -1.3403798209360147 2315 7 -1.2087324098741377 2315 8 -1.222810701974339 2315 9 -1.3133538423317859 2315 10 -1.431943486752722 2315 11 -1.280167234681521 2315 12 -1.3085078212706591 2315 13 -1.2843799200412889 2315 14 -1.5318825530157127 2315 15 -1.6031760939868818 2315 16 -1.7373381092061422 2315 17 -1.8192737684773377 2315 18 -1.554297688174053 2315 19 -1.541760793566965 2315 20 -1.756026865754997 2315 21 -1.7232781826697448 2315 22 -1.8250800438146726 2315 23 -1.85284432581021 2315 24 -1.6004316097238416 2315 25 -1.5536646416623583 2316 1 -1.0533147145899129 2316 2 -0.9939886738877242 2316 3 -1.15642823124778 2316 4 -1.0959415131884072 2316 5 -1.0756357970484842 2316 6 -1.204640651170557 2316 7 -1.3081716925426576 2316 8 -2.1360989086111033 2316 9 -1.7145671186266327 2316 10 -1.6428365603054402 2316 11 -1.4782162905426404 2316 12 -1.653173755966428 2316 13 -1.5577313454855641 2316 14 -1.42793664290312 2316 15 -1.7602693063378538 2316 16 -1.7815256732967484 2316 17 -1.6566130887802117 2316 18 -1.507126997393776 2316 19 -1.5117111438426605 2316 20 -1.7872702840591046 2316 21 -1.67398667525854 2316 22 -1.5143549437728936 2316 23 -1.5930041509658395 2316 24 -1.5475078693525361 2316 25 -1.5373592993105945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41246.0 3 1.0 4 739.0 5 6115.0 6 7259.0 7 6046.0 8 4932.0 9 4336.0 10 4066.0 11 3957.0 12 3630.0 13 3513.0 14 3441.0 15 3478.0 16 3509.0 17 3695.0 18 3891.0 19 4545.0 20 5472.0 21 6872.0 22 8872.0 23 11248.0 24 13293.0 25 15779.0 26 18179.0 27 20660.0 28 24191.0 29 29010.0 30 36536.0 31 46514.0 32 59496.0 33 77322.0 34 99762.0 35 135166.0 36 198987.0 37 332032.0 38 503651.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.829086889998344 21.27022399092869 14.52120240701536 23.379486712057606 2 15.065480367308975 22.33018411696426 41.01793894284763 21.58639657287913 3 18.839001713446244 26.609688821947188 30.88371232602183 23.667597138584743 4 12.966465898651453 19.014114663297253 39.04681916204999 28.9726002760013 5 12.603989970814844 38.75509026837779 36.367904322944334 12.27301543786303 6 30.057364193255726 39.350112278974855 18.38389075053056 12.208632777238856 7 26.643032794139227 32.749204797649476 23.321824714620703 17.28593769359059 8 24.101354256295245 38.513144319448955 21.093773641247406 16.29172778300839 9 26.270596611590115 15.588592478993013 21.083325102015998 37.05748580740087 10 14.52421177761626 30.15385616757858 35.14443249872534 20.177499556079823 11 33.53655646978687 23.110969767180283 25.47439508381955 17.87807867921329 12 22.763370276246818 25.561923010823655 32.887719312269155 18.786987400660372 13 28.787152978604475 22.80270931193229 26.540545756322935 21.8695919531403 14 20.321780750821752 23.246058531232283 28.804738498185205 27.627422219760756 15 22.344914844405263 32.18789556713586 24.196875601290593 21.27031398716828 16 21.166685032496098 29.185910344681893 28.92275560524163 20.724649017580383 17 20.811777273684925 29.50056553432288 28.916988925119373 20.77066826687282 18 20.555131460323242 28.155044903025566 31.39249338116442 19.897330255486768 19 22.657400392020058 27.588768334188817 28.88387333629851 20.869957937492615 20 22.679781962395538 28.030005007305412 29.641991936925084 19.648221093373973 21 22.11984303210899 27.51625661383969 28.625400455967398 21.738499898083923 22 22.176596299846814 28.876222493364033 28.91607539163466 20.031105815154493 23 21.480826359551937 28.493566154954692 29.33938397011375 20.68622351537962 24 22.173627316021495 27.59676175218006 29.996500024836692 20.233110906961755 25 21.920356468296294 28.087865506759147 29.579982688174745 20.411795336769814 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 333.0 1 333.0 2 613.5 3 894.0 4 894.0 5 894.0 6 2153.0 7 3412.0 8 3412.0 9 3412.0 10 4660.0 11 5908.0 12 5908.0 13 5908.0 14 8804.5 15 11701.0 16 11701.0 17 11701.0 18 19891.0 19 28081.0 20 28081.0 21 28081.0 22 43596.5 23 59112.0 24 59112.0 25 59112.0 26 83141.0 27 107170.0 28 107170.0 29 107170.0 30 134418.5 31 161667.0 32 161667.0 33 161667.0 34 177619.0 35 193571.0 36 193571.0 37 193571.0 38 201306.0 39 209041.0 40 209041.0 41 209041.0 42 215067.0 43 221093.0 44 221093.0 45 221093.0 46 218902.0 47 216711.0 48 216711.0 49 216711.0 50 203442.5 51 190174.0 52 190174.0 53 190174.0 54 165446.0 55 140718.0 56 140718.0 57 140718.0 58 117631.5 59 94545.0 60 94545.0 61 94545.0 62 75887.5 63 57230.0 64 57230.0 65 57230.0 66 43555.0 67 29880.0 68 29880.0 69 29880.0 70 21426.5 71 12973.0 72 12973.0 73 12973.0 74 8960.5 75 4948.0 76 4948.0 77 4948.0 78 3284.5 79 1621.0 80 1621.0 81 1621.0 82 1064.0 83 507.0 84 507.0 85 507.0 86 313.0 87 119.0 88 119.0 89 119.0 90 72.0 91 25.0 92 25.0 93 25.0 94 16.0 95 7.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40195473327391557 2 0.0 3 0.0 4 0.0 5 0.031459809379819244 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0021125461833998403 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1751441.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.55909354316475 #Duplication Level Percentage of deduplicated Percentage of total 1 85.77108213657617 63.09243054182873 2 10.191397759573007 14.99339962264061 3 2.251228624940765 4.967950110272038 4 0.7363867004161717 2.1667175271942245 5 0.3282073993685739 1.207131939585588 6 0.16443618271069455 0.7257465927538147 7 0.1098163280486516 0.5654592683248342 8 0.07289554772314448 0.42897043310776123 9 0.05288120441696755 0.35009041161446397 >10 0.2789495011774709 3.659270861471274 >50 0.020159558523081048 1.0173722819827187 >100 0.018619756976317105 2.626276488638645 >500 0.002394476196464865 1.2405052707241313 >1k 0.0013130998496742807 1.8423204966440687 >5k 2.317235028836966E-4 1.1163581532170441 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8680 0.4955919154570437 No Hit TATCAACGCAGAGTACTTTTTTTTT 5556 0.3172245025667436 No Hit GGTATCAACGCAGAGTACTTTTTTT 5412 0.3090027012043226 No Hit GTCCTACAGTGGACATTTCTAAATT 3541 0.2021763793356442 No Hit CTGTAGGACGTGGAATATGGCAAGA 3438 0.1962955075278014 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3218 0.1837344221129915 No Hit CTTTAGGACGTGAAATATGGCGAGG 2708 0.15461554228775048 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2613 0.1491914372222644 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2252 0.12857983797341732 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1829 0.10442829647130562 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.709584279459028E-5 0.0 4 0.0 0.0 0.0 5.709584279459028E-5 0.0 5 0.0 0.0 0.0 5.709584279459028E-5 0.0 6 0.0 0.0 0.0 5.709584279459028E-5 0.0 7 0.0 0.0 0.0 5.709584279459028E-5 0.0 8 0.0 0.0 0.0 5.709584279459028E-5 0.0 9 0.0 0.0 0.0 1.7128752838377085E-4 0.0 10 0.0 0.0 0.0 1.7128752838377085E-4 0.0 11 0.0 0.0 0.0 1.7128752838377085E-4 0.0 12 0.0 0.0 0.0 1.7128752838377085E-4 0.0 13 0.0 0.0 0.0 1.7128752838377085E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1460 0.0 12.801951 1 TCCAACG 190 0.0 12.496337 18 TAGGACC 565 0.0 12.44345 4 GTCGAGG 90 7.48634E-6 11.607709 19 TTAGGAC 1055 0.0 11.256804 3 TATGTCG 110 4.9878145E-7 11.223983 16 CGAACGG 60 0.0058889943 11.080086 14 GACGTGA 970 0.0 11.063767 7 TAGACAG 315 0.0 10.857991 5 AGACCCG 70 0.0014905931 10.857991 5 TGTCGAG 105 3.4786171E-6 10.853963 18 GTCCTAA 960 0.0 10.728166 1 GGCGAGG 765 0.0 10.676609 19 AGGACGT 1890 0.0 10.355307 5 GGACGTG 1855 0.0 10.290784 6 TTTAGGA 1245 0.0 10.225698 2 CCAACGA 160 6.200935E-9 10.090793 19 ACGTGAA 1180 0.0 10.060611 8 AAATGTC 570 0.0 9.99707 7 TAGGACG 2030 0.0 9.875155 4 >>END_MODULE