FastQCFastQC Report
Thu 26 May 2016
SRR1511680_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511680_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920596
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43920.4770822380284077No Hit
TATCAACGCAGAGTACTTTTTTTTT30300.3291346041042976No Hit
GGTATCAACGCAGAGTACTTTTTTT28450.3090389269560154No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11680.12687432923888436No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11590.12589670170194092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6550.014.7929611
TGGACCG400.005440284514.1846595
GTATTAG550.002272885312.5836221
GTCCAAT550.002272885312.5836221
GTGGTAT1801.8189894E-1211.5349871
CCCTATA600.004360169611.53498652
TTGGACT700.001548639710.8073594
CTAGGAC700.001548639710.8073593
GGTCTAC1852.7284841E-1110.6887911
AAGGCGT803.930934E-410.63849456
TAAGACT1605.875336E-1010.63849454
GTCCTAC1904.5474735E-1110.4075071
GTAATAC1002.5200527E-510.4020833
AGACTGT1553.936293E-910.3715796
GTCTTAT1353.7176142E-710.2533221
GTATCAA13100.010.1135551
TTGGACC750.002748613710.0868684
CCTAGAC750.002748613710.0868683
AAAGGCG856.871728E-410.01275
GGATAGA907.7154796E-49.8871321
Produced by FastQC (version 0.11.5)