##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511680_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 920596 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.408954633737274 31.0 30.0 34.0 25.0 34.0 2 27.691993013221868 31.0 28.0 34.0 10.0 34.0 3 29.391633246288276 31.0 30.0 34.0 16.0 34.0 4 33.987075763961606 37.0 35.0 37.0 30.0 37.0 5 34.44761763031775 37.0 35.0 37.0 32.0 37.0 6 34.7100660876215 37.0 35.0 37.0 32.0 37.0 7 34.786694706472765 37.0 35.0 37.0 32.0 37.0 8 34.82184584768997 37.0 35.0 37.0 32.0 37.0 9 36.537944983467234 39.0 37.0 39.0 33.0 39.0 10 36.45528983397712 39.0 37.0 39.0 33.0 39.0 11 36.47542678873252 39.0 37.0 39.0 33.0 39.0 12 36.40741106848172 39.0 37.0 39.0 33.0 39.0 13 36.398933951483606 39.0 37.0 39.0 33.0 39.0 14 37.703426910392835 40.0 38.0 41.0 33.0 41.0 15 37.650176624708344 40.0 38.0 41.0 33.0 41.0 16 37.598912009176665 40.0 38.0 41.0 33.0 41.0 17 37.588072292297596 40.0 38.0 41.0 33.0 41.0 18 37.545819230150904 40.0 38.0 41.0 33.0 41.0 19 37.59211315278363 40.0 38.0 41.0 33.0 41.0 20 37.532689692329754 40.0 38.0 41.0 32.0 41.0 21 37.47383651460576 40.0 38.0 41.0 32.0 41.0 22 37.39372645547015 40.0 38.0 41.0 32.0 41.0 23 37.313437164619444 40.0 38.0 41.0 32.0 41.0 24 37.23420045275017 40.0 38.0 41.0 32.0 41.0 25 37.14894155525334 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5989847367454537 1101 2 3.2806976364092897 1101 3 1.8846083751587344 1101 4 0.7877145839476256 1101 5 0.4424890834893844 1101 6 0.11724767898224542 1101 7 0.09151880270289325 1101 8 -0.05969287623332775 1101 9 -0.15144388978531964 1101 10 -0.029542444928324585 1101 11 -0.16208462558991954 1101 12 -0.028728532896252545 1101 13 -0.08400877898741044 1101 14 0.05451647553530137 1101 15 -0.02979918154068173 1101 16 0.09754541600133138 1101 17 -0.09224980468384558 1101 18 0.043993891455670564 1101 19 -0.07333034122104465 1101 20 0.01653304750694673 1101 21 0.019423202016476182 1101 22 0.12829823173120047 1101 23 0.008503061069816908 1101 24 0.1064086523076142 1101 25 0.05453353971150676 1102 1 1.4676665321502291 1102 2 4.290346034761246 1102 3 2.8336938768104893 1102 4 1.529610068525038 1102 5 1.177819295805186 1102 6 0.9807526528139547 1102 7 0.8882192314174944 1102 8 0.7877109413680969 1102 9 0.9417980555767684 1102 10 0.8074764110903843 1102 11 0.7730131343299007 1102 12 0.8858498513508053 1102 13 0.8715410799636274 1102 14 0.8405755954640313 1102 15 1.0771787981185739 1102 16 1.0246871330868146 1102 17 1.1096102784608064 1102 18 0.8883853394345849 1102 19 1.0380832570121328 1102 20 0.9642973751117125 1102 21 1.0102956176654843 1102 22 0.8941581200055779 1102 23 0.9145291922453538 1102 24 0.7852833228840552 1102 25 0.7109598509100508 1103 1 1.4041630241740677 1103 2 4.130852158204995 1103 3 2.6940540846939065 1103 4 1.4928964859211646 1103 5 1.0055561152250903 1103 6 0.7494270688470621 1103 7 0.6989634098018627 1103 8 0.6669232771679532 1103 9 0.6244114019084535 1103 10 0.5546854882748846 1103 11 0.6097720757763767 1103 12 0.6449887554721414 1103 13 0.7276802074941884 1103 14 0.8967302939405641 1103 15 0.7762240057650374 1103 16 0.7989930412453106 1103 17 0.896983369609508 1103 18 1.0612171980413692 1103 19 0.9057934027245906 1103 20 0.8669518829005725 1103 21 0.9665220778930035 1103 22 1.0345663044558506 1103 23 0.8021667638096446 1103 24 0.979090825372019 1103 25 0.7183921567866207 1104 1 1.2271521871111801 1104 2 3.8950523908502497 1104 3 2.3938168391085775 1104 4 1.3482226821712402 1104 5 0.8265342768018158 1104 6 0.7334310645621827 1104 7 0.6703132123162732 1104 8 0.6194150130038665 1104 9 0.5957152011237739 1104 10 0.5379862907038557 1104 11 0.6262839742010939 1104 12 0.6553427837181616 1104 13 0.3846106045026616 1104 14 0.6346823585029071 1104 15 0.6672684778114615 1104 16 0.6546248308393672 1104 17 0.5360809163192854 1104 18 0.5285811432842991 1104 19 0.6932253014121841 1104 20 0.774859850014785 1104 21 0.7781418540540912 1104 22 0.8890414396726882 1104 23 0.9178552031805793 1104 24 0.770528297030701 1104 25 0.8881607601921786 1105 1 1.2990447989274791 1105 2 3.950002328325624 1105 3 2.50614596701854 1105 4 1.5188656580007631 1105 5 1.070403865852299 1105 6 0.8195159096210247 1105 7 0.7887170785649644 1105 8 0.7081667902449027 1105 9 0.6649954827415314 1105 10 0.4687616816968898 1105 11 0.5058245038617457 1105 12 0.7303691211455785 1105 13 0.7826708422675352 1105 14 0.7583569221152899 1105 15 0.7338914628912008 1105 16 0.7800284572675835 1105 17 0.7994969448921836 1105 18 0.8463539819022543 1105 19 0.6676073354324359 1105 20 0.8277081464328973 1105 21 0.7407170120786546 1105 22 0.6490196845293923 1105 23 0.6996067919912434 1105 24 0.800444830939604 1105 25 0.5538799218483064 1106 1 0.7990334019289911 1106 2 3.516584733183482 1106 3 2.04332639518098 1106 4 0.9463283214337253 1106 5 0.33733752620351254 1106 6 0.39568205050616 1106 7 0.1450157437485018 1106 8 0.16510551086344094 1106 9 0.13550688885761275 1106 10 0.1015754638592199 1106 11 0.07098684270818723 1106 12 -0.019447387178793463 1106 13 0.04783910755318743 1106 14 0.19887826641405582 1106 15 0.36164269832583784 1106 16 0.1997423014295876 1106 17 0.27336476706253876 1106 18 0.11212514840784138 1106 19 0.16961320716605144 1106 20 0.016845895115402243 1106 21 -0.06299476238842061 1106 22 -0.06528491621095611 1106 23 -0.035156560732183095 1106 24 0.12358050992718717 1106 25 -0.1298727450493402 1107 1 1.113728177135446 1107 2 3.620359081674181 1107 3 2.2472015718036538 1107 4 1.1936509413926046 1107 5 0.8324475930599604 1107 6 0.7302082854773388 1107 7 0.5900424423903274 1107 8 0.44664494368207386 1107 9 0.5597204409910788 1107 10 0.5900808255570169 1107 11 0.5686823648753432 1107 12 0.47945529748584903 1107 13 0.5906251553447959 1107 14 0.40115198704150146 1107 15 0.7259247950884671 1107 16 0.6180217942363342 1107 17 0.5688235697515935 1107 18 0.49092557114597213 1107 19 0.6326051201023475 1107 20 0.7265996293407682 1107 21 0.7093360093861776 1107 22 0.7763756028689173 1107 23 0.6475273638872281 1107 24 0.6593559962032316 1107 25 0.8080485766317196 1108 1 1.5546832267861497 1108 2 4.283397179494887 1108 3 2.7527412729955323 1108 4 1.6069634416616267 1108 5 1.3303018735229415 1108 6 1.075273130542179 1108 7 0.9053061054800793 1108 8 0.8482259322195844 1108 9 0.8947539934159394 1108 10 0.8497946722273824 1108 11 1.0106080459314626 1108 12 0.9872612400744032 1108 13 0.9872346171716515 1108 14 0.9732111460571815 1108 15 1.0411497190090486 1108 16 1.0374593875869706 1108 17 1.1976721986002232 1108 18 1.1451397470992788 1108 19 1.0261757093251092 1108 20 1.0951834544463352 1108 21 1.2003528264899543 1108 22 1.2073725338658505 1108 23 1.0546050100953082 1108 24 1.0967126074728881 1108 25 1.119918258066619 1109 1 1.1141563958952823 1109 2 3.26671675995771 1109 3 2.043329831886602 1109 4 1.2145929798423651 1109 5 0.6559144093177167 1109 6 0.5893724881018869 1109 7 0.34156208371429386 1109 8 0.5059511008255129 1109 9 0.6258331353221038 1109 10 0.3755988953181344 1109 11 0.4737034439437977 1109 12 0.5074843564108917 1109 13 0.48071852844537943 1109 14 0.6546232353634167 1109 15 0.40217749554302173 1109 16 0.5821284977869041 1109 17 0.6746963759333653 1109 18 0.4321557579992259 1109 19 0.7213545286251843 1109 20 0.5658034850224851 1109 21 0.5615245284130097 1109 22 0.5657904633445412 1109 23 0.7160477181954263 1109 24 0.5060033644777846 1109 25 0.5409000211206489 1110 1 1.3391507895549637 1110 2 4.0510772492344564 1110 3 2.5189919380212658 1110 4 1.6360707367535738 1110 5 1.1628115364270002 1110 6 1.027506655531127 1110 7 0.8732759020943774 1110 8 0.8038406723948341 1110 9 0.7830168500460033 1110 10 0.7735650957285927 1110 11 0.8213401190810856 1110 12 0.8891002365425535 1110 13 0.740489237097421 1110 14 0.8049868769445041 1110 15 0.9027955214948022 1110 16 1.005595841253104 1110 17 1.0312266730215711 1110 18 0.9501093321873171 1110 19 1.0790252244171867 1110 20 0.9870134129743704 1110 21 0.9093503843231545 1110 22 0.8897402177815223 1110 23 0.9245291811367196 1110 24 0.9873759666380622 1110 25 0.9179202964105571 1111 1 0.22616095143872528 1111 2 2.9028469612843644 1111 3 1.1749674671954509 1111 4 0.19772561453202542 1111 5 -0.16030688170889817 1111 6 -0.5509687331097552 1111 7 -0.7408949461010153 1111 8 -0.5563870233786687 1111 9 -0.814363184891107 1111 10 -0.9087066012879674 1111 11 -0.7738401718917984 1111 12 -0.938338652682198 1111 13 -0.8581487035010014 1111 14 -1.0891613162214426 1111 15 -1.1025489866227218 1111 16 -0.9475116418007019 1111 17 -1.201437085401146 1111 18 -1.0836685106423545 1111 19 -1.0192239957555458 1111 20 -0.9194508008823234 1111 21 -0.8681191040401615 1111 22 -0.9839601143013326 1111 23 -1.1493361847748815 1111 24 -0.9487243348986851 1111 25 -1.0205929354630285 1112 1 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-1.2443057316018198 2315 11 -1.2153415676634367 2315 12 -1.3733083572580043 2315 13 -1.2197739718860134 2315 14 -1.5733487894394713 2315 15 -1.5526466171463085 2315 16 -1.6989105226959893 2315 17 -1.648941034151619 2315 18 -1.3359328943263549 2315 19 -1.4358841612582225 2315 20 -1.323387905795279 2315 21 -1.558012451031587 2315 22 -1.4461713919553034 2315 23 -1.4703387886323895 2315 24 -1.3928397639133223 2315 25 -1.572068021306663 2316 1 -0.3886667753755155 2316 2 2.249860169411928 2316 3 0.6604454421240895 2316 4 -0.2674823318056312 2316 5 -0.49722859392480956 2316 6 -0.9430121418034147 2316 7 -1.2053374457969852 2316 8 -1.2159778790335523 2316 9 -1.3417416136032259 2316 10 -1.4599068740826553 2316 11 -1.6337598596086451 2316 12 -1.4144118875646257 2316 13 -1.5591642914284094 2316 14 -1.740644637353732 2316 15 -1.842151669739735 2316 16 -1.8789827062484505 2316 17 -1.886738363768572 2316 18 -1.851617757476241 2316 19 -1.9030442183312104 2316 20 -1.7482256331875092 2316 21 -2.0471096206093335 2316 22 -1.8774247261054242 2316 23 -2.038000659227805 2316 24 -2.0136859552609963 2316 25 -2.091780665424899 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19258.0 3 1.0 4 157.0 5 812.0 6 997.0 7 881.0 8 811.0 9 759.0 10 718.0 11 677.0 12 721.0 13 686.0 14 725.0 15 696.0 16 716.0 17 828.0 18 925.0 19 1179.0 20 1538.0 21 1782.0 22 2321.0 23 2647.0 24 3137.0 25 3532.0 26 4160.0 27 5075.0 28 5872.0 29 7359.0 30 9532.0 31 13034.0 32 18155.0 33 26422.0 34 40063.0 35 64163.0 36 114290.0 37 152133.0 38 413834.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.089472472181065 19.259914229477427 13.562626820016598 25.087986478324915 2 16.536195854991714 21.5863418467762 39.22369393853013 22.65376835970195 3 17.794559176881066 24.70258397820542 30.821228856088883 26.68162798882463 4 12.007004158175791 17.28934299084506 39.12910766503439 31.574545185944757 5 12.938574575601022 37.427275373779594 36.36024922984675 13.27390082077263 6 30.62494297172701 37.849501844457286 18.513115416534507 13.012439767281196 7 26.40050575931245 32.54120156941807 23.146852691082735 17.91143998018675 8 24.46404285919122 35.8253783418568 21.5651599615901 18.14541883736188 9 25.322834337755108 15.761311150602436 21.146626750496416 37.76922776114604 10 14.291393836167005 29.095173126974267 35.101825339236754 21.511607697621976 11 33.07346545064285 23.37844179205645 24.818704404537932 18.729388352762776 12 21.93557217280979 25.565394592198963 32.097901794055154 20.40113144093609 13 27.677504573124367 22.43568297059731 26.628510225984037 23.258302230294287 14 20.714189503321762 22.029750292202007 28.877813937927172 28.378246266549063 15 22.57700446232658 29.64807581175673 25.11655492746004 22.658364798456653 16 22.034312554041076 28.60255747363664 27.29079856962229 22.07233140269999 17 21.00411038066644 28.266905352619393 28.647311089772277 22.081673176941894 18 20.86365789119223 27.82295382556518 29.628740511581626 21.684647771660966 19 21.807502965470196 27.47459254656766 29.489917401335656 21.22798708662649 20 22.121104154265282 27.247782957996776 29.535648645008234 21.09546424272971 21 22.159062518330554 26.99604820365981 29.091217192377965 21.753672085631667 22 21.919384045179868 27.320177234742616 29.196045566578714 21.564393153498802 23 21.708625356837153 27.237482999995656 28.939112827888263 22.114778815278928 24 21.46766109707428 27.083817622100515 29.41828862669103 22.03023265413418 25 21.676174999674124 27.245936328204774 29.231063354609404 21.846825317511698 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 369.0 1 369.0 2 447.0 3 525.0 4 525.0 5 525.0 6 1265.5 7 2006.0 8 2006.0 9 2006.0 10 2738.0 11 3470.0 12 3470.0 13 3470.0 14 5260.5 15 7051.0 16 7051.0 17 7051.0 18 11073.0 19 15095.0 20 15095.0 21 15095.0 22 22206.0 23 29317.0 24 29317.0 25 29317.0 26 39262.0 27 49207.0 28 49207.0 29 49207.0 30 60031.5 31 70856.0 32 70856.0 33 70856.0 34 79698.0 35 88540.0 36 88540.0 37 88540.0 38 95289.0 39 102038.0 40 102038.0 41 102038.0 42 107315.5 43 112593.0 44 112593.0 45 112593.0 46 113777.5 47 114962.0 48 114962.0 49 114962.0 50 111183.0 51 107404.0 52 107404.0 53 107404.0 54 97272.5 55 87141.0 56 87141.0 57 87141.0 58 74044.0 59 60947.0 60 60947.0 61 60947.0 62 48697.5 63 36448.0 64 36448.0 65 36448.0 66 27925.5 67 19403.0 68 19403.0 69 19403.0 70 14048.5 71 8694.0 72 8694.0 73 8694.0 74 5854.5 75 3015.0 76 3015.0 77 3015.0 78 2051.0 79 1087.0 80 1087.0 81 1087.0 82 689.5 83 292.0 84 292.0 85 292.0 86 190.5 87 89.0 88 89.0 89 89.0 90 62.0 91 35.0 92 35.0 93 35.0 94 21.5 95 8.0 96 8.0 97 8.0 98 6.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.352289169190394 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 2.1725056376521298E-4 22 9.776275369434585E-4 23 8.690022550608519E-4 24 2.1725056376521298E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 920596.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.96031593105194 #Duplication Level Percentage of deduplicated Percentage of total 1 80.60526137619023 58.80985335711857 2 13.285767327557155 19.38667563210035 3 3.5010078914735594 7.663039255170505 4 1.231295441183461 3.5934281757283713 5 0.5393431400839044 1.9675322947883631 6 0.2728137520077262 1.1942746522091612 7 0.1563048186724254 0.798283426632016 8 0.0934674793259231 0.5455533456718732 9 0.06285690603321976 0.4127453750368942 >10 0.23410761113828427 2.7860264922063767 >50 0.010546177846217671 0.5181267835748581 >100 0.006324568981690252 0.8458680055460154 >500 1.5058491770194468E-4 0.09492528818867332 >1k 7.529245885097235E-4 1.3836679160279535 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4392 0.4770822380284077 No Hit TATCAACGCAGAGTACTTTTTTTTT 3030 0.3291346041042976 No Hit GGTATCAACGCAGAGTACTTTTTTT 2845 0.3090389269560154 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1168 0.12687432923888436 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1159 0.12589670170194092 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 655 0.0 14.792961 1 TGGACCG 40 0.0054402845 14.184659 5 GTATTAG 55 0.0022728853 12.583622 1 GTCCAAT 55 0.0022728853 12.583622 1 GTGGTAT 180 1.8189894E-12 11.534987 1 CCCTATA 60 0.0043601696 11.5349865 2 TTGGACT 70 0.0015486397 10.807359 4 CTAGGAC 70 0.0015486397 10.807359 3 GGTCTAC 185 2.7284841E-11 10.688791 1 AAGGCGT 80 3.930934E-4 10.6384945 6 TAAGACT 160 5.875336E-10 10.6384945 4 GTCCTAC 190 4.5474735E-11 10.407507 1 GTAATAC 100 2.5200527E-5 10.402083 3 AGACTGT 155 3.936293E-9 10.371579 6 GTCTTAT 135 3.7176142E-7 10.253322 1 GTATCAA 1310 0.0 10.113555 1 TTGGACC 75 0.0027486137 10.086868 4 CCTAGAC 75 0.0027486137 10.086868 3 AAAGGCG 85 6.871728E-4 10.0127 5 GGATAGA 90 7.7154796E-4 9.887132 1 >>END_MODULE