FastQCFastQC Report
Thu 26 May 2016
SRR1511680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511680_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920596
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59420.6454514249464477No Hit
TATCAACGCAGAGTACTTTTTTTTT42120.45752968728953847No Hit
GGTATCAACGCAGAGTACTTTTTTT41010.44547228100056924No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18260.19834976471763943No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13610.14783900864222743No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12120.13165384164171906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.014.8422811
AGCGTAC400.00515578514.2999671
GTTATAC655.446899E-513.1540723
GTGGTAT2000.012.3933061
TTACACC550.00306641812.0911164
GCGCCTT550.00307266312.08783213
CGCCACC951.0414278E-611.99694614
TACACGG658.0168125E-411.6925085
ACACGGT658.035103E-411.6893336
TAACCTA752.0717339E-411.4001955
CGTACAC752.0717339E-411.4001953
AGACTTA752.0769676E-411.3970996
GATATAC600.00572150711.1221971
GTAAAAC600.00587182411.0835233
GGTCCAC700.001447497510.8952131
GCGCCAC1053.4711666E-610.85438113
GTATGAC803.7669291E-410.6876833
TAGGACT1355.6710633E-810.5557374
TAGGCAG1106.0341627E-610.3638135
GCCTCGA750.002651409510.13075416
Produced by FastQC (version 0.11.5)