##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511680_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 920596 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.361448452958737 31.0 30.0 33.0 26.0 34.0 2 29.55842519411338 31.0 30.0 34.0 26.0 34.0 3 29.58934972561254 31.0 30.0 34.0 26.0 34.0 4 33.20089920008343 35.0 33.0 37.0 28.0 37.0 5 32.67939465302913 35.0 33.0 37.0 26.0 37.0 6 32.62659407601163 35.0 33.0 37.0 25.0 37.0 7 32.35750318272076 35.0 32.0 37.0 24.0 37.0 8 32.22601771026596 35.0 32.0 37.0 23.0 37.0 9 33.47879199996524 37.0 33.0 39.0 23.0 39.0 10 33.17371137828103 37.0 33.0 39.0 21.0 39.0 11 33.21500745169434 37.0 33.0 39.0 21.0 39.0 12 32.942513328322086 37.0 32.0 39.0 18.0 39.0 13 32.90661484516552 37.0 32.0 39.0 18.0 39.0 14 33.58554023697692 38.0 32.0 40.0 17.0 41.0 15 33.45209842319541 38.0 32.0 40.0 17.0 41.0 16 33.24432758778009 38.0 32.0 40.0 17.0 41.0 17 33.18255347622627 38.0 32.0 40.0 16.0 41.0 18 33.06258880116794 38.0 32.0 40.0 13.0 41.0 19 33.0867046999987 38.0 32.0 40.0 11.0 41.0 20 33.17479980360549 38.0 32.0 40.0 10.0 41.0 21 33.07893364733282 38.0 32.0 40.0 10.0 41.0 22 32.90619989658873 38.0 32.0 40.0 8.0 41.0 23 32.72862580328396 38.0 32.0 40.0 2.0 41.0 24 32.45817166270547 38.0 32.0 40.0 2.0 41.0 25 31.81506111258359 37.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22080555403258373 1101 2 0.2791290902644512 1101 3 0.31645056596645915 1101 4 0.4644837104442132 1101 5 0.6261368471994118 1101 6 0.46802702019835607 1101 7 0.6170531892867075 1101 8 0.2838914951932381 1101 9 0.030145882037729166 1101 10 0.36150275002331966 1101 11 0.3711192443591429 1101 12 0.49447478196001526 1101 13 0.4911165322918407 1101 14 0.19487364921671713 1101 15 0.3645891601870588 1101 16 0.4077918074053102 1101 17 0.2639565771659491 1101 18 0.3366581421810224 1101 19 0.1120919528040929 1101 20 -0.11831267038764537 1101 21 0.011556499597730863 1101 22 0.32224616425170893 1101 23 -0.057884484351625076 1101 24 -0.5087095032745701 1101 25 -0.15388933422025275 1102 1 0.76162507632203 1102 2 0.8207628919050762 1102 3 0.5910726568316846 1102 4 0.7867179445112527 1102 5 1.0068832003414627 1102 6 0.8845367080347373 1102 7 1.0143113181609138 1102 8 0.7785569214951593 1102 9 0.663667976614768 1102 10 0.9422416795359609 1102 11 1.2155269315273998 1102 12 1.3584298133248467 1102 13 1.1484325190777795 1102 14 1.213469840894824 1102 15 1.2087555793994156 1102 16 0.9582736473518594 1102 17 1.26136178669789 1102 18 1.5483255357447376 1102 19 1.6167804387548799 1102 20 1.5415029916145073 1102 21 1.1762068002547181 1102 22 1.239214029215887 1102 23 1.3849373299834014 1102 24 1.2621288564745115 1102 25 1.3287741712175567 1103 1 0.9041962682666629 1103 2 0.8898448605239757 1103 3 0.9539998141411132 1103 4 1.058032651382554 1103 5 1.2147609706722662 1103 6 1.2608729321480396 1103 7 1.2107354165692925 1103 8 1.4811123619611166 1103 9 1.409697949557433 1103 10 1.7222659611850375 1103 11 1.2357138731453787 1103 12 1.6453865509183032 1103 13 1.5496179419643639 1103 14 2.0304841458551905 1103 15 1.6203498411673252 1103 16 1.7309976376662775 1103 17 1.9257232763635201 1103 18 1.5993564903339959 1103 19 1.9435863864049026 1103 20 1.6596616482706352 1103 21 1.9643482837371025 1103 22 2.107824581803939 1103 23 1.7711701314222523 1103 24 1.9512797434542648 1103 25 1.7782430665604885 1104 1 0.443604665840315 1104 2 0.9156559972654961 1104 3 0.8175934604283199 1104 4 1.0457924878736833 1104 5 0.9695929600520756 1104 6 1.118170698359279 1104 7 0.9060095633723932 1104 8 1.0903504816091214 1104 9 1.0726942101464019 1104 10 1.0069442338268715 1104 11 1.301304719803234 1104 12 1.4671106231303384 1104 13 1.1334885281121103 1104 14 1.4999938164058477 1104 15 1.8010442281076493 1104 16 1.5013458826404147 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-1.398827961829383 2314 13 -1.3999661521723823 2314 14 -1.535715894720994 2314 15 -1.5994310474510556 2314 16 -1.3461917854756713 2314 17 -1.4724102192745399 2314 18 -1.2856235597099648 2314 19 -1.3312293301028397 2314 20 -1.2517132080220534 2314 21 -1.4896045126380884 2314 22 -1.2866502947768446 2314 23 -1.3979243434543314 2314 24 -1.3321313608941487 2314 25 -1.597127191098263 2315 1 -1.5316756208090396 2315 2 -1.779592837962852 2315 3 -1.6080318607244877 2315 4 -1.9334881988156774 2315 5 -1.9102260563323235 2315 6 -1.9289064231459783 2315 7 -1.9253046025269605 2315 8 -1.966206898266794 2315 9 -2.247410214089122 2315 10 -2.157334262988325 2315 11 -2.0696289252854108 2315 12 -2.097021652166159 2315 13 -2.006446155011375 2315 14 -2.699781333522939 2315 15 -2.5907259833962613 2315 16 -2.568616285990238 2315 17 -2.7956686741522887 2315 18 -2.774608264632068 2315 19 -2.803481716631815 2315 20 -2.389386652785884 2315 21 -2.4738587445128886 2315 22 -2.775003323628116 2315 23 -2.5818095061641486 2315 24 -2.6110944184902287 2315 25 -2.566973530570923 2316 1 -1.7267677518318862 2316 2 -1.9885448236486596 2316 3 -1.9888613589875384 2316 4 -2.004738827358807 2316 5 -2.1117771859232377 2316 6 -2.4865843487111583 2316 7 -2.4623956666217666 2316 8 -3.39930566057922 2316 9 -2.9139071422042093 2316 10 -2.736440085403512 2316 11 -2.798269306724947 2316 12 -2.75905050006255 2316 13 -2.773004597398536 2316 14 -3.1197515207968856 2316 15 -2.851443122395544 2316 16 -2.674857304523645 2316 17 -2.7672534196337963 2316 18 -2.5927787127673696 2316 19 -2.627432209128859 2316 20 -2.9105835520581778 2316 21 -2.7394510810087205 2316 22 -2.919015636692368 2316 23 -3.07665098567173 2316 24 -3.3615902337330077 2316 25 -3.3209777012896176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32422.0 3 1.0 4 575.0 5 4346.0 6 5301.0 7 4129.0 8 3386.0 9 3074.0 10 2905.0 11 2758.0 12 2710.0 13 2504.0 14 2451.0 15 2339.0 16 2541.0 17 2550.0 18 2682.0 19 3126.0 20 3826.0 21 4764.0 22 6115.0 23 7584.0 24 8850.0 25 10851.0 26 11763.0 27 13611.0 28 15561.0 29 19137.0 30 23633.0 31 29805.0 32 37407.0 33 48391.0 34 62704.0 35 84519.0 36 120638.0 37 173376.0 38 158261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.72332769994003 21.8926020825383 14.99689254756583 22.38717766995584 2 16.939243707337422 23.257324602757347 37.92477916480193 21.878652525103305 3 19.42567640963028 26.500223768080676 31.28918657043915 22.78491325184989 4 14.429891070567328 19.684747706920298 37.48300014338537 28.402361079126997 5 13.250920059414053 38.465057300659225 35.81815475252657 12.465867887400158 6 30.699568540380362 39.20373323368774 17.721562987455954 12.375135238475945 7 26.659685681884344 33.14906864683314 22.928733125062458 17.26251254622006 8 24.97382130706629 36.63691782280175 20.928833060321793 17.460427809810167 9 26.087990823336188 15.655836001894425 20.700937218932083 37.555235955837304 10 14.708189042750567 30.001216603157083 34.79126565833439 20.499328695757963 11 34.075316425446125 23.54137971488036 24.242012783023174 18.141291076650344 12 22.33965822141308 26.358793651069522 31.77593645855511 19.52561166896228 13 28.44450768849745 22.738964757613548 26.328704447988045 22.487823105900958 14 21.1717191906113 22.405485142233942 28.835232827429184 27.58756283972557 15 23.248634580206737 30.148186609544254 24.848793607619413 21.7543852026296 16 22.418411550777975 29.123741576109392 27.112761732616697 21.34508514049594 17 21.350625029871953 28.692933708162972 28.48448179223025 21.471959469734824 18 21.393749266779345 28.054434301257015 29.575296872895386 20.976519559068254 19 22.29827198901581 27.70802827733338 29.62841463573598 20.36528509791483 20 22.53735623443943 27.46036263464104 29.620376364876666 20.38190476604287 21 22.63272923193236 27.149694328456782 29.293957392819436 20.923619046791426 22 22.451325011188402 27.581914325067675 29.24594501822732 20.7208156455166 23 22.276655557921174 27.374440036671892 29.047595253509684 21.30130915189725 24 22.010414992026906 27.320670522140006 29.447444916119558 21.221469569713534 25 22.25675363005239 27.36777614957543 29.485899634903685 20.88957058546849 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 396.0 1 396.0 2 521.0 3 646.0 4 646.0 5 646.0 6 1433.0 7 2220.0 8 2220.0 9 2220.0 10 3101.0 11 3982.0 12 3982.0 13 3982.0 14 5686.5 15 7391.0 16 7391.0 17 7391.0 18 11822.0 19 16253.0 20 16253.0 21 16253.0 22 23750.5 23 31248.0 24 31248.0 25 31248.0 26 41767.5 27 52287.0 28 52287.0 29 52287.0 30 63228.5 31 74170.0 32 74170.0 33 74170.0 34 82866.5 35 91563.0 36 91563.0 37 91563.0 38 97238.5 39 102914.0 40 102914.0 41 102914.0 42 107581.0 43 112248.0 44 112248.0 45 112248.0 46 112482.5 47 112717.0 48 112717.0 49 112717.0 50 108600.5 51 104484.0 52 104484.0 53 104484.0 54 94265.5 55 84047.0 56 84047.0 57 84047.0 58 71144.5 59 58242.0 60 58242.0 61 58242.0 62 46692.5 63 35143.0 64 35143.0 65 35143.0 66 26739.5 67 18336.0 68 18336.0 69 18336.0 70 13241.5 71 8147.0 72 8147.0 73 8147.0 74 5502.0 75 2857.0 76 2857.0 77 2857.0 78 1913.5 79 970.0 80 970.0 81 970.0 82 617.0 83 264.0 84 264.0 85 264.0 86 159.0 87 54.0 88 54.0 89 54.0 90 33.0 91 12.0 92 12.0 93 12.0 94 8.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.374322721367462 2 0.0 3 0.0 4 0.0 5 0.029871952517716783 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0018466297920043102 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 920596.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.92972555649312 #Duplication Level Percentage of deduplicated Percentage of total 1 82.46676156514592 60.96745050043994 2 11.899557261653063 17.594620051955715 3 3.1881410576274813 7.070951802773622 4 1.137846107692333 3.364826018688724 5 0.5195111483014017 1.9203658308730613 6 0.2580218326948677 1.1445289967228975 7 0.1371471841788734 0.7097477581032949 8 0.08092650382444827 0.47862993743903615 9 0.06714343891705905 0.4467506410851602 >10 0.22749015392825767 2.7668587926764387 >50 0.010708918515689891 0.5417845969916777 >100 0.00527856092302015 0.705580169265319 >500 5.865066390218623E-4 0.265798484891478 >1k 7.33133298777328E-4 1.3779895350491305 >5k 1.4662665975546558E-4 0.6441168830445196 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5942 0.6454514249464477 No Hit TATCAACGCAGAGTACTTTTTTTTT 4212 0.45752968728953847 No Hit GGTATCAACGCAGAGTACTTTTTTT 4101 0.44547228100056924 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1826 0.19834976471763943 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1361 0.14783900864222743 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1212 0.13165384164171906 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 835 0.0 14.842281 1 AGCGTAC 40 0.005155785 14.299967 1 GTTATAC 65 5.446899E-5 13.154072 3 GTGGTAT 200 0.0 12.393306 1 TTACACC 55 0.003066418 12.091116 4 GCGCCTT 55 0.003072663 12.087832 13 CGCCACC 95 1.0414278E-6 11.996946 14 TACACGG 65 8.0168125E-4 11.692508 5 ACACGGT 65 8.035103E-4 11.689333 6 TAACCTA 75 2.0717339E-4 11.400195 5 CGTACAC 75 2.0717339E-4 11.400195 3 AGACTTA 75 2.0769676E-4 11.397099 6 GATATAC 60 0.005721507 11.122197 1 GTAAAAC 60 0.005871824 11.083523 3 GGTCCAC 70 0.0014474975 10.895213 1 GCGCCAC 105 3.4711666E-6 10.854381 13 GTATGAC 80 3.7669291E-4 10.687683 3 TAGGACT 135 5.6710633E-8 10.555737 4 TAGGCAG 110 6.0341627E-6 10.363813 5 GCCTCGA 75 0.0026514095 10.130754 16 >>END_MODULE