FastQCFastQC Report
Thu 26 May 2016
SRR1511679_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511679_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936647
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT54190.5785530728225254No Hit
CTGTAGGACGTGGAATATGGCAAGA51120.545776583921157No Hit
GTCCTAAAGTGTGTATTTCTCATTT47480.5069145579924988No Hit
CTTTAGGACGTGAAATATGGCGAGG35770.3818941394143151No Hit
GTATCAACGCAGAGTACTTTTTTTT35610.38018591849437405No Hit
GTCCTACAGTGTGCATTTCTCATTT28280.3019280475995759No Hit
TATCAACGCAGAGTACTTTTTTTTT21880.233599210801935No Hit
CTGAAGGACCTGGAATATGGCGAGA20510.21897256917493996No Hit
GGTATCAACGCAGAGTACTTTTTTT19750.2108585198052201No Hit
CTGTAGGACCTGGAATATGGCGAGA19100.2039188723179597No Hit
GTCCTTCAGTGTGCATTTCTCATTT18600.198580681943144No Hit
TTTCTAAATTTTCCACCTTTTTCAG16260.17359795098900654No Hit
ATTTAGAAATGTCCACTGTAGGACG16210.17306413195152495No Hit
GAATATGGCAAGAAAACTGAAAATC14420.15395341040968477No Hit
GGAATATGGCGAGAAAACTGAAAAT12840.13708472882526715No Hit
GATATACACTGTTCTACAAATCCCG11670.12459336334819841No Hit
GTGTATATCAATGAGTTACAATGAA11490.12267161481326477No Hit
ATTCCAGGTCCTTCAGTGTGCATTT11170.11925517297338273No Hit
TTCCAGGTCCTTCAGTGTGCATTTC10600.11316963594609282No Hit
TTGTAGAACAGTGTATATCAATGAG10380.11082083218117392No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10310.11007348552869972No Hit
ACCTGGAATATGGCGAGAAAACTGA9950.10622998845883241No Hit
CCACTGTAGGACGTGGAATATGGCA9740.10398794850140981No Hit
GCCATATTCCACGTCCTACAGTGGA9670.10324060184893562No Hit
CTGTAGGACATGGAATATGGCAAGA9630.10281354661895035No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9580.10227972758146879No Hit
GACCTGGAATATGGCGAGAAAACTG9510.10153238092899458No Hit
ACAGTGGACATTTCTAAATTTTCCA9480.10121208950650566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1400.015.53741919
TCCAACG3200.015.36853418
CCAACGT1900.014.43522219
AGGACCG602.6767506E-514.1863395
CGGTTTC2250.013.87108613
TAGGACC7950.013.7997094
CTAGAGT1650.013.756454
CGGTAGG759.896732E-613.1689811
GGCCTAA759.896732E-613.1689811
CCGGTTT2300.013.15834412
GGTATCA4900.013.1017911
GCAATTC655.681666E-513.09508113
ACTGTTC5300.012.8480058
GCCTAAA852.548055E-612.7816572
GATATAC5650.012.7610921
GGCCTAC1257.6033757E-1012.64222051
TGCCGGT2250.012.61007810
GGCGAGG9550.012.57706919
GCCGGTT2350.012.47592911
TGGCTAG1155.677066E-912.33594818
Produced by FastQC (version 0.11.5)