##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511679_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 936647 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.392662337038395 31.0 31.0 34.0 25.0 34.0 2 27.74572918079063 31.0 28.0 34.0 10.0 34.0 3 29.429211858896682 31.0 30.0 34.0 16.0 34.0 4 33.93120674063975 37.0 35.0 37.0 30.0 37.0 5 34.38136138801491 37.0 35.0 37.0 32.0 37.0 6 34.654520860046524 37.0 35.0 37.0 32.0 37.0 7 34.7271853750666 37.0 35.0 37.0 32.0 37.0 8 34.75471655810567 37.0 35.0 37.0 32.0 37.0 9 36.474491457293944 39.0 37.0 39.0 33.0 39.0 10 36.35610320643743 39.0 37.0 39.0 32.0 39.0 11 36.370641234104205 39.0 37.0 39.0 33.0 39.0 12 36.30968657349033 39.0 37.0 39.0 32.0 39.0 13 36.322539868274816 39.0 37.0 39.0 32.0 39.0 14 37.600315807342575 40.0 38.0 41.0 33.0 41.0 15 37.578423888615454 40.0 38.0 41.0 33.0 41.0 16 37.54353561160181 40.0 38.0 41.0 33.0 41.0 17 37.54325375515002 40.0 38.0 41.0 33.0 41.0 18 37.512206839930094 40.0 38.0 41.0 33.0 41.0 19 37.53755150019164 40.0 38.0 41.0 32.0 41.0 20 37.50198527300039 40.0 38.0 41.0 32.0 41.0 21 37.42210565986973 40.0 38.0 41.0 32.0 41.0 22 37.352929118440564 40.0 38.0 41.0 32.0 41.0 23 37.26504755793805 40.0 38.0 41.0 32.0 41.0 24 37.21637393810048 40.0 38.0 41.0 32.0 41.0 25 37.14466389151943 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.648945915740839 1101 2 3.3631967550717228 1101 3 1.8315609086137137 1101 4 0.7881541795626603 1101 5 0.38141157776816215 1101 6 0.073143759875272 1101 7 0.15397665963496365 1101 8 -0.2562183289209514 1101 9 -0.15795967779121867 1101 10 0.0028368153451054923 1101 11 -0.11639901388774376 1101 12 -0.17137905606758608 1101 13 -0.24940626696552215 1101 14 -0.0691192089186572 1101 15 -0.17153404385462068 1101 16 -0.1473563048638198 1101 17 -0.06492493722277004 1101 18 -0.1242958529858953 1101 19 -0.02085170493377575 1101 20 -0.00917127677673335 1101 21 -0.024490957734386143 1101 22 -0.08113589555093625 1101 23 -0.005060615144799385 1101 24 0.09036030470148404 1101 25 0.16867908175567692 1102 1 1.4873350930257239 1102 2 4.2196510574155965 1102 3 2.6610842686892227 1102 4 1.5907277205411035 1102 5 1.0581081327469235 1102 6 0.7877575714216007 1102 7 0.7709783900109244 1102 8 0.7515369015336688 1102 9 0.8820403222087805 1102 10 0.8862629597528482 1102 11 0.8470963455585405 1102 12 0.943826229444447 1102 13 1.0694925846940677 1102 14 1.031566649190509 1102 15 1.0348966624533844 1102 16 1.126621986783185 1102 17 1.2169438688178147 1102 18 1.1323314869543282 1102 19 1.0543724574069486 1102 20 1.0477266310414208 1102 21 1.1134546140762183 1102 22 0.8864274204786327 1102 23 1.0300188248426139 1102 24 0.8461602103173433 1102 25 0.8497896685104323 1103 1 1.3705859001219416 1103 2 4.008585275181055 1103 3 2.5783421138720755 1103 4 1.3425261696706912 1103 5 1.0247592284254594 1103 6 0.7177877791385825 1103 7 0.7056778180366265 1103 8 0.5968598957308799 1103 9 0.6969303391292527 1103 10 0.653228589391702 1103 11 0.6558462028244776 1103 12 0.7107555267776604 1103 13 0.6801472928418448 1103 14 0.8556549678352141 1103 15 0.7912044697468374 1103 16 0.6744737094535012 1103 17 0.9285359504513124 1103 18 0.8935773736182995 1103 19 0.7803978069756354 1103 20 0.7791405805706617 1103 21 0.6906047080211764 1103 22 0.8818993771290025 1103 23 0.7590940119539908 1103 24 0.8357826657583658 1103 25 0.7890721573771771 1104 1 1.3950103711630923 1104 2 4.0800414190723515 1104 3 2.526308811068038 1104 4 1.5249878657541416 1104 5 1.0690960441682265 1104 6 0.9506000446710061 1104 7 0.8612706296812291 1104 8 0.8479297146766385 1104 9 0.9044664180293722 1104 10 0.8601064773854148 1104 11 0.8862482063741552 1104 12 0.9716806161664024 1104 13 0.8767569887390181 1104 14 1.0633390362001336 1104 15 1.1880770280500315 1104 16 1.1053465885730205 1104 17 1.0353150063199266 1104 18 1.1111405149275342 1104 19 1.1709306207935057 1104 20 1.0319014120023766 1104 21 1.1384260029807365 1104 22 1.1467477396721435 1104 23 1.1443944150441752 1104 24 0.9337289238499196 1104 25 0.8182736869466112 1105 1 1.4822226185880965 1105 2 4.084681732262222 1105 3 2.492372612247504 1105 4 1.5876602358785306 1105 5 1.1992124272254543 1105 6 0.8521747493357097 1105 7 0.9141276742645061 1105 8 0.7658518299590256 1105 9 0.8605679295707418 1105 10 0.6071218554583666 1105 11 0.8235789631454509 1105 12 0.6442352273994629 1105 13 0.7228668178229043 1105 14 0.6880083669819186 1105 15 0.8222177258480698 1105 16 0.7237590010981094 1105 17 0.7997659547073823 1105 18 0.7969531638459415 1105 19 0.842716015689156 1105 20 0.8943523979125843 1105 21 0.9130456161212024 1105 22 0.8516625943027663 1105 23 0.9707141213661288 1105 24 0.924892316656809 1105 25 0.8722845560359929 1106 1 1.0948059853195033 1106 2 3.819895634082158 1106 3 2.2528262094770035 1106 4 1.1973127931433822 1106 5 0.657185318265519 1106 6 0.6499766422357922 1106 7 0.5618673953648923 1106 8 0.3925898143595532 1106 9 0.3728275918905197 1106 10 0.4975823378293214 1106 11 0.5140764403063045 1106 12 0.5600833843395776 1106 13 0.7447852032058506 1106 14 0.6834487591266978 1106 15 0.8702698473965071 1106 16 0.5501362862971106 1106 17 0.5090629509359701 1106 18 0.5241566660193868 1106 19 0.5926774794496765 1106 20 0.6470843603834311 1106 21 0.4918291041133145 1106 22 0.39743645337316735 1106 23 0.5460797018246595 1106 24 0.5129553311156059 1106 25 0.535688487760055 1107 1 1.4613354411090889 1107 2 4.124391517755846 1107 3 2.5586585627326457 1107 4 1.6597526520121946 1107 5 1.2309424015543087 1107 6 1.0078136998643572 1107 7 0.8656896983692803 1107 8 0.8570931822629007 1107 9 0.9923240101520392 1107 10 0.9717457078710225 1107 11 1.0061378109895074 1107 12 0.9327071414228669 1107 13 1.045661927251608 1107 14 1.0668818821888166 1107 15 1.163528694825139 1107 16 1.1039639351798272 1107 17 1.2058661156959403 1107 18 1.0128127122077757 1107 19 1.2235018138878289 1107 20 1.3342275230050475 1107 21 1.261368289401453 1107 22 1.2884767612957617 1107 23 1.4354467498306462 1107 24 1.3038245709317096 1107 25 1.3135672432395822 1108 1 1.4136040353989614 1108 2 4.117512994387962 1108 3 2.5756207376735425 1108 4 1.5339661453746203 1108 5 1.1158987572553443 1108 6 0.928271965003475 1108 7 0.6651305057888095 1108 8 0.6683543206517015 1108 9 0.5893052794737343 1108 10 0.7112983538066473 1108 11 0.6625753450866156 1108 12 0.7135354738469459 1108 13 0.6549954424361886 1108 14 0.5333166885172389 1108 15 0.624876212555634 1108 16 0.8597377122301992 1108 17 0.9140494217515851 1108 18 0.8236101299200911 1108 19 0.7938918848098098 1108 20 0.8707432531377961 1108 21 0.7812355379921101 1108 22 0.940658976243931 1108 23 0.867802632718444 1108 24 0.9330526123937943 1108 25 0.8955594236360156 1109 1 0.7706712931671511 1109 2 2.94216951647439 1109 3 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-1.5200817966429412 2315 9 -1.5938439221642113 2315 10 -1.7430053810437087 2315 11 -1.6257006875624072 2315 12 -1.7773713060469731 2315 13 -1.856440647376104 2315 14 -1.8007277504307382 2315 15 -1.7229265741539024 2315 16 -1.9654814594519934 2315 17 -1.8595098164378783 2315 18 -2.09722354694253 2315 19 -1.9147094013638224 2315 20 -1.8945369293449588 2315 21 -2.0403009997823744 2315 22 -2.061711375804876 2315 23 -1.857480435410281 2315 24 -1.842557495611814 2315 25 -1.6649395182937923 2316 1 -0.5451044878170563 2316 2 1.9138375061835653 2316 3 0.5038565216373776 2316 4 -0.2327572573230796 2316 5 -0.6454187082912455 2316 6 -0.7332747324104574 2316 7 -1.320161593261318 2316 8 -1.0834735861547955 2316 9 -1.368851897634194 2316 10 -1.3828239763970558 2316 11 -1.2570974161278983 2316 12 -1.548788598701968 2316 13 -1.4768174567749952 2316 14 -1.8422460963067877 2316 15 -1.571769040834802 2316 16 -1.9542733421448801 2316 17 -1.8088133685630226 2316 18 -1.984125644892778 2316 19 -1.827539235818314 2316 20 -1.5653783815919766 2316 21 -1.7965614568284423 2316 22 -1.7365084316515293 2316 23 -2.170519353408679 2316 24 -1.8086634695257118 2316 25 -1.9523674234878357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22039.0 3 0.0 4 185.0 5 989.0 6 1144.0 7 958.0 8 929.0 9 875.0 10 814.0 11 754.0 12 784.0 13 733.0 14 743.0 15 814.0 16 793.0 17 856.0 18 1000.0 19 1230.0 20 1510.0 21 1928.0 22 2329.0 23 2655.0 24 3147.0 25 3603.0 26 4154.0 27 4856.0 28 5677.0 29 7075.0 30 9108.0 31 12276.0 32 17098.0 33 25013.0 34 38029.0 35 61283.0 36 112841.0 37 153112.0 38 435313.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.87687250372873 19.2353149051884 13.242235335190312 26.64557725589256 2 14.082867550399888 20.697932938407384 44.461169145824904 20.758030365367826 3 18.493413206896513 24.83155340272269 30.088496520033697 26.5865368703471 4 11.030623062904167 16.940106571632644 40.1044363564929 31.924834008970294 5 11.295183777880034 38.68127480256703 37.788195552860365 12.235345866692574 6 29.628237745917087 39.1686515837877 18.853954584811568 12.349156085483646 7 26.117736991630785 31.34895003133518 23.824450406609962 18.708862570424074 8 21.93078075304784 41.91974137535272 21.052755200198153 15.096722671401286 9 25.329286273270508 15.230390958386671 21.4366778519549 38.00364491638793 10 14.063996361489439 29.373819592653366 36.46880841982092 20.093375626036273 11 32.91165188165873 21.515362778079684 27.88670651803721 17.68627882222438 12 23.563519661088968 23.56085056590156 34.753434324777636 18.12219544823183 13 29.048296743597106 22.931050865480806 26.650061335807408 21.37059105511468 14 18.13500710513139 24.274780146629414 28.627754105869123 28.96245864237007 15 20.187541304248025 36.0549919019652 23.505440149810973 20.2520266439758 16 18.615978057902282 28.224934260185535 33.08439572218776 20.074691959724422 17 18.81765489026282 30.260065958680272 31.07200471468974 19.85027443636717 18 18.706513766659157 27.134555494225683 35.658577884731386 18.500352854383774 19 22.41335316293118 26.11175821841099 28.997477171228862 22.477411447428967 20 21.987472334828382 28.336395675211683 30.95958242539612 18.716549564563813 21 19.811326797957822 27.289819205975363 28.680526047193922 24.218327948872894 22 21.04219671014491 30.940908804584915 29.199465748203412 18.81742873706676 23 18.8807060099675 30.087738778020253 30.921460021096596 20.110095190915654 24 22.07352396367041 26.89326928928401 32.003945990325064 19.029260756720515 25 20.143341087944552 28.75736536816965 31.575502830842357 19.52379071304344 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 139.0 1 139.0 2 235.0 3 331.0 4 331.0 5 331.0 6 955.0 7 1579.0 8 1579.0 9 1579.0 10 2168.5 11 2758.0 12 2758.0 13 2758.0 14 4030.5 15 5303.0 16 5303.0 17 5303.0 18 9329.5 19 13356.0 20 13356.0 21 13356.0 22 22213.5 23 31071.0 24 31071.0 25 31071.0 26 46421.5 27 61772.0 28 61772.0 29 61772.0 30 82152.0 31 102532.0 32 102532.0 33 102532.0 34 109773.0 35 117014.0 36 117014.0 37 117014.0 38 118519.0 39 120024.0 40 120024.0 41 120024.0 42 120146.5 43 120269.0 44 120269.0 45 120269.0 46 118700.5 47 117132.0 48 117132.0 49 117132.0 50 105665.0 51 94198.0 52 94198.0 53 94198.0 54 78099.5 55 62001.0 56 62001.0 57 62001.0 58 51531.0 59 41061.0 60 41061.0 61 41061.0 62 32871.0 63 24681.0 64 24681.0 65 24681.0 66 18657.0 67 12633.0 68 12633.0 69 12633.0 70 9150.0 71 5667.0 72 5667.0 73 5667.0 74 3892.0 75 2117.0 76 2117.0 77 2117.0 78 1421.5 79 726.0 80 726.0 81 726.0 82 466.0 83 206.0 84 206.0 85 206.0 86 128.5 87 51.0 88 51.0 89 51.0 90 35.5 91 20.0 92 20.0 93 20.0 94 12.0 95 4.0 96 4.0 97 4.0 98 3.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.2459966241284075 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0676380749631398E-4 22 0.0010676380749631397 23 3.2029142248894194E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 936647.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.02528632297398 #Duplication Level Percentage of deduplicated Percentage of total 1 88.88645292998991 62.242993166560936 2 7.6292465200711135 10.684803439930652 3 1.6336280767922269 3.4318582146787504 4 0.629583118165705 1.7634695245465701 5 0.3195825809646419 1.1189430867942034 6 0.18208728019114576 0.7650428358693941 7 0.12481013865461554 0.6117905986709676 8 0.09771751924724009 0.5474157811246967 9 0.06531679979304642 0.4116444846487599 >10 0.35575854045643324 4.606151922922047 >50 0.037601650089028694 1.8728855574581413 >100 0.030324331206580162 4.330578567188001 >500 0.004641479046074495 2.4376567437258805 >1k 0.0029396033958471804 4.033834379517587 >5k 3.0943193640496636E-4 1.140931696363433 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5419 0.5785530728225254 No Hit CTGTAGGACGTGGAATATGGCAAGA 5112 0.545776583921157 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4748 0.5069145579924988 No Hit CTTTAGGACGTGAAATATGGCGAGG 3577 0.3818941394143151 No Hit GTATCAACGCAGAGTACTTTTTTTT 3561 0.38018591849437405 No Hit GTCCTACAGTGTGCATTTCTCATTT 2828 0.3019280475995759 No Hit TATCAACGCAGAGTACTTTTTTTTT 2188 0.233599210801935 No Hit CTGAAGGACCTGGAATATGGCGAGA 2051 0.21897256917493996 No Hit GGTATCAACGCAGAGTACTTTTTTT 1975 0.2108585198052201 No Hit CTGTAGGACCTGGAATATGGCGAGA 1910 0.2039188723179597 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1860 0.198580681943144 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1626 0.17359795098900654 No Hit ATTTAGAAATGTCCACTGTAGGACG 1621 0.17306413195152495 No Hit GAATATGGCAAGAAAACTGAAAATC 1442 0.15395341040968477 No Hit GGAATATGGCGAGAAAACTGAAAAT 1284 0.13708472882526715 No Hit GATATACACTGTTCTACAAATCCCG 1167 0.12459336334819841 No Hit GTGTATATCAATGAGTTACAATGAA 1149 0.12267161481326477 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1117 0.11925517297338273 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 1060 0.11316963594609282 No Hit TTGTAGAACAGTGTATATCAATGAG 1038 0.11082083218117392 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1031 0.11007348552869972 No Hit ACCTGGAATATGGCGAGAAAACTGA 995 0.10622998845883241 No Hit CCACTGTAGGACGTGGAATATGGCA 974 0.10398794850140981 No Hit GCCATATTCCACGTCCTACAGTGGA 967 0.10324060184893562 No Hit CTGTAGGACATGGAATATGGCAAGA 963 0.10281354661895035 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 958 0.10227972758146879 No Hit GACCTGGAATATGGCGAGAAAACTG 951 0.10153238092899458 No Hit ACAGTGGACATTTCTAAATTTTCCA 948 0.10121208950650566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 140 0.0 15.537419 19 TCCAACG 320 0.0 15.368534 18 CCAACGT 190 0.0 14.435222 19 AGGACCG 60 2.6767506E-5 14.186339 5 CGGTTTC 225 0.0 13.871086 13 TAGGACC 795 0.0 13.799709 4 CTAGAGT 165 0.0 13.75645 4 CGGTAGG 75 9.896732E-6 13.168981 1 GGCCTAA 75 9.896732E-6 13.168981 1 CCGGTTT 230 0.0 13.158344 12 GGTATCA 490 0.0 13.101791 1 GCAATTC 65 5.681666E-5 13.095081 13 ACTGTTC 530 0.0 12.848005 8 GCCTAAA 85 2.548055E-6 12.781657 2 GATATAC 565 0.0 12.761092 1 GGCCTAC 125 7.6033757E-10 12.6422205 1 TGCCGGT 225 0.0 12.610078 10 GGCGAGG 955 0.0 12.577069 19 GCCGGTT 235 0.0 12.475929 11 TGGCTAG 115 5.677066E-9 12.335948 18 >>END_MODULE