FastQCFastQC Report
Thu 26 May 2016
SRR1511679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511679_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936647
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57440.6132513102588275No Hit
CTGTAGGACGTGGAATATGGCAAGA44850.4788356766209682No Hit
TATCAACGCAGAGTACTTTTTTTTT41420.44221569064973254No Hit
GTCCTACAGTGGACATTTCTAAATT38860.41488415593067607No Hit
GGTATCAACGCAGAGTACTTTTTTT37990.4055957046784968No Hit
GTCCTAAAGTGTGTATTTCTCATTT35270.3765559490394994No Hit
CTTTAGGACGTGAAATATGGCGAGG32460.34655531913303517No Hit
CTGAAGGACCTGGAATATGGCGAGA24460.26114427313598404No Hit
TTTCTAAATTTTCCACCTTTTTCAG20390.2176914034849842No Hit
CTGTAGGACCTGGAATATGGCGAGA20260.2163034739875321No Hit
GTCCTACAGTGTGCATTTCTCATTT20070.21427496164510215No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19570.20893677127028645No Hit
ATTTAGAAATGTCCACTGTAGGACG17620.18811782880850525No Hit
GAATATGGCAAGAAAACTGAAAATC16140.17231678529905078No Hit
TTGTAGAACAGTGTATATCAATGAG15490.16537713781179036No Hit
GGAATATGGCGAGAAAACTGAAAAT15030.16046600266695993No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14510.1549142846771516No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13700.14626641626995016No Hit
ACAGTGGACATTTCTAAATTTTCCA12990.13868618593771187No Hit
GTCCTTCAGTGTGCATTTCTCATTT12580.134308869830363No Hit
ATTCCAGGTCCTTCAGTGTGCATTT12480.13324123175539987No Hit
ACCTGGAATATGGCGAGAAAACTGA11550.12331219765824265No Hit
GAAATATGGCGAGGAAAACTGAAAA10570.11284934452360387No Hit
TTCCAGGTCCTTCAGTGTGCATTTC10360.11060730456618129No Hit
GATATACACTGTTCTACAAATCCCG10210.10900584745373657No Hit
CCACTGTAGGACGTGGAATATGGCA10190.10879231983874395No Hit
CTGTAGGACATGGAATATGGCAAGA9920.10590969703634347No Hit
GCCATATTCCACGTCCTACAGTGGA9900.10569616942135085No Hit
GACCTGGAATATGGCGAGAAAACTG9540.10185267235148354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1100.015.54139318
AGGACCG653.366602E-614.6162475
CCAACGA954.9094524E-913.99634119
GGTATCA9500.013.8474021
TAGGACC6250.013.8328164
AATGTCC6250.013.8283858
AATGGCG551.9631896E-413.81457216
AAATGTC6100.013.3899437
GAACAGT3000.013.2965256
TTAGGGT655.4449563E-513.1546234
ACTACTC655.4613498E-513.1504098
CCACCTT4750.012.99660313
TAGAAAT7300.012.6240334
CACCTTT5050.012.41259814
GAAATGT6500.012.2737156
TGTCCAC6750.012.24124410
TTCCACC5250.012.12064111
AACAGTG3550.012.0391067
ATGTCCA7000.011.9397369
ACCTTTT5450.011.85011315
Produced by FastQC (version 0.11.5)