##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511679_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 936647 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.54915245551419 31.0 28.0 31.0 23.0 34.0 2 28.730472632699406 31.0 28.0 33.0 23.0 34.0 3 28.737962113795273 31.0 28.0 33.0 23.0 34.0 4 32.19794116673624 35.0 32.0 37.0 25.0 37.0 5 31.672585296274903 35.0 32.0 37.0 20.0 37.0 6 31.71599012221253 35.0 32.0 37.0 19.0 37.0 7 31.418261095161785 35.0 32.0 37.0 17.0 37.0 8 31.25303129140434 35.0 32.0 37.0 17.0 37.0 9 32.34092032537338 37.0 32.0 39.0 17.0 39.0 10 31.952741000611756 37.0 32.0 39.0 11.0 39.0 11 32.0189676580398 37.0 32.0 39.0 11.0 39.0 12 31.707364674204904 37.0 31.0 39.0 10.0 39.0 13 31.737656769305833 37.0 31.0 39.0 10.0 39.0 14 32.18035930291775 37.0 31.0 40.0 10.0 41.0 15 32.112462859540464 37.0 31.0 40.0 10.0 41.0 16 31.858316953985867 37.0 31.0 40.0 8.0 41.0 17 31.805043949321355 37.0 31.0 40.0 2.0 41.0 18 31.651215452566444 37.0 30.0 40.0 2.0 41.0 19 31.710346587348276 37.0 30.0 40.0 2.0 41.0 20 31.80339444849554 37.0 31.0 40.0 2.0 41.0 21 31.669604450769608 37.0 31.0 40.0 2.0 41.0 22 31.482813696088282 37.0 30.0 40.0 2.0 41.0 23 31.249438689282087 37.0 30.0 40.0 2.0 41.0 24 30.95814538454722 37.0 30.0 40.0 2.0 41.0 25 30.301989970607924 36.0 29.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03658088131187398 1101 2 0.10728651697214531 1101 3 0.2536918240056494 1101 4 0.41005388930611986 1101 5 0.5099581290123929 1101 6 0.023018790519071075 1101 7 0.059991147211846396 1101 8 -0.22817949785534708 1101 9 -0.0347518841471981 1101 10 -0.0023336566635663303 1101 11 -0.13995924745133692 1101 12 0.16087056061736504 1101 13 0.14337507268197314 1101 14 -0.41028537502637974 1101 15 -0.012743336553668883 1101 16 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3 0.28109257891909323 1103 4 0.6576863245527704 1103 5 0.6521317591048081 1103 6 0.42348735748509725 1103 7 0.08648600599097023 1103 8 0.2030739187787809 1103 9 0.2920110990624849 1103 10 0.2354796974949629 1103 11 0.1690294288469829 1103 12 0.31247800562582384 1103 13 0.28987513776071694 1103 14 0.5454610781820008 1103 15 0.3454500774773024 1103 16 0.47959054074614116 1103 17 0.8882990375999462 1103 18 0.33699119805220334 1103 19 0.5151238518883865 1103 20 0.6486620659755182 1103 21 0.7952431194637306 1103 22 0.8374128641279874 1103 23 0.3559051125636792 1103 24 0.22732887699222815 1103 25 0.19330472375499852 1104 1 -0.09240210308395547 1104 2 0.04915431278489635 1104 3 0.17621358359162898 1104 4 0.23202205365960538 1104 5 0.17236854224849552 1104 6 -0.0029986171497142777 1104 7 0.24047887558948133 1104 8 0.6381515615032356 1104 9 0.38228881215955113 1104 10 0.5931278033850482 1104 11 0.46025717221462514 1104 12 0.4756513964991207 1104 13 0.5570157846713073 1104 14 0.3271724780278049 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2313 19 -0.2911820772549518 2313 20 -0.5924756178383923 2313 21 -0.4822282291154707 2313 22 -0.26265516664285116 2313 23 -0.2578423362824154 2313 24 -0.10733541731935503 2313 25 -0.11804594870300633 2314 1 -0.7755191133350934 2314 2 -0.6064450819786593 2314 3 -0.5573546938467153 2314 4 -0.8735165833666478 2314 5 -0.5669413945677135 2314 6 -0.8143346702164358 2314 7 -0.9166953256750432 2314 8 -0.8728912370556046 2314 9 -0.7765729858012875 2314 10 -1.6395136791463045 2314 11 -1.594344986972775 2314 12 -1.0189485068843744 2314 13 -1.2347192477505509 2314 14 -1.4918067068608636 2314 15 -1.0075187768405947 2314 16 -1.3697052637535059 2314 17 -1.045128259115927 2314 18 -1.169714820196475 2314 19 -0.9139055346296381 2314 20 -1.1313237335433612 2314 21 -1.4841566452745631 2314 22 -0.9988280353771124 2314 23 -0.8073548516171947 2314 24 -1.3976438347697986 2314 25 -1.4355243723949762 2315 1 -0.8522442485421955 2315 2 -0.7831809576753912 2315 3 -0.7676982328828004 2315 4 -0.8948286236792882 2315 5 -1.0814376991680028 2315 6 -1.4607309003045756 2315 7 -1.3530948450381324 2315 8 -1.4169205317410771 2315 9 -1.486861703588211 2315 10 -1.6422413159924467 2315 11 -1.2468039996980167 2315 12 -1.6982934265208982 2315 13 -1.3297983134174558 2315 14 -1.4232790266052397 2315 15 -1.603360864791778 2315 16 -1.590018438530361 2315 17 -1.6635097120804154 2315 18 -1.3027637543147605 2315 19 -1.117821561901529 2315 20 -1.289600511485979 2315 21 -1.719487485919263 2315 22 -1.5204600527767056 2315 23 -1.5379442136097374 2315 24 -1.2311539386616595 2315 25 -1.4484621871976096 2316 1 -1.0253704721169967 2316 2 -1.0540026720686484 2316 3 -0.9743145450713833 2316 4 -1.1917272560296297 2316 5 -1.146324745857207 2316 6 -1.2136967423420728 2316 7 -1.589794170967476 2316 8 -2.396398901577964 2316 9 -1.8710819780361803 2316 10 -1.9509516767591037 2316 11 -1.3536110346363124 2316 12 -1.9263528512313144 2316 13 -1.929794641258617 2316 14 -1.643563802523225 2316 15 -1.7067091851233762 2316 16 -1.8098017352568618 2316 17 -1.6476035265583597 2316 18 -1.8138789201012564 2316 19 -1.5606758850652795 2316 20 -1.7751838453930837 2316 21 -1.6669629914722535 2316 22 -2.017217077501808 2316 23 -1.6885692838971522 2316 24 -2.004922560523397 2316 25 -1.8272889783504596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44982.0 3 2.0 4 664.0 5 5142.0 6 6124.0 7 5311.0 8 4261.0 9 3810.0 10 3630.0 11 3485.0 12 3178.0 13 3072.0 14 2934.0 15 3053.0 16 3117.0 17 3300.0 18 3278.0 19 3916.0 20 4739.0 21 6130.0 22 7591.0 23 9273.0 24 11084.0 25 12720.0 26 14675.0 27 16851.0 28 19427.0 29 23229.0 30 28843.0 31 35805.0 32 44798.0 33 56090.0 34 71669.0 35 91657.0 36 117612.0 37 146965.0 38 114230.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.268379425887055 23.499969460937326 15.451694167896266 20.779956945279356 2 16.05994574263303 24.236131648315748 41.84607434818026 17.857848260870956 3 21.647216080337632 27.899838466359256 30.743172187601093 19.709773265702022 4 15.462495475883658 20.546267697435642 38.34112531188377 25.65011151479693 5 12.05377834640876 40.85845004389328 36.27913136158878 10.808640248109173 6 30.2563292254179 40.812173636385964 17.698343132471464 11.233154005724675 7 26.8472540882531 33.24528878008471 23.30856768878777 16.598889442874423 8 23.51889238955551 42.627051600015804 20.17056585885611 13.683490151572578 9 26.666609725969337 15.692251189615725 21.36279729716745 36.27834178724749 10 15.1311006174151 31.68205311072368 34.940057460281196 18.246788811580032 11 34.2823924061039 22.118898581856346 27.147153623510246 16.45155538852951 12 24.33851813970471 25.520607016303902 33.74675838389489 16.394116460096495 13 30.833707896357964 23.44768092995547 26.23859362171661 19.48001755196995 14 19.758030506690353 25.473737704813022 28.40290952728189 26.365322261214736 15 21.6886404376462 36.818673416986336 23.156429263105522 18.336256882261942 16 20.086756269971502 29.94180304853376 31.268450120482953 18.702990561011777 17 20.056648876257544 31.76308684061338 29.570905581291562 18.60935870183751 18 19.850381200174667 28.327213987767003 34.53275353468276 17.289651277375576 19 23.820606909540093 26.945690318764697 29.387271832397904 19.846430939297303 20 23.43550985590089 29.159864922430756 30.4619563186558 16.942668903012553 21 21.550488070745967 27.81314625467225 28.843096705589193 21.79326896899259 22 22.646738846118122 30.787052112482076 29.4626470804903 17.1035619609095 23 20.63402754719761 30.627653747890083 30.450746118868686 18.28757258604362 24 23.227106903668084 28.138669103728514 31.217737311922207 17.416486680681196 25 21.572636395763112 29.84398338299715 30.923063618436714 17.660316602803025 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 185.0 2 364.5 3 544.0 4 544.0 5 544.0 6 1297.5 7 2051.0 8 2051.0 9 2051.0 10 2922.0 11 3793.0 12 3793.0 13 3793.0 14 5082.5 15 6372.0 16 6372.0 17 6372.0 18 11106.0 19 15840.0 20 15840.0 21 15840.0 22 25800.5 23 35761.0 24 35761.0 25 35761.0 26 52889.0 27 70017.0 28 70017.0 29 70017.0 30 89762.0 31 109507.0 32 109507.0 33 109507.0 34 115350.5 35 121194.0 36 121194.0 37 121194.0 38 120415.5 39 119637.0 40 119637.0 41 119637.0 42 118681.0 43 117725.0 44 117725.0 45 117725.0 46 113496.0 47 109267.0 48 109267.0 49 109267.0 50 98937.0 51 88607.0 52 88607.0 53 88607.0 54 72799.0 55 56991.0 56 56991.0 57 56991.0 58 47063.0 59 37135.0 60 37135.0 61 37135.0 62 29617.5 63 22100.0 64 22100.0 65 22100.0 66 16875.5 67 11651.0 68 11651.0 69 11651.0 70 8416.0 71 5181.0 72 5181.0 73 5181.0 74 3621.0 75 2061.0 76 2061.0 77 2061.0 78 1385.0 79 709.0 80 709.0 81 709.0 82 474.5 83 240.0 84 240.0 85 240.0 86 151.5 87 63.0 88 63.0 89 63.0 90 37.0 91 11.0 92 11.0 93 11.0 94 6.5 95 2.0 96 2.0 97 2.0 98 2.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3647051664074085 2 0.0 3 0.0 4 0.0 5 0.030427685136449483 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0017082209199410236 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 936647.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.46825658690953 #Duplication Level Percentage of deduplicated Percentage of total 1 90.29583638583031 63.629901671662914 2 6.534212835885439 9.209091734253057 3 1.4535130185399485 3.072795850286594 4 0.557565275264838 1.571626115252538 5 0.2807191456114083 0.9890894390901369 6 0.17606759850845316 0.7444306025000789 7 0.12115251386666999 0.5976184503313929 8 0.09035860092586702 0.5093930459902526 9 0.06324288844817455 0.40109544814169346 >10 0.3517642936730838 4.586296014000492 >50 0.036354081735863616 1.819136421568494 >100 0.031370689574520834 4.744475025293119 >500 0.003921336067667333 1.99839525128091 >1k 0.0037705154496801277 5.516178818910907 >5k 1.508206179872051E-4 0.6104761114374062 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5744 0.6132513102588275 No Hit CTGTAGGACGTGGAATATGGCAAGA 4485 0.4788356766209682 No Hit TATCAACGCAGAGTACTTTTTTTTT 4142 0.44221569064973254 No Hit GTCCTACAGTGGACATTTCTAAATT 3886 0.41488415593067607 No Hit GGTATCAACGCAGAGTACTTTTTTT 3799 0.4055957046784968 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3527 0.3765559490394994 No Hit CTTTAGGACGTGAAATATGGCGAGG 3246 0.34655531913303517 No Hit CTGAAGGACCTGGAATATGGCGAGA 2446 0.26114427313598404 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2039 0.2176914034849842 No Hit CTGTAGGACCTGGAATATGGCGAGA 2026 0.2163034739875321 No Hit GTCCTACAGTGTGCATTTCTCATTT 2007 0.21427496164510215 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1957 0.20893677127028645 No Hit ATTTAGAAATGTCCACTGTAGGACG 1762 0.18811782880850525 No Hit GAATATGGCAAGAAAACTGAAAATC 1614 0.17231678529905078 No Hit TTGTAGAACAGTGTATATCAATGAG 1549 0.16537713781179036 No Hit GGAATATGGCGAGAAAACTGAAAAT 1503 0.16046600266695993 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1451 0.1549142846771516 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1370 0.14626641626995016 No Hit ACAGTGGACATTTCTAAATTTTCCA 1299 0.13868618593771187 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1258 0.134308869830363 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1248 0.13324123175539987 No Hit ACCTGGAATATGGCGAGAAAACTGA 1155 0.12331219765824265 No Hit GAAATATGGCGAGGAAAACTGAAAA 1057 0.11284934452360387 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 1036 0.11060730456618129 No Hit GATATACACTGTTCTACAAATCCCG 1021 0.10900584745373657 No Hit CCACTGTAGGACGTGGAATATGGCA 1019 0.10879231983874395 No Hit CTGTAGGACATGGAATATGGCAAGA 992 0.10590969703634347 No Hit GCCATATTCCACGTCCTACAGTGGA 990 0.10569616942135085 No Hit GACCTGGAATATGGCGAGAAAACTG 954 0.10185267235148354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 110 0.0 15.541393 18 AGGACCG 65 3.366602E-6 14.616247 5 CCAACGA 95 4.9094524E-9 13.996341 19 GGTATCA 950 0.0 13.847402 1 TAGGACC 625 0.0 13.832816 4 AATGTCC 625 0.0 13.828385 8 AATGGCG 55 1.9631896E-4 13.814572 16 AAATGTC 610 0.0 13.389943 7 GAACAGT 300 0.0 13.296525 6 TTAGGGT 65 5.4449563E-5 13.154623 4 ACTACTC 65 5.4613498E-5 13.150409 8 CCACCTT 475 0.0 12.996603 13 TAGAAAT 730 0.0 12.624033 4 CACCTTT 505 0.0 12.412598 14 GAAATGT 650 0.0 12.273715 6 TGTCCAC 675 0.0 12.241244 10 TTCCACC 525 0.0 12.120641 11 AACAGTG 355 0.0 12.039106 7 ATGTCCA 700 0.0 11.939736 9 ACCTTTT 545 0.0 11.850113 15 >>END_MODULE