FastQCFastQC Report
Thu 26 May 2016
SRR1511678_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511678_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853968
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38570.45165626815056303No Hit
TATCAACGCAGAGTACTTTTTTTTT23360.27354654975362075No Hit
GGTATCAACGCAGAGTACTTTTTTT22020.25785509527289074No Hit
GTCCTACAGTGGACATTTCTAAATT16690.1954405785696888No Hit
CTGTAGGACGTGGAATATGGCAAGA16270.1905223614936391No Hit
GTCCTAAAGTGTGTATTTCTCATTT14450.1692100874974238No Hit
CTTTAGGACGTGAAATATGGCGAGG13490.15796844846645308No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10200.11944241470406385No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9850.11534390047402245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCC350.002235814516.2120383
GCCCTAA551.4015469E-414.3733921
TAGGACC2050.014.3008634
CTAGGAC1257.2759576E-1213.61811353
GGTATCA4900.013.5117231
ACGAAAT655.683539E-513.09433912
CCGGTTT604.2480542E-412.609364512
TCCAACG854.1421245E-612.23850218
GCCGGTT658.327669E-411.63941311
GACCATA658.327669E-411.6394137
GTGTTAA958.769071E-611.4419771
CTACTGG700.001072211811.2933791
GTCTAAG1052.15605E-611.2933791
ACTGTTC2100.011.2583618
TATACTG1355.0586095E-911.20832355
GATATAC2350.010.9329531
TAGGACT1303.481E-810.911954
GTTTAAG1001.5492313E-510.8698771
GTCGAGG700.001547644510.80802519
CGGTTTC700.001547644510.80802513
Produced by FastQC (version 0.11.5)