##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511678_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 853968 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.323234594270513 31.0 30.0 34.0 25.0 34.0 2 27.67165514398666 31.0 28.0 34.0 10.0 34.0 3 29.35068878459146 31.0 30.0 34.0 16.0 34.0 4 33.85870313641729 37.0 35.0 37.0 28.0 37.0 5 34.311200185487046 37.0 35.0 37.0 32.0 37.0 6 34.575815487231374 37.0 35.0 37.0 32.0 37.0 7 34.652965919097674 37.0 35.0 37.0 32.0 37.0 8 34.685821951173814 37.0 35.0 37.0 32.0 37.0 9 36.39194911284732 39.0 37.0 39.0 33.0 39.0 10 36.300987859029846 39.0 37.0 39.0 32.0 39.0 11 36.31794048488936 39.0 37.0 39.0 32.0 39.0 12 36.24859713712926 39.0 37.0 39.0 32.0 39.0 13 36.24768960897832 39.0 37.0 39.0 32.0 39.0 14 37.538435866449326 40.0 38.0 41.0 32.0 41.0 15 37.49332293481723 40.0 38.0 41.0 32.0 41.0 16 37.45042554287748 40.0 38.0 41.0 32.0 41.0 17 37.43664985104828 40.0 38.0 41.0 32.0 41.0 18 37.398589876904055 40.0 38.0 41.0 32.0 41.0 19 37.44032797481873 40.0 38.0 41.0 32.0 41.0 20 37.38413148970453 40.0 38.0 41.0 32.0 41.0 21 37.31907518782905 40.0 38.0 41.0 32.0 41.0 22 37.23850893710303 40.0 38.0 41.0 32.0 41.0 23 37.158219043336516 40.0 38.0 41.0 32.0 41.0 24 37.08857006351526 40.0 38.0 41.0 32.0 41.0 25 37.002559814887675 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1046443537518158 1101 2 3.9283477681956285 1101 3 2.265324805995153 1101 4 1.3579383422902325 1101 5 0.7960102951188759 1101 6 0.5641554226193648 1101 7 0.46168670980348736 1101 8 0.23432880234216924 1101 9 0.2138555455148179 1101 10 0.47081551308315284 1101 11 0.32436743637131116 1101 12 0.3379700197919533 1101 13 0.4791941578938932 1101 14 0.3376317917806233 1101 15 0.3701241217385771 1101 16 0.5612725509727312 1101 17 0.5914300464340201 1101 18 0.7862947101810107 1101 19 0.5741350504748439 1101 20 0.7291335857354042 1101 21 0.7360803034368644 1101 22 0.8449532865414042 1101 23 0.7922059646868149 1101 24 0.8727629757973716 1101 25 0.8314739992820535 1102 1 1.602232459113349 1102 2 4.350164519109885 1102 3 2.8804912021315445 1102 4 1.7818495580292577 1102 5 1.2480074719953436 1102 6 1.0896132936776581 1102 7 0.7806950933822492 1102 8 0.8879608285510017 1102 9 1.0366187520249355 1102 10 1.0542858273901388 1102 11 0.9238522277655861 1102 12 1.0165274356959273 1102 13 0.8658911605557265 1102 14 1.0389217579100034 1102 15 1.1089897705708367 1102 16 0.9340541169369345 1102 17 1.0728343788076273 1102 18 1.0907505096523096 1102 19 1.1048937134490302 1102 20 1.1436099866875793 1102 21 0.9889454649714651 1102 22 0.8833617220528254 1102 23 0.9960222145767119 1102 24 0.9026185366529589 1102 25 0.8696788221562173 1103 1 1.47630299146752 1103 2 4.108493390998518 1103 3 2.679515183940076 1103 4 1.5222664309325253 1103 5 1.0386214902474222 1103 6 0.7358347586701868 1103 7 0.6024988189972262 1103 8 0.31275546179779923 1103 9 0.5584394255178893 1103 10 0.5084597754712732 1103 11 0.7052234794505523 1103 12 0.7228622382194771 1103 13 0.699117306978664 1103 14 0.853277396222083 1103 15 0.6747335074320233 1103 16 0.6990633709272629 1103 17 0.8375933429443521 1103 18 0.9103712138983653 1103 19 0.8517664932489595 1103 20 0.8942595618121345 1103 21 0.9399054893045786 1103 22 1.0521569748511865 1103 23 0.9139989746587389 1103 24 0.9422092285641099 1103 25 0.801401321418254 1104 1 1.362722209324744 1104 2 4.088359550761961 1104 3 2.4444843917926278 1104 4 1.502398270235311 1104 5 1.0395196871837697 1104 6 0.8501658909763776 1104 7 0.6732562835918117 1104 8 0.6748166459059419 1104 9 0.7940427070492717 1104 10 0.6050959834794511 1104 11 0.8481546250962992 1104 12 0.7735103184346883 1104 13 0.7358411114207328 1104 14 0.9622460260271026 1104 15 0.8777193452674581 1104 16 0.8389241948673245 1104 17 0.8509795898595769 1104 18 0.6297191318156194 1104 19 1.0400743702582318 1104 20 0.9279530632239101 1104 21 1.0211311724011338 1104 22 1.0682567102832792 1104 23 0.9390473305581537 1104 24 0.8600242088882624 1104 25 1.0699622053863322 1105 1 1.2081211166804984 1105 2 3.773876251621786 1105 3 2.3058880016679204 1105 4 1.3290355077355969 1105 5 0.8725203095760605 1105 6 0.7899436738767278 1105 7 0.6718639689840344 1105 8 0.4109949267201216 1105 9 0.5616183738158327 1105 10 0.563363427752293 1105 11 0.6453215875913969 1105 12 0.7501844384411456 1105 13 0.7858353485291474 1105 14 0.664611116735017 1105 15 0.508550815265842 1105 16 0.716331354683831 1105 17 0.7510994580046173 1105 18 0.8089346870125951 1105 19 0.6827727866156224 1105 20 0.6562788279596177 1105 21 0.6383933378652742 1105 22 0.7469213330443907 1105 23 0.7591678902501897 1105 24 0.6946384409253241 1105 25 0.6485242486771696 1106 1 0.8313826761886745 1106 2 3.5369951244785547 1106 3 1.907123535092616 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0.9305720796696519 1107 16 0.8253554345712573 1107 17 0.8602582274149739 1107 18 0.7470176770254824 1107 19 1.023835014356699 1107 20 0.9782210508802365 1107 21 0.8071250469689915 1107 22 0.9911748754379204 1107 23 1.0859034061051531 1107 24 1.0411792240858304 1107 25 1.0703659270504033 1108 1 1.5202267823487148 1108 2 4.1867084484225 1108 3 2.565606948835086 1108 4 1.6016280767241895 1108 5 1.1247030895136092 1108 6 1.0180680764004677 1108 7 0.843873401142595 1108 8 0.6876981323794169 1108 9 0.727796437674094 1108 10 0.8098423009917255 1108 11 0.8924696342423459 1108 12 0.7526380742833467 1108 13 0.7590046812676619 1108 14 1.012342300215991 1108 15 0.9553355963505439 1108 16 1.0029128345130331 1108 17 0.9458289207539607 1108 18 1.009097200555388 1108 19 0.9417110314693602 1108 20 0.9067727416681137 1108 21 0.798150523513101 1108 22 0.9289046355745043 1108 23 1.042546897324307 1108 24 0.9956071491019856 1108 25 1.0907044793826728 1109 1 0.9399457094205843 1109 2 3.086045002170817 1109 3 1.969254374541336 1109 4 0.9453156557058833 1109 5 0.6013703676514766 1109 6 0.2214281106428544 1109 7 0.37875273749251903 1109 8 0.3154242748114413 1109 9 0.3386612617742131 1109 10 0.3768901180395474 1109 11 0.40955006308747954 1109 12 0.4316615547774205 1109 13 0.24534849114088075 1109 14 0.41307504653697436 1109 15 0.4826072118574558 1109 16 0.4029169109876989 1109 17 0.29660447005892365 1109 18 0.24714293860112946 1109 19 0.3273659171242471 1109 20 0.3367847672683766 1109 21 0.26948271543352575 1109 22 0.29746890119759684 1109 23 0.33885564951540914 1109 24 0.4354842433908459 1109 25 0.5768291787426634 1110 1 0.8144567678230956 1110 2 3.5703702627662857 1110 3 2.152240767400709 1110 4 1.0809865459957138 1110 5 0.5227834088682073 1110 6 0.42438828791019034 1110 7 0.1927442694401762 1110 8 0.10352530887090694 1110 9 0.21334396988648052 1110 10 0.21524290500664733 1110 11 0.21434793255048135 1110 12 0.07725951994812874 1110 13 0.24118728605589013 1110 14 0.4334415446748352 1110 15 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-1.4982375284986844 2315 11 -1.1645416608782995 2315 12 -1.305786535630368 2315 13 -1.350755169194521 2315 14 -1.6647865051910742 2315 15 -1.6893582044404596 2315 16 -1.898323246045912 2315 17 -1.7380444735950533 2315 18 -1.9211973160111668 2315 19 -1.888748181861395 2315 20 -1.990129363487064 2315 21 -1.829956774434173 2315 22 -1.8102817966034763 2315 23 -1.863308101137001 2315 24 -1.6822824527763558 2315 25 -1.7251286354947482 2316 1 -0.5809917830316529 2316 2 1.9372786084312317 2316 3 0.363003015944134 2316 4 -0.5052253678914411 2316 5 -0.8453756410918558 2316 6 -0.8286316676340846 2316 7 -1.4202865833296414 2316 8 -1.284846236987221 2316 9 -1.6970758828771437 2316 10 -1.871225960505491 2316 11 -1.6467743578482015 2316 12 -1.7152752613953268 2316 13 -1.6815167118637362 2316 14 -1.7984464615663 2316 15 -1.8643603991852231 2316 16 -2.0038229387816386 2316 17 -1.8676559700058064 2316 18 -1.9094942616017576 2316 19 -1.909392288802863 2316 20 -1.9489221863340163 2316 21 -2.074816006881278 2316 22 -1.8597479671452746 2316 23 -1.9316018052583956 2316 24 -1.978353521988474 2316 25 -1.767092155460169 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20776.0 3 0.0 4 158.0 5 867.0 6 1013.0 7 888.0 8 819.0 9 814.0 10 737.0 11 745.0 12 711.0 13 715.0 14 717.0 15 676.0 16 753.0 17 847.0 18 946.0 19 1157.0 20 1420.0 21 1747.0 22 2173.0 23 2588.0 24 2949.0 25 3434.0 26 3984.0 27 4566.0 28 5461.0 29 6725.0 30 8821.0 31 11932.0 32 16461.0 33 23754.0 34 36017.0 35 57361.0 36 102413.0 37 140929.0 38 387894.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.499798587300695 19.78657279898076 13.196864519513612 25.516764094204937 2 15.87768271062513 21.547331084511885 40.949594832849435 21.625391372013553 3 18.316142993648473 25.15586064114815 30.369404942573965 26.158591422629417 4 11.540713469357165 17.637897438779905 39.882993273752646 30.93839581811028 5 12.084996159106664 38.30986641185618 36.904778633391416 12.700358795645739 6 29.35953103629176 39.01164914844584 19.145682273808852 12.483137541453544 7 25.91549097858468 32.380721525865134 23.889068442845634 17.81471905270455 8 23.24770951604744 38.070278980008624 21.78500833754895 16.897003166394995 9 25.25820639649261 15.74988758360969 21.53581867236243 37.456087347535274 10 13.844429767860152 29.582256009592868 35.79654038558822 20.77677383695876 11 32.34559140389335 23.180845183894476 26.18447061248197 18.2890927997302 12 22.080218462518502 25.149537219193224 33.476898431791355 19.29334588649692 13 27.74401382721601 22.82181533734285 26.823955932775 22.610214902666144 14 19.49253367807693 22.994187135817736 29.388571937121768 28.124707248983565 15 21.48558259794278 31.831169317819874 24.8516337848725 21.831614299364848 16 20.45650422498267 28.942419388080115 29.421360050962097 21.17971633597512 17 20.20192794109381 29.06256440522362 29.455553369681297 21.279954284001274 18 19.847699211211662 27.908891199670244 31.88222509508553 20.361184494032564 19 21.721967527943185 27.305100326125782 29.616553371625297 21.35637877430574 20 21.704560358233564 27.958424671650462 30.24375620632155 20.093258763794427 21 21.17795982987653 27.38638918554325 29.268778221197984 22.166872763382234 22 21.19799802800606 28.919689258722325 29.5375182386019 20.344794474669715 23 20.344746826549255 28.504496697737597 29.882078785891608 21.268677689821537 24 21.183580649392013 27.401495137991116 30.705951511063645 20.70897270155322 25 20.829469019916438 28.082785303430573 30.235910479081184 20.851835197571805 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 246.0 1 246.0 2 351.5 3 457.0 4 457.0 5 457.0 6 1117.0 7 1777.0 8 1777.0 9 1777.0 10 2441.5 11 3106.0 12 3106.0 13 3106.0 14 4680.0 15 6254.0 16 6254.0 17 6254.0 18 10335.0 19 14416.0 20 14416.0 21 14416.0 22 21840.0 23 29264.0 24 29264.0 25 29264.0 26 40758.5 27 52253.0 28 52253.0 29 52253.0 30 65227.5 31 78202.0 32 78202.0 33 78202.0 34 86086.5 35 93971.0 36 93971.0 37 93971.0 38 98351.0 39 102731.0 40 102731.0 41 102731.0 42 104977.0 43 107223.0 44 107223.0 45 107223.0 46 106931.5 47 106640.0 48 106640.0 49 106640.0 50 99697.5 51 92755.0 52 92755.0 53 92755.0 54 80798.5 55 68842.0 56 68842.0 57 68842.0 58 57508.0 59 46174.0 60 46174.0 61 46174.0 62 36680.5 63 27187.0 64 27187.0 65 27187.0 66 20413.5 67 13640.0 68 13640.0 69 13640.0 70 9722.0 71 5804.0 72 5804.0 73 5804.0 74 3913.0 75 2022.0 76 2022.0 77 2022.0 78 1363.0 79 704.0 80 704.0 81 704.0 82 457.0 83 210.0 84 210.0 85 210.0 86 135.0 87 60.0 88 60.0 89 60.0 90 38.5 91 17.0 92 17.0 93 17.0 94 12.5 95 8.0 96 8.0 97 8.0 98 6.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.291847001292789 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 3.5130121971783484E-4 20 0.0 21 0.0 22 0.0011710040657261162 23 9.368032525808929E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 853968.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.0123353989565 #Duplication Level Percentage of deduplicated Percentage of total 1 87.25755243526723 68.071654466721 2 9.538586317007745 14.882547899886106 3 1.887546934878174 4.417558336949652 4 0.5763571837510576 1.7985187971354197 5 0.23439404991500287 0.9142813618744475 6 0.12034668213688265 0.5633115438608456 7 0.07582775578427317 0.4140850221755017 8 0.0514494773288345 0.32109551051824364 9 0.03656124074165094 0.25670049978056986 >10 0.18664054810877034 2.661852063868173 >50 0.017900818345449692 1.013487196479975 >100 0.014409665812581147 2.205664622965741 >500 0.0012134454612350537 0.6445415648096455 >1k 0.0012134454612350537 1.8347011129747668 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3857 0.45165626815056303 No Hit TATCAACGCAGAGTACTTTTTTTTT 2336 0.27354654975362075 No Hit GGTATCAACGCAGAGTACTTTTTTT 2202 0.25785509527289074 No Hit GTCCTACAGTGGACATTTCTAAATT 1669 0.1954405785696888 No Hit CTGTAGGACGTGGAATATGGCAAGA 1627 0.1905223614936391 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1445 0.1692100874974238 No Hit CTTTAGGACGTGAAATATGGCGAGG 1349 0.15796844846645308 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1020 0.11944241470406385 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 985 0.11534390047402245 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.1710040657261162E-4 0.0 0.0 0.0 6 1.1710040657261162E-4 1.1710040657261162E-4 0.0 0.0 0.0 7 1.1710040657261162E-4 2.3420081314522325E-4 0.0 0.0 0.0 8 1.1710040657261162E-4 2.3420081314522325E-4 0.0 0.0 0.0 9 1.1710040657261162E-4 2.3420081314522325E-4 0.0 0.0 0.0 10 1.1710040657261162E-4 2.3420081314522325E-4 0.0 0.0 0.0 11 1.1710040657261162E-4 2.3420081314522325E-4 0.0 1.1710040657261162E-4 0.0 12 1.1710040657261162E-4 2.3420081314522325E-4 0.0 1.1710040657261162E-4 0.0 13 1.1710040657261162E-4 2.3420081314522325E-4 0.0 1.1710040657261162E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTATCC 35 0.0022358145 16.212038 3 GCCCTAA 55 1.4015469E-4 14.373392 1 TAGGACC 205 0.0 14.300863 4 CTAGGAC 125 7.2759576E-12 13.6181135 3 GGTATCA 490 0.0 13.511723 1 ACGAAAT 65 5.683539E-5 13.094339 12 CCGGTTT 60 4.2480542E-4 12.6093645 12 TCCAACG 85 4.1421245E-6 12.238502 18 GCCGGTT 65 8.327669E-4 11.639413 11 GACCATA 65 8.327669E-4 11.639413 7 GTGTTAA 95 8.769071E-6 11.441977 1 CTACTGG 70 0.0010722118 11.293379 1 GTCTAAG 105 2.15605E-6 11.293379 1 ACTGTTC 210 0.0 11.258361 8 TATACTG 135 5.0586095E-9 11.2083235 5 GATATAC 235 0.0 10.932953 1 TAGGACT 130 3.481E-8 10.91195 4 GTTTAAG 100 1.5492313E-5 10.869877 1 GTCGAGG 70 0.0015476445 10.808025 19 CGGTTTC 70 0.0015476445 10.808025 13 >>END_MODULE