FastQCFastQC Report
Thu 26 May 2016
SRR1511678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511678_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853968
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54320.6360894085024263No Hit
TATCAACGCAGAGTACTTTTTTTTT38230.4476748543270942No Hit
GGTATCAACGCAGAGTACTTTTTTT35600.41687744739849736No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17640.2065651171940869No Hit
CTGTAGGACGTGGAATATGGCAAGA15070.1764703127049257No Hit
CTTTAGGACGTGAAATATGGCGAGG13620.15949075375189703No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13560.15878815131246135No Hit
GTCCTACAGTGGACATTTCTAAATT12980.15199632773124988No Hit
GTCCTAAAGTGTGTATTTCTCATTT12650.1481320143143537No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11610.1359535720308021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8050.013.5012761
CTATACT852.6931957E-713.4121794
CTCGAAC701.0924593E-412.21108618
TCTATAC907.4468826E-611.6114693
TAAGACC1001.9253239E-611.40035254
TAGGACC1751.8189894E-1211.4003524
AGACTGT1251.8335413E-811.3970146
TCGAACT855.330323E-511.17354319
AGACCTA600.005883448811.080436
GATAGCC600.005883448811.080437
CCCTATA700.001489826810.8574772
TAGACCT700.001489826810.8574775
GTGGTAT2400.010.725521
CCTACAC803.7658503E-410.687833
GAACCAT803.776055E-410.6847016
GGCGAGG4300.010.381000519
TGTAGGA8600.010.2735722
TGGTATC2600.010.2310852
TTAGGAC5450.010.1104033
AATGTCC2450.010.0789918
Produced by FastQC (version 0.11.5)