FastQCFastQC Report
Thu 26 May 2016
SRR1511677_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511677_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249386
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48180.38562942117167953No Hit
TATCAACGCAGAGTACTTTTTTTTT30130.24115845703409516No Hit
GGTATCAACGCAGAGTACTTTTTTT30000.2401179459350433No Hit
GTCCTACAGTGGACATTTCTAAATT25720.20586111898164378No Hit
CTGTAGGACGTGGAATATGGCAAGA23510.18817243029776226No Hit
GTCCTAAAGTGTGTATTTCTCATTT23040.18441058247811323No Hit
CTTTAGGACGTGAAATATGGCGAGG20040.16039878788460893No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13350.10685248594109427No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13090.10477146374299057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG454.9905304E-415.3808762
GGTATCA8550.014.2244191
GTCGAGG801.362223E-714.18456619
CGTGCAT400.005441959514.18456612
TCCAACG1550.014.03204318
GCCTAAG653.743204E-513.690671
TATGTCG852.853012E-713.35018116
GGCGAGG5950.013.3501819
TTAGGAC8550.012.8297053
CGTAGGA801.901557E-512.35963252
CCAACGT1104.062713E-812.0353919
AGGACGT14100.012.0049055
ATGTCGA951.0986059E-611.94489817
ATAAGAC951.0986059E-611.9448983
GACGTGA7550.011.8987537
GGACGTG14100.011.8707726
CGCCACC803.0034536E-511.82047214
ACGTGAA8100.011.674548
CGTGAAA8200.011.53216659
AATCCCG1407.3305273E-1011.48274419
Produced by FastQC (version 0.11.5)