FastQCFastQC Report
Thu 26 May 2016
SRR1511677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511677_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249386
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62440.49976548480613675No Hit
TATCAACGCAGAGTACTTTTTTTTT43800.35057220106516324No Hit
GGTATCAACGCAGAGTACTTTTTTT41140.329281743192256No Hit
CTGTAGGACGTGGAATATGGCAAGA23860.1909738063336711No Hit
GTCCTACAGTGGACATTTCTAAATT22580.18072877397377593No Hit
GTCCTAAAGTGTGTATTTCTCATTT20280.16231973145208928No Hit
CTTTAGGACGTGAAATATGGCGAGG19710.15775749047932344No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19650.15727725458745337No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16120.12902337628242994No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14090.11277539527415865No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG400.005149807314.30304051
CCGTGCT551.964576E-413.8141079
AAGGCGT1051.375156E-913.5674276
CGAAACA500.001502596113.29607913
GGTATCA9750.012.4204171
GGCGTGC752.0788811E-411.3966388
AATCCCG1701.0913936E-1111.17317519
ACCGGGG600.005887476711.08006515
ACGCTGT600.005887476711.0800656
TAGGACC3650.010.4120564
CCACCTT3200.010.38756113
AAAGGCG1106.0333878E-610.3647285
GTATCAA19500.010.3177481
CAAGTCG1303.8528378E-710.2277526
TTCCACC3300.010.07278611
GATATAC4450.010.0710541
GTACTAG951.582758E-410.037221
TATACTG1901.0004442E-1010.0010535
GGCGAGG5800.09.98843319
ATTTCTA9650.09.94007315
Produced by FastQC (version 0.11.5)