##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511677_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1249386 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.628216579984088 31.0 30.0 34.0 26.0 34.0 2 29.824612249536973 31.0 30.0 34.0 26.0 34.0 3 29.88048849594921 31.0 30.0 34.0 26.0 34.0 4 33.480048599872255 35.0 35.0 37.0 28.0 37.0 5 32.99913157342887 35.0 33.0 37.0 26.0 37.0 6 32.94619116910226 35.0 33.0 37.0 26.0 37.0 7 32.70090108261178 35.0 33.0 37.0 25.0 37.0 8 32.56671116852598 35.0 33.0 37.0 25.0 37.0 9 33.85306782691658 37.0 33.0 39.0 25.0 39.0 10 33.55692316065652 37.0 33.0 39.0 23.0 39.0 11 33.57778941015827 37.0 33.0 39.0 23.0 39.0 12 33.327032638431994 37.0 33.0 39.0 22.0 39.0 13 33.30492177757714 37.0 33.0 39.0 22.0 39.0 14 34.023880530116394 38.0 33.0 40.0 19.0 41.0 15 33.91737541480375 38.0 33.0 40.0 19.0 41.0 16 33.71539940418734 38.0 33.0 40.0 17.0 41.0 17 33.65208110223742 38.0 33.0 40.0 17.0 41.0 18 33.54348375922253 38.0 33.0 40.0 17.0 41.0 19 33.56555380002657 38.0 33.0 40.0 18.0 41.0 20 33.67016678592525 38.0 33.0 40.0 18.0 41.0 21 33.58365949354323 38.0 33.0 40.0 16.0 41.0 22 33.42798622683462 38.0 33.0 40.0 11.0 41.0 23 33.2642193845617 38.0 33.0 40.0 10.0 41.0 24 33.02845797855907 38.0 32.0 40.0 2.0 41.0 25 32.40220476297957 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2938143560493707 1101 2 0.41898766641160634 1101 3 0.5862334307625652 1101 4 0.5204215701323847 1101 5 0.693296901515069 1101 6 0.6900554461072375 1101 7 0.6739736475353837 1101 8 0.6458343552799448 1101 9 0.5431442872995191 1101 10 0.6808166367265684 1101 11 0.5831524437283804 1101 12 0.6394950488549895 1101 13 0.7480467116263867 1101 14 0.5683713953661851 1101 15 0.7466752952694833 1101 16 0.5517260900531014 1101 17 0.3142588298860005 1101 18 0.5854806790432079 1101 19 0.5329733050988708 1101 20 0.3289740718711869 1101 21 0.2960748552396666 1101 22 0.2998861312881118 1101 23 0.5917306709165189 1101 24 0.30095425410548415 1101 25 0.32355633637332915 1102 1 0.8358751546629897 1102 2 0.8961553061586756 1102 3 0.8808343818369089 1102 4 1.0796825533905903 1102 5 1.1093667827855072 1102 6 1.159505277451423 1102 7 0.9755161776819676 1102 8 1.0364317939810377 1102 9 1.1249571825059803 1102 10 1.172420594722503 1102 11 1.0841091915080057 1102 12 1.3188904152042866 1102 13 1.0786353049234805 1102 14 1.3344775092681687 1102 15 1.2646046455062177 1102 16 1.0740394597651957 1102 17 1.303695921785362 1102 18 1.0282872767346376 1102 19 1.2851098698843586 1102 20 1.143477826654923 1102 21 0.8664467490329244 1102 22 1.1198905410959341 1102 23 1.3372099731869227 1102 24 1.1702750147586514 1102 25 1.4075754249098864 1103 1 0.3452601478266537 1103 2 0.3612950137603903 1103 3 0.3820646713451872 1103 4 0.5252692776734662 1103 5 0.4032012927039901 1103 6 0.575620847556614 1103 7 0.38657768714218577 1103 8 0.5283996491021483 1103 9 0.511093810152353 1103 10 0.7259079404504689 1103 11 0.46983896163037286 1103 12 0.44514726956245454 1103 13 0.5136167769202089 1103 14 0.6227859035099783 1103 15 0.4639297259217017 1103 16 0.447067626969222 1103 17 0.7332873152128272 1103 18 0.2890754268368525 1103 19 0.4669350615744392 1103 20 0.39653254930535553 1103 21 0.5662504669208062 1103 22 0.5087052817509061 1103 23 0.6216510268037254 1103 24 0.49980892153749323 1103 25 0.46727001220025954 1104 1 0.33287741396553017 1104 2 0.624420048248151 1104 3 0.5710173036061406 1104 4 0.7271508679359187 1104 5 0.5710750051867279 1104 6 0.706741921454352 1104 7 0.685591693668357 1104 8 0.827331815007966 1104 9 0.9438192702113426 1104 10 1.2022476311053225 1104 11 1.1051298771355107 1104 12 1.2397018922864973 1104 13 0.8570820203867555 1104 14 1.1257200757509978 1104 15 1.23825235528723 1104 16 1.2828742623835154 1104 17 1.205118951696626 1104 18 1.2380143197936633 1104 19 1.1730925142479265 1104 20 1.0797194478620469 1104 21 1.0621575058961596 1104 22 1.145302547158181 1104 23 1.2970897266290677 1104 24 1.5130943822412917 1104 25 1.504871437219066 1105 1 0.19838804323175196 1105 2 0.11933351621397748 1105 3 0.20106968146838256 1105 4 0.3754116887094625 1105 5 0.3263079122434718 1105 6 0.38944844441327575 1105 7 0.41822124776124525 1105 8 0.35789674940755845 1105 9 0.46024620711882847 1105 10 0.37989201786716364 1105 11 0.39921681414057986 1105 12 0.4227389788380478 1105 13 0.43078245414474026 1105 14 0.4106921181216876 1105 15 0.3265030761842169 1105 16 0.5390214034353704 1105 17 0.6965993154873544 1105 18 0.6287471951358157 1105 19 0.5653702559740807 1105 20 0.7016095885284415 1105 21 0.9074878987179318 1105 22 0.7434561482276933 1105 23 0.9158413428532768 1105 24 0.6730956996164892 1105 25 0.9634137615568363 1106 1 0.344488744527613 1106 2 0.5112105629618284 1106 3 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16 1.0042924986054231 1107 17 1.0358510092246576 1107 18 0.9665651657615015 1107 19 1.0427465912806042 1107 20 0.9704326719565444 1107 21 0.8868277056756142 1107 22 1.0296066259733934 1107 23 0.9968685690121646 1107 24 1.232468846711626 1107 25 1.0725820563497521 1108 1 0.41999952057809153 1108 2 0.49593870616939384 1108 3 0.3199299489682872 1108 4 0.4109830791754874 1108 5 0.6078306040088961 1108 6 0.3976982634126287 1108 7 0.4255422476469306 1108 8 0.7800617076821439 1108 9 0.7992842363075638 1108 10 0.5104268271496437 1108 11 0.6986014119380854 1108 12 0.7043902763269045 1108 13 0.575421024430149 1108 14 0.8802887724676083 1108 15 0.9178369556949448 1108 16 0.9598907781257608 1108 17 0.8969813221176892 1108 18 1.1227884632835128 1108 19 0.9267666283400473 1108 20 1.1450992810178704 1108 21 1.2208667044947106 1108 22 1.122504846928301 1108 23 1.0895495698090372 1108 24 0.9813731450305099 1108 25 1.0641596747527373 1109 1 -0.11876222306477757 1109 2 0.018795149788029875 1109 3 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2313 24 -0.7095777752148891 2313 25 -0.5501041289282433 2314 1 -0.59442713219212 2314 2 -0.6331929232689824 2314 3 -0.5930926485635126 2314 4 -0.714902227691411 2314 5 -0.8776622069440378 2314 6 -0.7822067511549662 2314 7 -0.8834280723663319 2314 8 -0.6917884948429034 2314 9 -0.9607123290571025 2314 10 -1.7872642688543365 2314 11 -1.1859770754011336 2314 12 -1.046859812499875 2314 13 -1.027892214312537 2314 14 -1.195414143419356 2314 15 -1.1767452031510146 2314 16 -0.863165099838092 2314 17 -1.0497285341013693 2314 18 -0.7382118446493138 2314 19 -0.5942906471650815 2314 20 -0.7744437940466824 2314 21 -0.899629777964968 2314 22 -0.784876065974089 2314 23 -1.0124111332252852 2314 24 -1.1329392172879906 2314 25 -1.0014217261047342 2315 1 -0.9849108932641144 2315 2 -1.0667155214974642 2315 3 -1.0661110451113203 2315 4 -1.3690017053203007 2315 5 -1.5503399266805822 2315 6 -1.4850911481570073 2315 7 -1.596305655832154 2315 8 -1.5942640796714294 2315 9 -1.7335717430929876 2315 10 -1.8413183229083927 2315 11 -1.8788001505771526 2315 12 -1.6901484793179513 2315 13 -1.6054983919187151 2315 14 -1.6684325399671636 2315 15 -1.7980488597487891 2315 16 -1.8132445910940511 2315 17 -1.928447589291011 2315 18 -1.8488409611607821 2315 19 -1.6832300066926784 2315 20 -1.6690838122161118 2315 21 -1.6570678883442511 2315 22 -1.7477195817587798 2315 23 -2.013682993320675 2315 24 -1.5601025150395245 2315 25 -1.5758936770472758 2316 1 -1.3875943253593128 2316 2 -1.3987480888241492 2316 3 -1.518657174128041 2316 4 -1.3895249023140863 2316 5 -1.7650144942963273 2316 6 -2.063498432446643 2316 7 -2.205786794725057 2316 8 -2.901967505021915 2316 9 -2.3624439307887 2316 10 -2.3106057921958616 2316 11 -2.1721710615951224 2316 12 -2.3445783102183704 2316 13 -2.6595524459727713 2316 14 -2.7581613061665173 2316 15 -2.6027894291952443 2316 16 -2.428603265276255 2316 17 -2.5590300434028777 2316 18 -2.386322292759761 2316 19 -2.533497386371824 2316 20 -2.630229449832335 2316 21 -2.3916152699504565 2316 22 -2.5993840804821033 2316 23 -2.926579847393999 2316 24 -2.851811336160111 2316 25 -2.6544695791525896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39493.0 3 1.0 4 660.0 5 5289.0 6 6637.0 7 5296.0 8 4302.0 9 3908.0 10 3623.0 11 3596.0 12 3307.0 13 3267.0 14 3031.0 15 3067.0 16 3107.0 17 3188.0 18 3387.0 19 3890.0 20 4830.0 21 5974.0 22 7601.0 23 9498.0 24 11486.0 25 12949.0 26 14938.0 27 17230.0 28 19838.0 29 24163.0 30 30148.0 31 38395.0 32 48301.0 33 61996.0 34 81257.0 35 109071.0 36 158306.0 37 242418.0 38 251938.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.419208107111324 21.826510581846904 14.655088599214006 23.099192711827772 2 15.686505211359819 23.026830779278782 40.04158842823595 21.245075581125448 3 19.25978040413451 27.01663056893546 31.027800855780356 22.69578817114967 4 13.591556172391877 19.63492467500036 38.45488904149718 28.318630111110576 5 12.597859070128663 39.04466809181819 36.22142691294566 12.136045925107487 6 29.897965880840673 39.64923570457809 18.26945395578308 12.183344458798162 7 26.563127808379477 32.79514897717759 23.3558724045251 17.285850809917832 8 24.2308621995124 38.5118770339991 21.05282114574679 16.20443962074171 9 26.27226493653683 15.521704261133069 20.897304756096194 37.30872604623391 10 14.705863520161103 30.247417531491465 35.05369837664261 19.99302057170482 11 33.682304748092264 23.03667561506212 25.408960881585035 17.872058755260582 12 22.764702021633028 25.840933066322176 32.61377988868132 18.78058502336348 13 28.90867994358829 22.867552541808536 26.375515653288893 21.848251861314278 14 20.501190184618682 23.27575304989811 28.644310085113805 27.5787466803694 15 22.463353999484546 32.247680060445695 24.074865573969934 21.214100366099828 16 21.37225805315571 29.36226274345959 28.494716604796277 20.770762598588426 17 20.930120875373984 29.48336222752616 28.717305940678063 20.869210956421792 18 20.77324381736309 28.14110290974927 31.11128186165044 19.9743714112372 19 22.81056454930662 27.46885270044646 29.026497815727087 20.694084934519836 20 22.73396692455334 28.044175298906822 29.551075488279842 19.670782288259993 21 22.349217935850092 27.338468655803734 28.690572809363964 21.62174059898222 22 22.375710949218256 28.719867198768036 28.89443294546281 20.0099889065509 23 21.563712095381252 28.473586225553994 29.2047453709262 20.75795630813856 24 22.243165843062112 27.614444214998407 29.808481926322212 20.333908015617272 25 22.02449416060556 28.09416668867795 29.379705913192282 20.501633237524203 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 347.0 1 347.0 2 508.5 3 670.0 4 670.0 5 670.0 6 1637.5 7 2605.0 8 2605.0 9 2605.0 10 3591.5 11 4578.0 12 4578.0 13 4578.0 14 6635.0 15 8692.0 16 8692.0 17 8692.0 18 14678.5 19 20665.0 20 20665.0 21 20665.0 22 31752.5 23 42840.0 24 42840.0 25 42840.0 26 59886.0 27 76932.0 28 76932.0 29 76932.0 30 95738.5 31 114545.0 32 114545.0 33 114545.0 34 125436.0 35 136327.0 36 136327.0 37 136327.0 38 141750.0 39 147173.0 40 147173.0 41 147173.0 42 151295.5 43 155418.0 44 155418.0 45 155418.0 46 154670.5 47 153923.0 48 153923.0 49 153923.0 50 145346.5 51 136770.0 52 136770.0 53 136770.0 54 118717.5 55 100665.0 56 100665.0 57 100665.0 58 84313.5 59 67962.0 60 67962.0 61 67962.0 62 54859.5 63 41757.0 64 41757.0 65 41757.0 66 31865.5 67 21974.0 68 21974.0 69 21974.0 70 15923.0 71 9872.0 72 9872.0 73 9872.0 74 6890.0 75 3908.0 76 3908.0 77 3908.0 78 2599.5 79 1291.0 80 1291.0 81 1291.0 82 825.0 83 359.0 84 359.0 85 359.0 86 224.5 87 90.0 88 90.0 89 90.0 90 54.5 91 19.0 92 19.0 93 19.0 94 10.5 95 2.0 96 2.0 97 2.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38811063994634165 2 0.0 3 0.0 4 0.0 5 0.03169556886342571 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0021610615134153893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1249386.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.19772390045316 #Duplication Level Percentage of deduplicated Percentage of total 1 88.1122092528717 68.02062002162143 2 8.753958762692763 13.51571383196617 3 1.7506708190073952 4.054434075789392 4 0.5390850556330116 1.6646455713447064 5 0.2584207972087187 0.997474867652683 6 0.1428514109482263 0.6616682268702808 7 0.09196073722566649 0.49694117214203887 8 0.058389700449534516 0.3606041579146665 9 0.045823243158843986 0.31837050662397903 >10 0.2083424769547407 2.903913811184014 >50 0.019068290904127417 1.0166973818950789 >100 0.015809580169558836 2.4334170803272057 >500 0.00216991903911124 1.1090145759489922 >1k 0.0011366242585820782 1.9484132944062496 >5k 1.0332947805291618E-4 0.49807142431313445 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6244 0.49976548480613675 No Hit TATCAACGCAGAGTACTTTTTTTTT 4380 0.35057220106516324 No Hit GGTATCAACGCAGAGTACTTTTTTT 4114 0.329281743192256 No Hit CTGTAGGACGTGGAATATGGCAAGA 2386 0.1909738063336711 No Hit GTCCTACAGTGGACATTTCTAAATT 2258 0.18072877397377593 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2028 0.16231973145208928 No Hit CTTTAGGACGTGAAATATGGCGAGG 1971 0.15775749047932344 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1965 0.15727725458745337 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1612 0.12902337628242994 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1409 0.11277539527415865 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.401179459350433E-4 0.0 0.0 0.0 0.0 7 2.401179459350433E-4 0.0 0.0 0.0 0.0 8 2.401179459350433E-4 0.0 0.0 0.0 0.0 9 2.401179459350433E-4 0.0 0.0 0.0 0.0 10 2.401179459350433E-4 0.0 0.0 0.0 0.0 11 2.401179459350433E-4 0.0 0.0 0.0 0.0 12 2.401179459350433E-4 0.0 0.0 0.0 0.0 13 2.401179459350433E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTAAG 40 0.0051498073 14.3030405 1 CCGTGCT 55 1.964576E-4 13.814107 9 AAGGCGT 105 1.375156E-9 13.567427 6 CGAAACA 50 0.0015025961 13.296079 13 GGTATCA 975 0.0 12.420417 1 GGCGTGC 75 2.0788811E-4 11.396638 8 AATCCCG 170 1.0913936E-11 11.173175 19 ACCGGGG 60 0.0058874767 11.080065 15 ACGCTGT 60 0.0058874767 11.080065 6 TAGGACC 365 0.0 10.412056 4 CCACCTT 320 0.0 10.387561 13 AAAGGCG 110 6.0333878E-6 10.364728 5 GTATCAA 1950 0.0 10.317748 1 CAAGTCG 130 3.8528378E-7 10.227752 6 TTCCACC 330 0.0 10.072786 11 GATATAC 445 0.0 10.071054 1 GTACTAG 95 1.582758E-4 10.03722 1 TATACTG 190 1.0004442E-10 10.001053 5 GGCGAGG 580 0.0 9.988433 19 ATTTCTA 965 0.0 9.940073 15 >>END_MODULE