FastQCFastQC Report
Thu 26 May 2016
SRR1511676_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511676_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746991
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43710.5851476122202276No Hit
TATCAACGCAGAGTACTTTTTTTTT25810.34551955779922383No Hit
GGTATCAACGCAGAGTACTTTTTTT24730.331061552281085No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11170.1495332607755649No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10880.1456510185531017No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9130.12222369479685832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG350.002234824716.212735
TATGGAC400.005435249314.186143
TAGGGTG400.005435249314.186145
GCCTCGA707.5946937E-613.51060916
CTAAGGC500.001549844313.2403973
GGTATCA4800.013.1706171
GTCTTAG450.00888574113.1706171
ACGTGTC604.245418E-412.6099038
GGCGAGG854.13819E-612.23902319
TAGGACC701.13607726E-412.1595484
TCGAACT701.13607726E-412.15954819
GGCCTCG907.827597E-611.55907615
TGGCGAG1407.2941475E-1011.48401718
GTATAGC958.801808E-611.4376411
GACGTGG752.1592698E-411.3489127
GTTTAGG802.6241757E-411.1127081
GGACGTG1451.3042154E-911.0880176
GTGAGCG600.006070360511.0336669
ATACTAT951.4267354E-510.9507056
GACGTGA700.001546700810.8084877
Produced by FastQC (version 0.11.5)