##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511676_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 746991 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.180186909882448 31.0 30.0 34.0 25.0 34.0 2 27.553698772809845 31.0 28.0 34.0 10.0 34.0 3 29.20810692498303 31.0 30.0 34.0 16.0 34.0 4 33.70256669759073 37.0 35.0 37.0 28.0 37.0 5 34.145066004811305 37.0 35.0 37.0 31.0 37.0 6 34.39561520821536 37.0 35.0 37.0 32.0 37.0 7 34.467326915585325 37.0 35.0 37.0 32.0 37.0 8 34.50543580846355 37.0 35.0 37.0 32.0 37.0 9 36.19691937386126 39.0 37.0 39.0 32.0 39.0 10 36.11790369629621 39.0 37.0 39.0 32.0 39.0 11 36.131337593090144 39.0 37.0 39.0 32.0 39.0 12 36.06197397291266 39.0 37.0 39.0 32.0 39.0 13 36.04775559544894 39.0 37.0 39.0 32.0 39.0 14 37.34498006000072 40.0 38.0 41.0 32.0 41.0 15 37.28399405079847 40.0 38.0 41.0 32.0 41.0 16 37.23660793771277 40.0 38.0 41.0 32.0 41.0 17 37.221338677440556 40.0 38.0 41.0 32.0 41.0 18 37.1843703605532 40.0 38.0 41.0 32.0 41.0 19 37.223738974097415 40.0 38.0 41.0 32.0 41.0 20 37.16934206703963 40.0 38.0 41.0 32.0 41.0 21 37.10600930934911 40.0 38.0 41.0 32.0 41.0 22 37.02111270416913 40.0 38.0 41.0 32.0 41.0 23 36.944629854978174 40.0 38.0 41.0 32.0 41.0 24 36.85815759493756 40.0 38.0 41.0 31.0 41.0 25 36.767698673745734 40.0 38.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9324696459992197 1101 2 3.659054206023569 1101 3 2.0978218423785613 1101 4 1.0815852239982107 1101 5 0.7126942454666008 1101 6 0.34061070313887143 1101 7 0.37597515514942614 1101 8 0.1104750051973511 1101 9 0.2811117990962373 1101 10 0.3415584076719824 1101 11 0.2443554907007197 1101 12 0.20688522214125982 1101 13 0.3195069130989694 1101 14 0.484574918687116 1101 15 0.3283399693804583 1101 16 0.5645887087192918 1101 17 0.5121955917051721 1101 18 0.40994479102414516 1101 19 0.435434129297839 1101 20 0.5039152717136943 1101 21 0.5456956339993368 1101 22 0.2790594708207834 1101 23 0.5051867111444324 1101 24 0.483235014560222 1101 25 0.4145288521550441 1102 1 1.636717225306981 1102 2 4.287908144217905 1102 3 2.8444025588678024 1102 4 1.7298448220652247 1102 5 1.300253759723276 1102 6 1.0324516774786403 1102 7 1.0486066214737235 1102 8 1.0132059740092103 1102 9 0.9750836958846989 1102 10 1.1011252687073068 1102 11 1.1374839345032086 1102 12 1.0148213479637604 1102 13 1.0337181151532135 1102 14 1.0062095769370814 1102 15 1.0927259249074837 1102 16 1.169035114682309 1102 17 1.173414586337742 1102 18 1.177504840240431 1102 19 1.1954200420280898 1102 20 1.1646884666708104 1102 21 1.0327228634435173 1102 22 0.9747566123532607 1102 23 1.061237104339746 1102 24 0.8552905077038275 1102 25 0.9317946735855287 1103 1 1.486050661783608 1103 2 4.187777496048266 1103 3 2.8145081947891697 1103 4 1.6785912013059132 1103 5 1.1998179793153483 1103 6 1.1454623442140672 1103 7 1.0060084419528224 1103 8 0.8866749407712788 1103 9 1.0919407478775156 1103 10 0.8260335141539628 1103 11 1.0040551936249429 1103 12 1.055743448992608 1103 13 0.9895470004320899 1103 14 1.3790682792225653 1103 15 1.2402552133047209 1103 16 1.290249970754438 1103 17 1.1867544520795619 1103 18 1.2314487818613244 1103 19 1.1896397200491151 1103 20 1.1609538199795608 1103 21 1.216549995286428 1103 22 1.2395985022821776 1103 23 1.1366606364817713 1103 24 1.4009486048819966 1103 25 1.2567476144586394 1104 1 1.776852802679585 1104 2 4.424952973057962 1104 3 2.8724270770746436 1104 4 1.8539552578867387 1104 5 1.4206760754471333 1104 6 1.2966457453192675 1104 7 1.1819633302161208 1104 8 1.1984950499013678 1104 9 1.2412387008195083 1104 10 1.2481306190787151 1104 11 1.3454081981778216 1104 12 1.3039361991403169 1104 13 1.2227082974814039 1104 14 1.4915869599301743 1104 15 1.449148967866293 1104 16 1.4036722763881926 1104 17 1.4202459197749704 1104 18 1.2946156396794208 1104 19 1.4388229821351004 1104 20 1.4154806333115033 1104 21 1.4360265879253618 1104 22 1.4851846420660024 1104 23 1.4618526567785821 1104 24 1.585044806181827 1104 25 1.5562297678663484 1105 1 1.4526624396807506 1105 2 4.012029232969624 1105 3 2.4814331615477094 1105 4 1.4854913360328936 1105 5 1.0728899012147863 1105 6 0.7648131304149715 1105 7 0.7754374519883669 1105 8 0.6947491668249555 1105 9 0.697309573991653 1105 10 0.7165759149946069 1105 11 0.6341826705907394 1105 12 0.790660044479992 1105 13 0.8171639766185805 1105 14 0.8500623529965949 1105 15 0.8233618002505878 1105 16 0.7740387047611108 1105 17 0.772561449076818 1105 18 0.8257239125523697 1105 19 0.9516763575689637 1105 20 1.0617533761730371 1105 21 1.1295146052386116 1105 22 0.9578503390012969 1105 23 1.1424547771790046 1105 24 1.0325965301158604 1105 25 1.0840651973568214 1106 1 1.0489036052352994 1106 2 3.557920091649205 1106 3 1.99613493640663 1106 4 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0.6059123453133921 1107 17 0.6645072816630275 1107 18 0.6426601948965072 1107 19 0.7730501891260815 1107 20 0.9536633466257598 1107 21 0.9275063257634528 1107 22 0.8434033564879897 1107 23 0.7062340223590553 1107 24 0.930492358414206 1107 25 0.6653654335791401 1108 1 1.3572758327351941 1108 2 3.8147959836909315 1108 3 2.3569137069991193 1108 4 1.3116847264857725 1108 5 0.9584899150062256 1108 6 0.8446796668988128 1108 7 0.6510371053217412 1108 8 0.574663346461584 1108 9 0.665946588019743 1108 10 0.4336539478965946 1108 11 0.5669948547001837 1108 12 0.5351908769657285 1108 13 0.59967298415215 1108 14 0.6015970423836166 1108 15 0.8627677026730254 1108 16 0.7943951821141582 1108 17 0.7009541038818057 1108 18 0.6595355058240244 1108 19 0.7344878364343685 1108 20 0.6019812797019242 1108 21 0.64580966053758 1108 22 0.6555827443379627 1108 23 0.6888856938238632 1108 24 0.7103548570889373 1108 25 0.8441302051850315 1109 1 0.8579695972234909 1109 2 2.9412217994290906 1109 3 1.7367351191320495 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-1.398879059896828 2315 13 -1.6665970498449312 2315 14 -1.6054062101514504 2315 15 -1.5770254449273153 2315 16 -2.0373155630254303 2315 17 -1.710355446208709 2315 18 -1.8074166774953255 2315 19 -1.7766734363223406 2315 20 -1.8329992933918149 2315 21 -1.8398979498057741 2315 22 -1.8783002821385608 2315 23 -2.00564276217861 2315 24 -1.9985800480577183 2315 25 -1.9065596707476828 2316 1 -0.8301047225142959 2316 2 1.5985112236062804 2316 3 0.3543433889483829 2316 4 -0.5518167571937553 2316 5 -0.9353747567035526 2316 6 -1.029914268442397 2316 7 -1.527002537987265 2316 8 -1.460029298211417 2316 9 -1.5324428313125438 2316 10 -1.5306864511201113 2316 11 -1.7154190785893348 2316 12 -1.9362012741606165 2316 13 -1.8909652070126413 2316 14 -1.9620605980910142 2316 15 -2.231494389980007 2316 16 -2.167171629057698 2316 17 -1.8960967743576873 2316 18 -2.107733877945549 2316 19 -1.9142676584990852 2316 20 -1.7921341547445166 2316 21 -2.2908043586191695 2316 22 -2.0157793912486923 2316 23 -2.2477511219453135 2316 24 -2.441518074593411 2316 25 -2.137566014756686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22083.0 3 0.0 4 179.0 5 836.0 6 957.0 7 890.0 8 785.0 9 750.0 10 742.0 11 686.0 12 671.0 13 675.0 14 611.0 15 667.0 16 705.0 17 683.0 18 904.0 19 1030.0 20 1364.0 21 1676.0 22 1950.0 23 2223.0 24 2645.0 25 3000.0 26 3419.0 27 3886.0 28 4597.0 29 5926.0 30 7328.0 31 10005.0 32 13737.0 33 20003.0 34 30770.0 35 49148.0 36 88585.0 37 122732.0 38 340143.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.31710957695608 18.62860462843595 13.378340569029614 25.675945225578356 2 16.32244405974791 20.980146489209076 39.46035590333614 23.237053547706875 3 17.808112815281575 24.676736399769208 30.75458740466753 26.76056338028169 4 11.770556807243997 17.275576278696796 39.308104113704175 31.645762800355026 5 12.714611019409872 37.4788986748167 36.392406334212865 13.414083971560567 6 29.8814845158777 37.99804816925505 18.806652288983404 13.313815025883846 7 26.04261630996893 32.55099459029627 23.428260849193634 17.978128250541168 8 24.188778713532024 35.658796424588786 21.718333955830794 18.4340909060484 9 24.937917592045956 15.934462396467964 21.286200235344204 37.84141977614188 10 14.073797408536382 28.849075825545423 35.02237644094775 22.05475032497045 11 32.625962026316245 23.45623976727966 25.0792847571122 18.838513449291895 12 21.732390350084536 25.287185521646176 32.3535357186365 20.62688840963278 13 27.101932955015524 22.344847528283474 26.907419232627973 23.645800284073033 14 20.439202078739903 21.723421031846435 29.297675607872115 28.539701281541547 15 22.328515336864836 29.376525286114557 25.551311863195135 22.74364751382547 16 21.678172829391517 28.42002112475251 27.442900918484963 22.45890512737101 17 20.88150995125778 27.883736216366728 28.761792310750735 22.47296152162476 18 20.60105141829018 27.645982347846225 29.831684719092998 21.921281514770595 19 21.41980291596552 27.34865614177413 29.90317152415491 21.32836941810544 20 21.582857089308973 26.956148066040956 29.989651816420814 21.471343028229256 21 21.856755971624825 26.754003729629943 29.669166027435406 21.720074271309826 22 21.433754133254794 27.12432562684909 29.66719768671602 21.774722553180094 23 21.12210337487784 26.976130871899223 29.452469243229494 22.44929650999344 24 20.937467787429835 26.927901407112 29.836370183844252 22.298260621613917 25 21.340417756037223 26.932185260598857 29.715351322840572 22.012045660523352 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 346.0 1 346.0 2 375.5 3 405.0 4 405.0 5 405.0 6 1094.5 7 1784.0 8 1784.0 9 1784.0 10 2443.0 11 3102.0 12 3102.0 13 3102.0 14 4309.0 15 5516.0 16 5516.0 17 5516.0 18 9142.0 19 12768.0 20 12768.0 21 12768.0 22 18492.5 23 24217.0 24 24217.0 25 24217.0 26 32284.5 27 40352.0 28 40352.0 29 40352.0 30 49411.0 31 58470.0 32 58470.0 33 58470.0 34 64731.5 35 70993.0 36 70993.0 37 70993.0 38 76963.5 39 82934.0 40 82934.0 41 82934.0 42 86659.5 43 90385.0 44 90385.0 45 90385.0 46 91860.5 47 93336.0 48 93336.0 49 93336.0 50 90302.0 51 87268.0 52 87268.0 53 87268.0 54 78434.5 55 69601.0 56 69601.0 57 69601.0 58 59600.0 59 49599.0 60 49599.0 61 49599.0 62 39618.5 63 29638.0 64 29638.0 65 29638.0 66 22636.0 67 15634.0 68 15634.0 69 15634.0 70 11249.5 71 6865.0 72 6865.0 73 6865.0 74 4726.0 75 2587.0 76 2587.0 77 2587.0 78 1727.0 79 867.0 80 867.0 81 867.0 82 548.0 83 229.0 84 229.0 85 229.0 86 148.5 87 68.0 88 68.0 89 68.0 90 44.0 91 20.0 92 20.0 93 20.0 94 13.0 95 6.0 96 6.0 97 6.0 98 3.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.264174534900688 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 1.338704214642479E-4 23 1.338704214642479E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 746991.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.24878493838375 #Duplication Level Percentage of deduplicated Percentage of total 1 78.96501196942522 54.68231131527625 2 13.43503184441717 18.60719261668763 3 4.066790066330319 8.448608120785908 4 1.5865269100611277 4.39460243175126 5 0.7276914079254176 2.519587290446846 6 0.37950284288297453 1.5768066450185005 7 0.23920438844579978 1.1595229276260555 8 0.14423819920113054 0.7990656029103075 9 0.10387627446867707 0.6473975211793747 >10 0.32796220336989346 3.709366975200707 >50 0.014537898659788717 0.7308242828192371 >100 0.008447301573952595 1.018383896162673 >500 1.9644887308505765E-4 0.12420311182217016 >1k 9.822443654252883E-4 1.5821272623130767 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4371 0.5851476122202276 No Hit TATCAACGCAGAGTACTTTTTTTTT 2581 0.34551955779922383 No Hit GGTATCAACGCAGAGTACTTTTTTT 2473 0.331061552281085 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1117 0.1495332607755649 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1088 0.1456510185531017 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 913 0.12222369479685832 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.338704214642479E-4 0.0 3 0.0 0.0 0.0 1.338704214642479E-4 0.0 4 0.0 0.0 0.0 1.338704214642479E-4 0.0 5 0.0 0.0 0.0 1.338704214642479E-4 0.0 6 0.0 0.0 0.0 1.338704214642479E-4 0.0 7 0.0 0.0 0.0 2.677408429284958E-4 0.0 8 0.0 0.0 0.0 2.677408429284958E-4 0.0 9 0.0 0.0 0.0 4.0161126439274366E-4 0.0 10 0.0 0.0 0.0 4.0161126439274366E-4 0.0 11 0.0 0.0 0.0 4.0161126439274366E-4 0.0 12 0.0 0.0 0.0 4.0161126439274366E-4 0.0 13 0.0 0.0 0.0 4.0161126439274366E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 35 0.0022348247 16.21273 5 TATGGAC 40 0.0054352493 14.18614 3 TAGGGTG 40 0.0054352493 14.18614 5 GCCTCGA 70 7.5946937E-6 13.510609 16 CTAAGGC 50 0.0015498443 13.240397 3 GGTATCA 480 0.0 13.170617 1 GTCTTAG 45 0.008885741 13.170617 1 ACGTGTC 60 4.245418E-4 12.609903 8 GGCGAGG 85 4.13819E-6 12.239023 19 TAGGACC 70 1.13607726E-4 12.159548 4 TCGAACT 70 1.13607726E-4 12.159548 19 GGCCTCG 90 7.827597E-6 11.559076 15 TGGCGAG 140 7.2941475E-10 11.484017 18 GTATAGC 95 8.801808E-6 11.437641 1 GACGTGG 75 2.1592698E-4 11.348912 7 GTTTAGG 80 2.6241757E-4 11.112708 1 GGACGTG 145 1.3042154E-9 11.088017 6 GTGAGCG 60 0.0060703605 11.033666 9 ATACTAT 95 1.4267354E-5 10.950705 6 GACGTGA 70 0.0015467008 10.808487 7 >>END_MODULE