FastQCFastQC Report
Thu 26 May 2016
SRR1511676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511676_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746991
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68380.9154059419725271No Hit
TATCAACGCAGAGTACTTTTTTTTT48320.6468618765152458No Hit
GGTATCAACGCAGAGTACTTTTTTT47370.6341441864761422No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22720.30415359756677124No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16490.22075232499454478No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15820.21178300675644018No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7970.10669472590700557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCA307.730226E-418.995389
CGAACCG551.9620503E-413.81482212
GGCGAAC707.2687053E-613.5681310
GGTATCA8900.013.4940421
GATCTAG500.001461346213.3441091
GAACCGG500.001501023413.29676613
GCGAACC655.457531E-513.15064811
CTACCCT604.0867526E-412.6669794
CATATAC1103.8002327E-812.0912073
GACTGTC550.003071469312.087977
TATTAGG752.0705913E-411.4002812
AGGCGAA752.0757486E-411.3972299
ACAGTCC600.0058814511.0806398
ACAAACC600.0058814511.0806398
TTAGGTT700.001489525110.857414
GCTCCAA700.00149285710.85450411
TATGGAC803.7648753E-410.6877633
GTATCAA19650.010.3803681
GTAACAC750.002644276510.1335833
GAGGCGA856.599139E-410.0563788
Produced by FastQC (version 0.11.5)