##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511676_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 746991 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.183788024219837 31.0 28.0 31.0 20.0 34.0 2 28.35769908874404 31.0 28.0 31.0 22.0 34.0 3 28.320193951466617 31.0 28.0 31.0 20.0 34.0 4 31.820514571126026 35.0 32.0 37.0 23.0 37.0 5 31.233621288609903 35.0 32.0 37.0 17.0 37.0 6 31.248420663702774 35.0 32.0 37.0 17.0 37.0 7 30.921811641639593 35.0 31.0 37.0 15.0 37.0 8 30.768048075545757 35.0 31.0 37.0 11.0 37.0 9 31.789954631314167 37.0 31.0 39.0 10.0 39.0 10 31.424209930240124 35.0 30.0 39.0 10.0 39.0 11 31.534188497585646 37.0 31.0 39.0 10.0 39.0 12 31.1645213931627 35.0 30.0 39.0 8.0 39.0 13 31.14991613018095 35.0 30.0 39.0 8.0 39.0 14 31.573946674056312 37.0 30.0 40.0 2.0 41.0 15 31.436883443040145 36.0 30.0 40.0 2.0 41.0 16 31.201066679518227 36.0 30.0 39.0 2.0 41.0 17 31.141165020729836 36.0 30.0 39.0 2.0 40.0 18 30.987291680890397 36.0 29.0 39.0 2.0 40.0 19 31.020800786087115 36.0 29.0 39.0 2.0 40.0 20 31.07847350235813 37.0 30.0 40.0 2.0 41.0 21 30.970278089026507 37.0 30.0 40.0 2.0 41.0 22 30.746600695322968 36.0 29.0 40.0 2.0 41.0 23 30.526709157138438 36.0 29.0 39.0 2.0 41.0 24 30.148956279259053 36.0 29.0 39.0 2.0 41.0 25 29.45208442939741 36.0 27.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1254182944223068 1101 2 0.49281609801218096 1101 3 0.40221749990571 1101 4 0.2719969180271953 1101 5 0.40559365442134876 1101 6 -0.010439442095215412 1101 7 0.2945787015695416 1101 8 0.15073452279590427 1101 9 0.4861169725098726 1101 10 0.46813255817698973 1101 11 0.3624850677454887 1101 12 0.7434759743980237 1101 13 0.7201319995891495 1101 14 0.7726988663373113 1101 15 0.7378924486841036 1101 16 0.3040427215009558 1101 17 0.5131803707332985 1101 18 0.5036877700973363 1101 19 0.060442130921714465 1101 20 -0.17078568824688745 1101 21 0.16465524396053155 1101 22 0.4914646522908299 1101 23 0.5328975179830806 1101 24 0.03745816690853587 1101 25 0.24951945401769748 1102 1 0.5159655581039004 1102 2 0.5134120430183344 1102 3 0.3345763151484924 1102 4 0.5728448290099131 1102 5 0.5703835044543375 1102 6 0.941534558867712 1102 7 0.9205661478506819 1102 8 0.7245143690841758 1102 9 0.8392794754075688 1102 10 1.0825158389059588 1102 11 0.7748586835374986 1102 12 1.124264683183842 1102 13 1.266446127872463 1102 14 1.195160928784933 1102 15 0.7924137489925975 1102 16 0.8086389161549263 1102 17 1.002842276259429 1102 18 1.1810313389912181 1102 19 1.4927608241937058 1102 20 0.9031992258920951 1102 21 0.7392152396808562 1102 22 0.36712220681213026 1102 23 1.1299748553107811 1102 24 1.113386767632516 1102 25 1.3687441192221428 1103 1 0.6502581240511418 1103 2 1.0403840138287812 1103 3 0.9291243326484704 1103 4 1.054872110410603 1103 5 1.5084158742574196 1103 6 1.4684162792978164 1103 7 1.0624569362951952 1103 8 1.182840181552482 1103 9 1.7333341241616829 1103 10 1.9365637833461058 1103 11 1.182145757104454 1103 12 1.3973021671751447 1103 13 1.4834467209463895 1103 14 1.4291199980886269 1103 15 1.7462141495323813 1103 16 1.5674469235124775 1103 17 1.8017644288599328 1103 18 1.5831913314273791 1103 19 1.5711798094356162 1103 20 1.8501706809860856 1103 21 1.694747587995753 1103 22 1.770214786511815 1103 23 1.549415646763638 1103 24 1.714755135303971 1103 25 1.5523219881098278 1104 1 0.46524092042274034 1104 2 0.40530077607116155 1104 3 0.529696049188285 1104 4 0.6562171213583632 1104 5 0.5328919455742813 1104 6 0.7860491295393643 1104 7 0.825168506169728 1104 8 0.8836100004799476 1104 9 0.8717972666911429 1104 10 0.9494578159461149 1104 11 1.120308270571936 1104 12 1.2343368872251936 1104 13 0.7216822195040677 1104 14 1.178694251729194 1104 15 1.0984627854062872 1104 16 1.0918969287257028 1104 17 1.2406481045280913 1104 18 1.31292059149758 1104 19 0.7889012844295387 1104 20 1.0654245648355278 1104 21 1.382623374323213 1104 22 1.0247763546486048 1104 23 1.0097343483241303 1104 24 1.2518211346783836 1104 25 1.250763235112224 1105 1 0.35847881799782044 1105 2 0.6084314118956407 1105 3 0.7438219013988565 1105 4 0.6190362014144206 1105 5 0.6505058742280703 1105 6 0.6303909835001846 1105 7 0.9139314482401062 1105 8 0.8399262629933268 1105 9 0.7168117239667886 1105 10 0.5935397937250748 1105 11 0.7754904204190858 1105 12 0.7359600046750359 1105 13 0.6832433096240429 1105 14 0.7326710373062149 1105 15 0.6444703303894066 1105 16 0.5503433481108679 1105 17 0.9171717358173055 1105 18 0.9248210875979623 1105 19 0.9228010827963686 1105 20 0.8866687774905202 1105 21 0.8747842196451465 1105 22 0.598694719823424 1105 23 1.0603185523461498 1105 24 1.088427311394188 1105 25 1.5768546263810954 1106 1 -0.31079668801318405 1106 2 -0.1221913022624328 1106 3 -0.12778927624935577 1106 4 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2313 24 -0.35390469093340116 2313 25 -0.2137176392047131 2314 1 -0.27114928783447567 2314 2 -0.17527203276251768 2314 3 -0.26349804004161115 2314 4 -0.3383024255908502 2314 5 -0.6942119394579933 2314 6 -0.49937083554812034 2314 7 -0.7911794823010219 2314 8 -0.6676525335191599 2314 9 -0.8675767437826138 2314 10 -1.8316082336621342 2314 11 -1.0601914655543965 2314 12 -0.9090074070142435 2314 13 -0.7543966178279682 2314 14 -1.075152214393487 2314 15 -0.7072348559691015 2314 16 -0.7716397903097381 2314 17 -0.5414937377160527 2314 18 -0.45558101204238355 2314 19 -0.26149858566342843 2314 20 -0.6699913406571056 2314 21 -0.52202689158025 2314 22 -0.40690066398493485 2314 23 -0.40593771760368824 2314 24 -0.460576868000949 2314 25 -0.7639426472407145 2315 1 -1.1119080051144898 2315 2 -1.0379567618265568 2315 3 -1.1279334693857628 2315 4 -1.447980779691111 2315 5 -1.6422704732163247 2315 6 -1.5215262494378123 2315 7 -1.315302924781463 2315 8 -1.6483948304379261 2315 9 -1.6245795449030638 2315 10 -1.713502370627264 2315 11 -1.5931891473857505 2315 12 -1.7614777054784554 2315 13 -1.9189141489783594 2315 14 -1.4762074640795682 2315 15 -1.5537527734809338 2315 16 -1.6658491982108288 2315 17 -1.9107345374842346 2315 18 -1.9111132249275364 2315 19 -1.551969946621604 2315 20 -1.7427963247196985 2315 21 -1.977800580366111 2315 22 -2.0848563290095647 2315 23 -1.5751398812277593 2315 24 -1.9219045025030255 2315 25 -1.8455011327038662 2316 1 -0.9118287050019376 2316 2 -1.1667938640469586 2316 3 -1.0249456457901402 2316 4 -1.2017581936547508 2316 5 -1.502318564897486 2316 6 -1.2783690817308653 2316 7 -1.7278425748377408 2316 8 -2.1366490341965836 2316 9 -1.897823857464882 2316 10 -1.8784210240601915 2316 11 -2.0414522191139817 2316 12 -2.1016197635295484 2316 13 -2.194733583474761 2316 14 -2.151592879680372 2316 15 -1.9367978978030465 2316 16 -1.8566043399278058 2316 17 -2.0589404790238532 2316 18 -2.295509861920614 2316 19 -2.3645914548585836 2316 20 -2.092748574114289 2316 21 -2.0664333782105118 2316 22 -1.9211015965834939 2316 23 -1.9537936552309674 2316 24 -2.21687107707924 2316 25 -2.1218876056395075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40713.0 3 0.0 4 594.0 5 4638.0 6 5527.0 7 4649.0 8 3735.0 9 3383.0 10 3075.0 11 2866.0 12 2767.0 13 2801.0 14 2665.0 15 2640.0 16 2704.0 17 2712.0 18 2971.0 19 3380.0 20 4108.0 21 5299.0 22 6503.0 23 8074.0 24 9521.0 25 10808.0 26 12566.0 27 14449.0 28 16565.0 29 19794.0 30 24468.0 31 30266.0 32 37838.0 33 47018.0 34 58683.0 35 73585.0 36 92434.0 37 105932.0 38 77260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.26623708154414 23.482971553869223 16.090363355923277 19.16042800866336 2 18.49018261264192 25.28156296394468 36.924139648268856 19.304114775144544 3 21.18191517702355 28.076911234539644 31.128889103081566 19.612284485355243 4 16.64103048095626 21.130241194338353 36.68424385300492 25.544484471700464 5 13.250709732711982 39.86715946221008 35.3321013444748 11.55002946060314 6 30.155383398193553 40.46902840864214 17.506368885301164 11.869219307863146 7 26.44315661098996 34.34365340412401 22.94257896012134 16.27061102476469 8 25.837928435550094 37.15908223793861 20.54214843284591 16.460840893665384 9 27.038879986505858 16.368470302855055 20.645898009480703 35.946751701158384 10 15.382648519192333 31.193013034962934 34.006299942034104 19.41803850381062 11 34.85530615496037 24.101227457894407 23.824785037570734 17.218681349574496 12 22.992111016063113 27.166860109425684 31.432908830226868 18.408120044284335 13 29.4239154153129 23.19438922289559 26.278763733431866 21.102931628359645 14 21.884868760132317 22.94405153475745 29.038904083181727 26.13217562192851 15 23.796806119484707 30.595013862282144 25.15746508324732 20.450714934985832 16 22.60187873749483 29.66274024720512 27.13968441386844 20.59569660143161 17 21.76077087943496 29.161797130085905 28.29819904122004 20.779232949259093 18 21.56947004716255 28.13527873829805 30.011606565540948 20.283644648998447 19 22.788761845858918 28.042372665801864 30.109465843631316 19.059399644707902 20 22.847129349617333 27.343836806601416 30.357126123340173 19.451907720441074 21 23.258513154776967 27.21558894283867 30.005850137417987 19.520047764966378 22 22.734410454744435 27.489621695575984 30.142264096890052 19.633703752789526 23 22.52731291273924 27.515190946075656 29.915353732508155 20.042142408676945 24 22.368542592882644 27.46793468729878 30.10370941550835 20.059813304310225 25 22.59029692896731 27.488955527591102 30.191571394147097 19.729176149294492 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 356.0 1 356.0 2 545.5 3 735.0 4 735.0 5 735.0 6 1612.0 7 2489.0 8 2489.0 9 2489.0 10 3363.5 11 4238.0 12 4238.0 13 4238.0 14 5471.5 15 6705.0 16 6705.0 17 6705.0 18 10886.5 19 15068.0 20 15068.0 21 15068.0 22 21932.5 23 28797.0 24 28797.0 25 28797.0 26 38532.0 27 48267.0 28 48267.0 29 48267.0 30 56507.0 31 64747.0 32 64747.0 33 64747.0 34 70876.5 35 77006.0 36 77006.0 37 77006.0 38 80449.5 39 83893.0 40 83893.0 41 83893.0 42 86172.0 43 88451.0 44 88451.0 45 88451.0 46 87877.5 47 87304.0 48 87304.0 49 87304.0 50 83938.0 51 80572.0 52 80572.0 53 80572.0 54 72323.0 55 64074.0 56 64074.0 57 64074.0 58 54072.5 59 44071.0 60 44071.0 61 44071.0 62 35068.5 63 26066.0 64 26066.0 65 26066.0 66 20128.5 67 14191.0 68 14191.0 69 14191.0 70 10263.5 71 6336.0 72 6336.0 73 6336.0 74 4389.5 75 2443.0 76 2443.0 77 2443.0 78 1647.5 79 852.0 80 852.0 81 852.0 82 560.5 83 269.0 84 269.0 85 269.0 86 158.0 87 47.0 88 47.0 89 47.0 90 30.0 91 13.0 92 13.0 93 13.0 94 7.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36238723090371905 2 0.0 3 0.0 4 0.0 5 0.030924067358241263 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.001472574636106727 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 746991.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.55525460060996 #Duplication Level Percentage of deduplicated Percentage of total 1 82.857614959899 59.2889773405488 2 10.862671321182958 15.545624240599812 3 3.2894038167509825 7.0612238277550405 4 1.3235408870184797 3.7882522057969776 5 0.6057458774977903 2.1672150243812136 6 0.33338561504305064 1.4313295538751858 7 0.19416347304792525 0.9725391723658071 8 0.11892884349363636 0.6807986940434595 9 0.08414347635590495 0.5418817086264464 >10 0.30703544906485636 3.532142200984049 >50 0.01364616962477614 0.6709394323167849 >100 0.007850858908340641 1.0168040745243583 >500 7.477008449504792E-4 0.37170461925156906 >1k 9.346260561880989E-4 2.015952508585696 >5k 1.869252112376198E-4 0.9146153963448108 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6838 0.9154059419725271 No Hit TATCAACGCAGAGTACTTTTTTTTT 4832 0.6468618765152458 No Hit GGTATCAACGCAGAGTACTTTTTTT 4737 0.6341441864761422 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2272 0.30415359756677124 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1649 0.22075232499454478 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1582 0.21178300675644018 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 797 0.10669472590700557 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.338704214642479E-4 0.0 3 0.0 0.0 0.0 1.338704214642479E-4 0.0 4 0.0 0.0 0.0 1.338704214642479E-4 0.0 5 0.0 0.0 0.0 1.338704214642479E-4 0.0 6 0.0 0.0 0.0 1.338704214642479E-4 0.0 7 0.0 0.0 0.0 4.0161126439274366E-4 0.0 8 0.0 0.0 0.0 4.0161126439274366E-4 0.0 9 0.0 0.0 0.0 6.693521073212395E-4 0.0 10 0.0 0.0 0.0 6.693521073212395E-4 0.0 11 0.0 0.0 0.0 6.693521073212395E-4 0.0 12 0.0 0.0 0.0 6.693521073212395E-4 0.0 13 0.0 0.0 0.0 6.693521073212395E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGATCA 30 7.730226E-4 18.99538 9 CGAACCG 55 1.9620503E-4 13.814822 12 GGCGAAC 70 7.2687053E-6 13.56813 10 GGTATCA 890 0.0 13.494042 1 GATCTAG 50 0.0014613462 13.344109 1 GAACCGG 50 0.0015010234 13.296766 13 GCGAACC 65 5.457531E-5 13.150648 11 CTACCCT 60 4.0867526E-4 12.666979 4 CATATAC 110 3.8002327E-8 12.091207 3 GACTGTC 55 0.0030714693 12.08797 7 TATTAGG 75 2.0705913E-4 11.400281 2 AGGCGAA 75 2.0757486E-4 11.397229 9 ACAGTCC 60 0.00588145 11.080639 8 ACAAACC 60 0.00588145 11.080639 8 TTAGGTT 70 0.0014895251 10.85741 4 GCTCCAA 70 0.001492857 10.854504 11 TATGGAC 80 3.7648753E-4 10.687763 3 GTATCAA 1965 0.0 10.380368 1 GTAACAC 75 0.0026442765 10.133583 3 GAGGCGA 85 6.599139E-4 10.056378 8 >>END_MODULE