FastQCFastQC Report
Wed 25 May 2016
SRR1295366_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295366_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491354
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGGAGGCTGAGGCAGGAGAAT131480.37658742138436835No Hit
ATTCCATTCCATTCCATTCCATTCC111170.31841514781944197No Hit
GTGGTATCAACGCAGAGTACATGGG86250.24703882791604634No Hit
GTACATGGGAAGCAGTGGTATCAAC78150.22383865972914807No Hit
GAATGGAATGGAATGGAATGGAATG72860.20868694495029722No Hit
CTGTCACCCAGGCTGGAGTGCAGTG54440.15592804396231377No Hit
ACTTGGGAGGCTGAGGCAGGAGAAT49350.14134917284239867No Hit
CCCCATCTCTACTAAAAATACAAAA48640.13931557785317675No Hit
CCCATGTACTCTGCGTTGATACCAC48350.1384849545477199No Hit
ACTCGGGAGGCTGAGGCAGGAGAAT44550.12760092502794043No Hit
GTATCAACGCAGAGTACTTTTTTTT43880.12568189877050565No Hit
CTGTCGCCCAGGCTGGAGTGCAGTG43250.123877441244858No Hit
GGTATCAACGCAGAGTACATGGGGG41180.11794850937487289No Hit
GTACATAAGCAGTGGTATCAACGCA40240.11525614417787482No Hit
CCTGTAATCCCAGCACTTTGGGAGG37860.1084393046365393No Hit
GTTCAAGACCAGCCTGGCCAACATG37750.10812424062412462No Hit
GCTTATGTACTCTGCGTTGATACCA37540.10752275478224206No Hit
GTCTCGCTCTGTCGCCCAGGCTGGA36330.10405705064568073No Hit
GGAATGGAATGGAATGGAATGGAAT35440.10150789636341659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC2650.015.4150933
TATAACG2650.015.4150932
GCGTAGC653.3750366E-614.6153844
AGACGTA500.001500200513.2999992
GTATAAC3150.013.2698411
TATCACG2900.013.1034482
CGGAGTT1900.013.05
ACAACGC1207.421477E-1012.6666673
GAGACGA1155.333277E-912.3913041
GTCTTAT853.9472907E-612.2941181
GTCGTAC550.003069762612.0909091
CGGTATC550.003069762612.0909092
TAACGCA3650.011.9726034
TGCGGAC2000.011.8759
TACAACG1201.0029908E-811.8752
ACTCCGG4350.011.7931031
CCGGACT2350.011.7234043
GCGCACT658.0278213E-411.69230713
GCCGGAC2400.011.4791672
CTAGACC1001.9316576E-611.44
Produced by FastQC (version 0.11.5)