FastQCFastQC Report
Wed 25 May 2016
SRR1295366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295366_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491354
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT455481.304594148860299No Hit
TATCAACGCAGAGTACTTTTTTTTT335470.9608593113158963No Hit
GGTATCAACGCAGAGTACTTTTTTT310960.8906573209133191No Hit
GTACTTTTTTTTTTTTTTTTTTTTT292250.8370677966198787No Hit
GAGTACTTTTTTTTTTTTTTTTTTT138830.3976394258502576No Hit
ACTCAGGAGGCTGAGGCAGGAGAAT128550.36819526178095946No Hit
ACGCAGAGTACTTTTTTTTTTTTTT119170.34132889417687234No Hit
ATTCCATTCCATTCCATTCCATTCC111730.3200191100644621No Hit
GTGGTATCAACGCAGAGTACATGGG83570.23936272288630714No Hit
GTACATGGGAAGCAGTGGTATCAAC81270.2327750208085459No Hit
GAATGGAATGGAATGGAATGGAATG71330.20430469095943865No Hit
GTGGTATCAACGCAGAGTACTTTTT58590.16781454988523078No Hit
ATCAACGCAGAGTACTTTTTTTTTT55970.1603102979531723No Hit
CTGTCACCCAGGCTGGAGTGCAGTG54510.15612853924294126No Hit
CCCATGTACTCTGCGTTGATACCAC50340.1441847489541307No Hit
GCAGAGTACTTTTTTTTTTTTTTTT47150.13504789259410532No Hit
ACTTGGGAGGCTGAGGCAGGAGAAT47130.13499060822821174No Hit
CCCCATCTCTACTAAAAATACAAAA45840.1312957666280761No Hit
GTACATAAGCAGTGGTATCAACGCA44360.12705672355195147No Hit
CTGTCGCCCAGGCTGGAGTGCAGTG43840.1255673300387185No Hit
ACTCGGGAGGCTGAGGCAGGAGAAT40170.11505564889724733No Hit
CCTGTAATCCCAGCACTTTGGGAGG36720.10517409578060545No Hit
GCTTATGTACTCTGCGTTGATACCA36430.10434347247514861No Hit
GGTATCAACGCAGAGTACATGGGGG36020.1031691429743303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3450.016.521742
TTAGACT350.002170928316.2857154
TATAACG2900.015.7241372
ATAACGC3050.015.2622963
TGCGGAC2050.014.82926759
GCGGACT2100.014.476191510
TACGACC400.005280848614.254
GGTTTAT2350.014.1489351
GTATAAC3300.014.106061
ACAACGC2100.014.023813
GCGTATC707.268749E-613.5714281
TAACGCA3650.013.5342474
GTATCAC5050.012.2277221
ATAGAAC701.0916517E-412.2142863
CACGCAG5100.012.1078445
TCTAGAC1501.4551915E-1112.0333333
GCGGGAT2300.011.9782612
GACTGCG2550.011.921576
CTAGACC1302.6193447E-911.6923074
CTGCGGA2600.011.6923078
Produced by FastQC (version 0.11.5)