##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295366_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3491354 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45054497481493 33.0 32.0 34.0 32.0 34.0 2 32.57721416963161 33.0 32.0 34.0 32.0 34.0 3 32.68468479564089 33.0 32.0 34.0 32.0 34.0 4 32.823530355271906 33.0 32.0 34.0 32.0 34.0 5 32.573197103473326 33.0 32.0 34.0 32.0 34.0 6 35.34893482585839 37.0 34.0 37.0 33.0 37.0 7 35.25745770838477 37.0 34.0 37.0 33.0 37.0 8 35.16344002928377 37.0 34.0 37.0 32.0 37.0 9 35.1292091836004 37.0 34.0 37.0 32.0 37.0 10 34.99164135175064 37.0 34.0 37.0 32.0 37.0 11 35.057107070781136 37.0 34.0 37.0 32.0 37.0 12 34.98748307963042 37.0 34.0 37.0 32.0 37.0 13 36.77297690237083 38.0 38.0 38.0 35.0 38.0 14 36.78105027447804 38.0 38.0 38.0 35.0 38.0 15 36.650349978833425 38.0 38.0 38.0 34.0 38.0 16 36.782570028705194 38.0 38.0 38.0 35.0 38.0 17 36.78643070854459 38.0 38.0 38.0 35.0 38.0 18 36.89452974404772 38.0 38.0 38.0 36.0 38.0 19 36.8594780134011 38.0 38.0 38.0 36.0 38.0 20 36.865743204498884 38.0 38.0 38.0 36.0 38.0 21 36.826222720468905 38.0 38.0 38.0 35.0 38.0 22 36.837375986508384 38.0 38.0 38.0 36.0 38.0 23 37.36087460624159 39.0 38.0 39.0 36.0 39.0 24 37.313569463308504 39.0 38.0 39.0 35.0 39.0 25 37.21594945685828 39.0 38.0 39.0 35.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08786939123577753 1101 2 0.33506954024799285 1101 3 0.3765231127053923 1101 4 0.20736451439239545 1101 5 0.38025481015278473 1101 6 0.13908431598326843 1101 7 0.17986481555917777 1101 8 0.1880400404090139 1101 9 0.042819582321918404 1101 10 0.26664112541914164 1101 11 0.17984442691337676 1101 12 0.14622197386875513 1101 13 0.20237056739389914 1101 14 0.1843376835333217 1101 15 0.510837696483641 1101 16 0.2202041397744665 1101 17 0.3485992912529241 1101 18 0.1847764145105515 1101 19 0.2590266443453402 1101 20 0.27714957425554587 1101 21 0.3763035568685993 1101 22 0.2916578067356781 1101 23 0.3100422401937948 1101 24 0.19518673118462715 1101 25 0.3135712816570617 1102 1 -0.030297520348511853 1102 2 0.025337799739340028 1102 3 0.16547336681308167 1102 4 0.17766899996126995 1102 5 0.286817923295672 1102 6 0.027296218164707398 1102 7 0.22777049828175677 1102 8 0.17111218109766213 1102 9 0.10442494262244395 1102 10 4.4054920629577055E-4 1102 11 0.10098955486359529 1102 12 0.1591832089779004 1102 13 0.15095552302351933 1102 14 0.21587893064840813 1102 15 0.3884454353371751 1102 16 -0.019818862562438255 1102 17 0.2170662156037082 1102 18 0.007890073489249971 1102 19 0.1899748829756902 1102 20 0.1113335322872544 1102 21 0.2680318830173789 1102 22 0.33760383160047525 1102 23 0.24575546203302423 1102 24 0.20695391367225113 1102 25 0.1663861139767704 1103 1 0.24252287424238972 1103 2 0.2168574172042952 1103 3 0.21391147838890134 1103 4 0.19467395818973188 1103 5 0.37928243810589635 1103 6 0.24922665197775729 1103 7 0.16960454919738766 1103 8 0.1417855489415345 1103 9 0.1646570525234523 1103 10 0.06706103857148804 1103 11 0.042106265452410696 1103 12 0.1912326274981453 1103 13 0.18815315413085898 1103 14 0.11968561084209739 1103 15 0.2096012403204881 1103 16 0.11983427631325583 1103 17 0.17397997680734534 1103 18 0.19005740818302996 1103 19 0.2226852244070372 1103 20 0.21810916809381808 1103 21 0.2040893480606485 1103 22 0.341414518795375 1103 23 0.17195094213565199 1103 24 0.08451615866146511 1103 25 0.04072405865228035 1104 1 0.1830196517732432 1104 2 0.2885527395466383 1104 3 0.1531072136228815 1104 4 0.20120714246633042 1104 5 0.15846452572663594 1104 6 0.15973745477194967 1104 7 0.14490264767982808 1104 8 0.1684912061922219 1104 9 0.19754784104583933 1104 10 0.23402467202720345 1104 11 0.25932043871282673 1104 12 0.2582468869509569 1104 13 0.2797068675480361 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-0.08458037063890345 2110 10 0.0048056496738340115 2110 11 0.10245537381918979 2110 12 -0.09209374508652246 2110 13 -0.34481301204635884 2110 14 -0.26588713366124495 2110 15 0.0372943679874993 2110 16 -0.0986413238670707 2110 17 -0.15464578294174203 2110 18 -0.05653034622851294 2110 19 -0.023929258770031936 2110 20 -0.07057078266743844 2110 21 -0.2440235441086145 2110 22 -0.5678005860387856 2110 23 -0.16611389205702665 2110 24 -0.06205151734081937 2110 25 -0.08422368798003532 2111 1 0.03407892759346964 2111 2 -0.04409117290809661 2111 3 -0.21177109798723848 2111 4 -0.12027165231545922 2111 5 -0.21231537248507237 2111 6 -0.03539853058983766 2111 7 -0.07361602301028114 2111 8 -0.366071983467414 2111 9 -0.1691624453738143 2111 10 -0.3421741586810967 2111 11 -0.16107923375404454 2111 12 -0.01858859574897309 2111 13 -0.8599242208089493 2111 14 -0.26128139814687756 2111 15 0.04025662459635271 2111 16 -0.22514367118589007 2111 17 -0.03386901741078674 2111 18 -0.36052358826844255 2111 19 0.015677054279301217 2111 20 0.024953094361457318 2111 21 -0.0018511471345235009 2111 22 -0.08345988461017129 2111 23 -0.0024544752293067518 2111 24 -0.03438583320765076 2111 25 0.007423670564072893 2112 1 -0.16501281975266124 2112 2 -0.05468471719373724 2112 3 -0.15213154645795868 2112 4 -0.19946638092706337 2112 5 -0.5159298790148341 2112 6 -0.11429119320225567 2112 7 -0.1039611259637141 2112 8 -0.15646327578240005 2112 9 -0.20503628600909707 2112 10 -0.16681346244089212 2112 11 -0.2642625619174197 2112 12 -0.17518234926855314 2112 13 -0.06346996584391462 2112 14 -0.39526610194707246 2112 15 -0.1426533718073557 2112 16 -0.5698084054010124 2112 17 -0.3953256308963162 2112 18 -0.6997149344343327 2112 19 -0.2926764009666485 2112 20 -0.2783361990473239 2112 21 -0.13822256921795883 2112 22 -0.21671789344087955 2112 23 -0.23623930925660375 2112 24 -0.4037107702180691 2112 25 -0.10356630561459212 2113 1 -0.1923351956259225 2113 2 -0.34410463167856165 2113 3 -0.3268020337166391 2113 4 -0.366118000293163 2113 5 -0.4928282201521128 2113 6 -0.1443887292852466 2113 7 -0.4911521232123235 2113 8 -0.26977125514802225 2113 9 -0.29113409517857036 2113 10 -0.19775044147890952 2113 11 -0.459227240721674 2113 12 -0.2707384197209848 2113 13 -0.10822930713021606 2113 14 -0.07915164411915043 2113 15 -0.16537583418057977 2113 16 -0.22884571816851462 2113 17 -0.20727320560446572 2113 18 -0.3209818746977291 2113 19 -0.15400675527312302 2113 20 -0.4228239913067071 2113 21 -0.2862833108243379 2113 22 -0.13450935429084154 2113 23 -0.09734141418769582 2113 24 -0.5658627613190959 2113 25 -0.059961847468564144 2114 1 -0.7910314730947228 2114 2 -0.36833846629537703 2114 3 -0.42810184896733006 2114 4 -0.3717282490212668 2114 5 -0.531809157754779 2114 6 -0.17417626817975673 2114 7 -0.23310321222231778 2114 8 -0.3207601829979225 2114 9 -0.28302606384226436 2114 10 -0.3386379824705017 2114 11 -0.4723867837128921 2114 12 -0.25535567048164154 2114 13 -0.173153682261308 2114 14 -0.22027697565714988 2114 15 -0.40050649634825675 2114 16 -0.3948976003079636 2114 17 -0.25801555051664593 2114 18 -0.42186012387724503 2114 19 -0.3092240596689848 2114 20 -0.7294453448331311 2114 21 -0.32611649621033223 2114 22 -0.2565301367401318 2114 23 -0.34755485797221297 2114 24 -0.5505847207325942 2114 25 -0.16999079407341355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 3.0 16 8.0 17 7.0 18 10.0 19 3.0 20 19.0 21 65.0 22 260.0 23 898.0 24 2462.0 25 5410.0 26 11029.0 27 19094.0 28 30668.0 29 45293.0 30 63112.0 31 83679.0 32 108840.0 33 148413.0 34 233050.0 35 547898.0 36 1814372.0 37 376761.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.51435632135842 18.281159687616896 9.534381217143837 21.67010277388085 2 23.181235703970437 20.080117914138757 34.3427793343213 22.39586704756951 3 19.201518952246033 24.957366110683708 28.34791315919268 27.49320177787758 4 15.941150625230211 17.309989190440156 32.609325780198745 34.13953440413089 5 13.449337993225551 29.963561414855093 28.083660379325615 28.503440212593738 6 33.30535946798864 32.81887199063745 16.876862099918828 16.99890644145509 7 26.963665099557367 28.802894235302407 21.626910361997094 22.606530303143135 8 25.53739895753911 27.045352605321604 24.90085508372969 22.516393353409594 9 24.753576979017307 14.22390854665554 23.470636320464784 37.551878153862376 10 21.751647068730353 21.882799624443695 28.467236493348995 27.89831681347695 11 28.95507015329869 23.928510257052135 20.78428598188554 26.332133607763637 12 27.539516187702535 23.753850225442623 28.13888250804702 20.56775107880782 13 29.086537773024446 20.234384711490154 25.998137112421144 24.680940403064255 14 25.513597303510327 21.651857703343747 25.465020161232577 27.36952483191335 15 27.83762402781271 21.75448264484209 26.381083098419694 24.02681022892551 16 26.16669635906299 26.058658044987702 21.25312987454151 26.521515721407795 17 19.94292185782364 27.39066276292808 25.985362698826876 26.68105268042141 18 19.797705990283426 24.747533478415537 28.96684209048982 26.487918440811214 19 23.036678606638 19.880882889560898 31.142158600932472 25.940279902868628 20 28.87676242512217 22.248187952295872 26.973317515210432 21.901732107371526 21 27.429845269199284 23.40919883804392 23.515031704032303 25.64592418872449 22 24.44412683446021 25.034614078091195 28.17047483583733 22.350784251611266 23 23.931517686261547 28.07180251558564 26.18860762901728 21.808072169135528 24 22.093462880017324 23.241097866329223 29.53862598865655 25.126813264996905 25 24.078308873863836 21.583316959552082 27.966141502694942 26.37223266388914 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1124.0 1 1124.0 2 2962.5 3 4801.0 4 4801.0 5 4801.0 6 18071.5 7 31342.0 8 31342.0 9 31342.0 10 25349.0 11 19356.0 12 19356.0 13 19356.0 14 14198.0 15 9040.0 16 9040.0 17 9040.0 18 15451.5 19 21863.0 20 21863.0 21 21863.0 22 33188.5 23 44514.0 24 44514.0 25 44514.0 26 71008.5 27 97503.0 28 97503.0 29 97503.0 30 121418.0 31 145333.0 32 145333.0 33 145333.0 34 168555.0 35 191777.0 36 191777.0 37 191777.0 38 219769.0 39 247761.0 40 247761.0 41 247761.0 42 254966.5 43 262172.0 44 262172.0 45 262172.0 46 293234.0 47 324296.0 48 324296.0 49 324296.0 50 389425.0 51 454554.0 52 454554.0 53 454554.0 54 503028.5 55 551503.0 56 551503.0 57 551503.0 58 520624.0 59 489745.0 60 489745.0 61 489745.0 62 415425.5 63 341106.0 64 341106.0 65 341106.0 66 253988.5 67 166871.0 68 166871.0 69 166871.0 70 112730.0 71 58589.0 72 58589.0 73 58589.0 74 39335.0 75 20081.0 76 20081.0 77 20081.0 78 13014.5 79 5948.0 80 5948.0 81 5948.0 82 3763.0 83 1578.0 84 1578.0 85 1578.0 86 983.5 87 389.0 88 389.0 89 389.0 90 240.5 91 92.0 92 92.0 93 92.0 94 52.0 95 12.0 96 12.0 97 12.0 98 8.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3491354.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.806891909522555 #Duplication Level Percentage of deduplicated Percentage of total 1 80.88813662512925 33.00793448024342 2 9.582101317013231 7.820315454189054 3 3.154118092755767 3.8612926824286213 4 1.558684317910176 2.5442024992811376 5 0.9507159261164694 1.93978810168482 6 0.6411111376945355 1.5697051738735166 7 0.46809785547598826 1.3371133014041574 8 0.3502589805802771 1.143438428870314 9 0.2906363312813789 1.0673968820021473 >10 1.7799077026780408 13.987544129466123 >50 0.1842098146494486 5.218272714012661 >100 0.13092863520998282 10.499606804046866 >500 0.012234092833203614 3.489138323624827 >1k 0.007804507496943584 5.7773862079528175 >5k 4.921761484559016E-4 1.3071357455610293 >10k+ 5.624870268067447E-4 5.42972907135852 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 45548 1.304594148860299 No Hit TATCAACGCAGAGTACTTTTTTTTT 33547 0.9608593113158963 No Hit GGTATCAACGCAGAGTACTTTTTTT 31096 0.8906573209133191 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 29225 0.8370677966198787 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 13883 0.3976394258502576 No Hit ACTCAGGAGGCTGAGGCAGGAGAAT 12855 0.36819526178095946 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 11917 0.34132889417687234 No Hit ATTCCATTCCATTCCATTCCATTCC 11173 0.3200191100644621 No Hit GTGGTATCAACGCAGAGTACATGGG 8357 0.23936272288630714 No Hit GTACATGGGAAGCAGTGGTATCAAC 8127 0.2327750208085459 No Hit GAATGGAATGGAATGGAATGGAATG 7133 0.20430469095943865 No Hit GTGGTATCAACGCAGAGTACTTTTT 5859 0.16781454988523078 No Hit ATCAACGCAGAGTACTTTTTTTTTT 5597 0.1603102979531723 No Hit CTGTCACCCAGGCTGGAGTGCAGTG 5451 0.15612853924294126 No Hit CCCATGTACTCTGCGTTGATACCAC 5034 0.1441847489541307 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 4715 0.13504789259410532 No Hit ACTTGGGAGGCTGAGGCAGGAGAAT 4713 0.13499060822821174 No Hit CCCCATCTCTACTAAAAATACAAAA 4584 0.1312957666280761 No Hit GTACATAAGCAGTGGTATCAACGCA 4436 0.12705672355195147 No Hit CTGTCGCCCAGGCTGGAGTGCAGTG 4384 0.1255673300387185 No Hit ACTCGGGAGGCTGAGGCAGGAGAAT 4017 0.11505564889724733 No Hit CCTGTAATCCCAGCACTTTGGGAGG 3672 0.10517409578060545 No Hit GCTTATGTACTCTGCGTTGATACCA 3643 0.10434347247514861 No Hit GGTATCAACGCAGAGTACATGGGGG 3602 0.1031691429743303 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.864218294678798E-5 0.0 6 0.0 0.0 0.0 2.864218294678798E-5 0.0 7 0.0 0.0 0.0 2.864218294678798E-5 0.0 8 0.0 0.0 0.0 2.864218294678798E-5 0.0 9 0.0 0.0 0.0 5.728436589357596E-5 0.0 10 0.0 0.0 0.0 5.728436589357596E-5 0.0 11 0.0 0.0 0.0 1.4321091473393989E-4 0.0 12 0.0 0.0 0.0 2.5777964652109184E-4 0.0 13 0.0 0.0 0.0 3.1506401241466775E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 345 0.0 16.52174 2 TTAGACT 35 0.0021709283 16.285715 4 TATAACG 290 0.0 15.724137 2 ATAACGC 305 0.0 15.262296 3 TGCGGAC 205 0.0 14.8292675 9 GCGGACT 210 0.0 14.4761915 10 TACGACC 40 0.0052808486 14.25 4 GGTTTAT 235 0.0 14.148935 1 GTATAAC 330 0.0 14.10606 1 ACAACGC 210 0.0 14.02381 3 GCGTATC 70 7.268749E-6 13.571428 1 TAACGCA 365 0.0 13.534247 4 GTATCAC 505 0.0 12.227722 1 ATAGAAC 70 1.0916517E-4 12.214286 3 CACGCAG 510 0.0 12.107844 5 TCTAGAC 150 1.4551915E-11 12.033333 3 GCGGGAT 230 0.0 11.978261 2 GACTGCG 255 0.0 11.92157 6 CTAGACC 130 2.6193447E-9 11.692307 4 CTGCGGA 260 0.0 11.692307 8 >>END_MODULE