FastQCFastQC Report
Wed 25 May 2016
SRR1295365_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295365_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5385368
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACATGGGGG56350.10463537496416214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGCG551.9615646E-413.81818211
TATAACG3100.012.5645162
ATAACGC3500.011.6714293
GTGGTAT9600.011.3802091
CGCGCTT1604.5474735E-1111.2812512
GGTATCA24500.010.973471
CGAGCGT2200.010.3636366
GTATCAA51400.09.943581
CGTGCGA1201.702311E-59.510
TACGCCG800.00451850159.55
GTATAAA5900.09.51
ACGAGCG2401.8189894E-129.55
GTACGAC2002.6557245E-109.53
TATACTG5700.09.3333335
GTATTAA9300.09.2956991
TAACGCA3500.09.2285714
GTATAAC4250.09.1647051
TACGACC2700.09.1481494
GTTCTAG5350.09.0560751
CTATACC3100.08.8870974
Produced by FastQC (version 0.11.5)