##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295365_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5385368 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.7047509102442 32.0 32.0 33.0 18.0 34.0 2 30.854660071512292 32.0 32.0 33.0 18.0 34.0 3 31.062288965210918 33.0 32.0 33.0 29.0 34.0 4 31.21845879427367 33.0 32.0 33.0 29.0 34.0 5 31.28448566560354 33.0 32.0 33.0 29.0 34.0 6 33.97759038936615 37.0 33.0 37.0 29.0 37.0 7 34.006696292621044 37.0 33.0 37.0 29.0 37.0 8 33.92808365185072 37.0 33.0 37.0 29.0 37.0 9 33.905786939722596 37.0 33.0 37.0 29.0 37.0 10 33.88121034625674 37.0 33.0 37.0 29.0 37.0 11 33.89023034266182 37.0 33.0 37.0 29.0 37.0 12 33.82423689523167 37.0 33.0 37.0 29.0 37.0 13 35.42371421971535 38.0 37.0 38.0 32.0 38.0 14 35.445364179383844 38.0 37.0 38.0 32.0 38.0 15 35.452023520026856 38.0 37.0 38.0 32.0 38.0 16 35.39432737001445 38.0 37.0 38.0 32.0 38.0 17 35.44200024956512 38.0 37.0 38.0 32.0 38.0 18 35.28895796907472 38.0 37.0 38.0 32.0 38.0 19 35.400229845017094 38.0 37.0 38.0 32.0 38.0 20 35.449091872644544 38.0 37.0 38.0 32.0 38.0 21 35.408514701316605 38.0 37.0 38.0 32.0 38.0 22 35.42020266767285 38.0 37.0 38.0 32.0 38.0 23 35.84690944054334 38.0 37.0 39.0 32.0 39.0 24 35.8517612909647 38.0 37.0 39.0 32.0 39.0 25 35.68112931186875 38.0 37.0 39.0 32.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36703347058505287 1101 2 0.09029938183741848 1101 3 0.1763867211080452 1101 4 0.12478618023319754 1101 5 0.27631896408485446 1101 6 0.31552042159457727 1101 7 0.5242922251899458 1101 8 0.4185585104514047 1101 9 0.3115492955979775 1101 10 0.43701248219867495 1101 11 0.2555250525571324 1101 12 0.3642702913773306 1101 13 0.415641738541197 1101 14 0.33459925153747605 1101 15 0.2697591675483295 1101 16 0.5527085210429732 1101 17 0.2889219947865129 1101 18 0.3686333301575502 1101 19 0.27820892337724246 1101 20 0.4741119826588118 1101 21 0.501906412364626 1101 22 0.4015991242827539 1101 23 0.509987893214543 1101 24 0.4283625492107461 1101 25 0.3665796632738605 1102 1 0.3734276295625243 1102 2 0.22924964785660862 1102 3 0.39241696911848223 1102 4 0.1630506257363855 1102 5 0.3252253364572475 1102 6 0.3122538781218722 1102 7 0.485118735583832 1102 8 0.46530780831280083 1102 9 0.5958083172768411 1102 10 0.46249850582953655 1102 11 0.43433865880681566 1102 12 0.5153329987016164 1102 13 0.4828251899540277 1102 14 0.49140692396027674 1102 15 0.5384873613470447 1102 16 0.6341658648110311 1102 17 0.45283400382638206 1102 18 0.594911222843308 1102 19 0.5646913219630818 1102 20 0.5005257837176984 1102 21 0.5093464881771439 1102 22 0.5354750945318898 1102 23 0.4210324736304685 1102 24 0.5786958405850484 1102 25 0.5300933801022865 1103 1 -0.0338672222004206 1103 2 0.1547410790351904 1103 3 0.2767617671758984 1103 4 0.07169287334930274 1103 5 0.19008102067938282 1103 6 0.34511691872012307 1103 7 0.4256797105750607 1103 8 0.47341187331515044 1103 9 0.5457948441973528 1103 10 0.4488673797152245 1103 11 0.42529659849409285 1103 12 0.3261811702590336 1103 13 0.5378812009142706 1103 14 0.4658684831394879 1103 15 0.40933584099869336 1103 16 0.620339938316512 1103 17 0.4148174498924675 1103 18 0.45505198850538875 1103 19 0.38471072486766644 1103 20 0.4744202033839713 1103 21 0.46030029254539073 1103 22 0.29337690951457773 1103 23 0.24565083123856368 1103 24 0.3490273679997884 1103 25 0.30343244123412205 1104 1 0.24161089117718149 1104 2 0.1959567480798725 1104 3 0.22749521889166147 1104 4 0.21861600749972965 1104 5 0.3923032218973965 1104 6 0.4001915033093866 1104 7 0.523832215967218 1104 8 0.5141185121424314 1104 9 0.5321424220428455 1104 10 0.44308114374594965 1104 11 0.3935470011142783 1104 12 0.2626622332373074 1104 13 0.33644990977947486 1104 14 0.34625651996893936 1104 15 0.16948526934206143 1104 16 0.40766340895435604 1104 17 0.39429014703000576 1104 18 0.2533969771417688 1104 19 0.26569941430465605 1104 20 0.27058500599488866 1104 21 0.28406332929862543 1104 22 0.21592290822302118 1104 23 0.26052895059220305 1104 24 0.2835258962484488 1104 25 0.42661958324364235 1105 1 0.0521878761504837 1105 2 0.058417268137407774 1105 3 0.3024676886136568 1105 4 0.2853411849357137 1105 5 0.2489734712447884 1105 6 0.35488162566901593 1105 7 0.5813838573704828 1105 8 0.23279696594124033 1105 9 0.5307905780337023 1105 10 0.46474240111017906 1105 11 0.25609835442257634 1105 12 0.3893054460291907 1105 13 0.40863708441450797 1105 14 0.5338721448638282 1105 15 0.45129522419015444 1105 16 0.4181812727692886 1105 17 0.5956124153084588 1105 18 0.5278280858840532 1105 19 0.4285382327195819 1105 20 0.5215285603319231 1105 21 0.44186292610418576 1105 22 0.47496325714737964 1105 23 0.2549459199533999 1105 24 0.503320328469961 1105 25 0.505597520103521 1106 1 0.2680790176365768 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0.026656023154664865 2110 17 -0.7587960763185606 2110 18 -0.10944137054690373 2110 19 0.007619838334747442 2110 20 -0.056612297669040856 2110 21 0.0505039474178659 2110 22 0.34447156520707267 2110 23 -0.057825577947205886 2110 24 -0.16786506453506433 2110 25 0.275626652854676 2111 1 0.15821473109296846 2111 2 0.17212151407791865 2111 3 0.013119588871145282 2111 4 -0.07447399525698373 2111 5 -0.038249840691715775 2111 6 -0.07120188114219417 2111 7 -0.01203222722576669 2111 8 0.043406043599041766 2111 9 -0.22604680923151932 2111 10 -0.028729687543027183 2111 11 -0.08063071379593367 2111 12 -0.3701026626919486 2111 13 -0.32318982867870716 2111 14 -0.39043873611198876 2111 15 -0.26083826533610477 2111 16 -0.2722881354076563 2111 17 -1.0570617454981104 2111 18 -0.5384385814920094 2111 19 -0.08523922443814769 2111 20 0.09315552741961142 2111 21 0.07312752247365495 2111 22 0.12080288404174411 2111 23 -0.07050391726838967 2111 24 -0.07012496706249749 2111 25 0.2578202331351065 2112 1 -0.18732548228289403 2112 2 -0.259519616124269 2112 3 -0.06602617019322565 2112 4 -0.2503806219258209 2112 5 -0.2725032784909409 2112 6 -0.32401585408592126 2112 7 -0.2165145635780874 2112 8 0.014800625832407377 2112 9 -0.03260821645060474 2112 10 0.09972592335684283 2112 11 -0.03563154665997814 2112 12 -0.38105156369688586 2112 13 -0.15942766320372215 2112 14 -0.6423602685670531 2112 15 -0.4614462503853147 2112 16 -0.1832392020384006 2112 17 -0.10334106316690139 2112 18 -0.5156860934685668 2112 19 -0.02906956268363814 2112 20 0.006026734278599122 2112 21 -0.2634525227644531 2112 22 -0.02404117354106461 2112 23 -0.2747359977919004 2112 24 -0.32010636827032357 2112 25 -0.49312598262421403 2113 1 -0.3343829615962157 2113 2 -0.28428227478776336 2113 3 -0.20239131899575113 2113 4 -0.2538297840778121 2113 5 -0.6166085814734039 2113 6 -0.43098834274798037 2113 7 -0.48870072934005293 2113 8 -0.42682517897812744 2113 9 -0.19884443803913854 2113 10 0.0281671513289794 2113 11 -0.08535455146577675 2113 12 -0.29742167606821823 2113 13 -0.15749440150815275 2113 14 -0.5300360788037324 2113 15 -0.4740023289831825 2113 16 -0.23299268994348665 2113 17 -0.20057918626390148 2113 18 -0.36073527015275175 2113 19 -0.2874115897890803 2113 20 -0.4228966821108173 2113 21 -0.39812925189296067 2113 22 -0.21394417465756987 2113 23 -0.5622003499001949 2113 24 -0.4455110721533728 2113 25 -0.995226267632674 2114 1 -0.3842094510400891 2114 2 -0.25584359116149713 2114 3 -0.44095563383792324 2114 4 -0.32890009630523664 2114 5 -0.403955715462498 2114 6 -0.5336349712558288 2114 7 -0.40752726323201216 2114 8 -0.26993156490912185 2114 9 -0.08732665157052821 2114 10 0.062020797765235613 2114 11 -0.5079463545773351 2114 12 -0.14258066334510033 2114 13 -0.08361283569928446 2114 14 -0.3040060333289958 2114 15 -0.4561915025486343 2114 16 -0.1926331269366912 2114 17 -0.1534574813241676 2114 18 -0.23878756978336213 2114 19 -0.45122618731657127 2114 20 -0.4547241766111654 2114 21 -0.25363728560848386 2114 22 -0.23747217241307794 2114 23 -0.34188249076587596 2114 24 -0.1620399979170699 2114 25 -0.6112515816487303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 8.0 15 59.0 16 546.0 17 2400.0 18 6509.0 19 14375.0 20 25103.0 21 36973.0 22 48631.0 23 59295.0 24 69109.0 25 78708.0 26 89039.0 27 99116.0 28 110846.0 29 124347.0 30 140233.0 31 160944.0 32 192848.0 33 251802.0 34 388338.0 35 866469.0 36 2373705.0 37 245965.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.58215297450425 17.87500872735159 12.573068358559711 24.969769939584445 2 17.471842221367233 19.579441924860102 37.70037627883554 25.248339574937127 3 18.67812933118034 24.393727596702767 29.413885922001988 27.5142571501149 4 14.026878014650066 16.824495559077857 36.23397695384977 32.91464947242231 5 14.675171687431574 35.529586836034234 33.42752064482873 16.367720831705466 6 31.556840683867843 35.298070623957365 18.388882616749683 14.756206075425116 7 28.455548441629247 30.41929539448372 22.397578029950786 18.72757813393625 8 26.27957829437097 33.3095342788088 20.82154831387567 19.589339112944558 9 26.24063945119442 16.4289608435301 20.402486886690006 36.927912818585476 10 17.08553621590948 26.809031434806315 31.955532101056043 24.149900248228164 11 33.602141952044875 22.733581066326387 24.06583914042643 19.59843784120231 12 23.962280757786655 24.249020679738134 29.370509127695637 22.418189434779574 13 28.083911814382972 21.207668631001635 25.884712056817662 24.823707497797738 14 23.200772909112246 20.739993998553118 26.152177529929244 29.907055562405393 15 24.3584468136625 27.76239246788706 23.240547349781853 24.638613368668587 16 24.31922943798827 27.063294467527566 24.103589578279518 24.51388651620465 17 23.344105732421628 26.883325336355846 25.82343490732667 23.94913402389586 18 23.253768359005363 26.831406878787114 26.501290162529283 23.41353459967824 19 24.40752423975483 25.98307116616729 26.410544274783078 23.198860319294802 20 24.68770936359409 26.190930684774 25.973953869076354 23.14740608255555 21 24.876888636022645 25.62292864665887 25.73785115520425 23.762331562114234 22 24.59963738782568 25.6982066963669 25.83570890605805 23.86644700974938 23 24.56400379695501 25.863673568825753 25.492835401406182 24.07948723281306 24 23.774679836178326 26.096192497894293 25.882576641002064 24.246551024925317 25 24.25076615005697 25.47333812656814 26.14987499461504 24.126020728759855 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 908.0 1 908.0 2 1110.0 3 1312.0 4 1312.0 5 1312.0 6 3397.5 7 5483.0 8 5483.0 9 5483.0 10 8010.0 11 10537.0 12 10537.0 13 10537.0 14 17066.0 15 23595.0 16 23595.0 17 23595.0 18 39876.5 19 56158.0 20 56158.0 21 56158.0 22 84285.5 23 112413.0 24 112413.0 25 112413.0 26 153082.0 27 193751.0 28 193751.0 29 193751.0 30 240405.5 31 287060.0 32 287060.0 33 287060.0 34 339068.5 35 391077.0 36 391077.0 37 391077.0 38 445138.0 39 499199.0 40 499199.0 41 499199.0 42 550463.5 43 601728.0 44 601728.0 45 601728.0 46 622553.5 47 643379.0 48 643379.0 49 643379.0 50 653531.5 51 663684.0 52 663684.0 53 663684.0 54 648293.0 55 632902.0 56 632902.0 57 632902.0 58 572598.0 59 512294.0 60 512294.0 61 512294.0 62 437654.5 63 363015.0 64 363015.0 65 363015.0 66 286010.5 67 209006.0 68 209006.0 69 209006.0 70 157191.0 71 105376.0 72 105376.0 73 105376.0 74 75884.5 75 46393.0 76 46393.0 77 46393.0 78 32240.5 79 18088.0 80 18088.0 81 18088.0 82 12086.0 83 6084.0 84 6084.0 85 6084.0 86 3749.0 87 1414.0 88 1414.0 89 1414.0 90 884.5 91 355.0 92 355.0 93 355.0 94 227.0 95 99.0 96 99.0 97 99.0 98 78.5 99 58.0 100 58.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5385368.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.23013879854534 #Duplication Level Percentage of deduplicated Percentage of total 1 74.30688338114089 26.921486985860565 2 10.961144305695642 7.94247559172476 3 4.284300650809471 4.656624217005758 4 2.280779296306809 3.3053180189617706 5 1.465271482814313 2.654349459995645 6 0.9844077435127262 2.1399137509097335 7 0.7535570926320113 1.9111034641080242 8 0.5992947679195265 1.737002609837317 9 0.46984445837158234 1.5320276946476812 >10 3.3392562979963896 23.840800881967958 >50 0.35491532247518287 8.85788090752267 >100 0.19396560133971047 12.366124242584386 >500 0.0054020807118033065 1.294647205419675 >1k 9.260699437077501E-4 0.7352096883219637 >5k 5.144833020589286E-5 0.10503528113203142 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACATGGGGG 5635 0.10463537496416214 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.8568833179088225E-5 0.0 5 1.8568833179088225E-5 0.0 0.0 1.8568833179088225E-5 0.0 6 1.8568833179088225E-5 0.0 0.0 1.8568833179088225E-5 0.0 7 1.8568833179088225E-5 0.0 0.0 1.8568833179088225E-5 0.0 8 1.8568833179088225E-5 0.0 0.0 3.713766635817645E-5 0.0 9 1.8568833179088225E-5 0.0 0.0 7.42753327163529E-5 0.0 10 1.8568833179088225E-5 0.0 0.0 1.485506654327058E-4 0.0 11 1.8568833179088225E-5 0.0 0.0 2.0425716496997047E-4 0.0 12 1.8568833179088225E-5 0.0 0.0 2.4139483132814695E-4 0.0 13 1.8568833179088225E-5 0.0 0.0 2.7853249768632337E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAACGCG 55 1.9615646E-4 13.818182 11 TATAACG 310 0.0 12.564516 2 ATAACGC 350 0.0 11.671429 3 GTGGTAT 960 0.0 11.380209 1 CGCGCTT 160 4.5474735E-11 11.28125 12 GGTATCA 2450 0.0 10.97347 1 CGAGCGT 220 0.0 10.363636 6 GTATCAA 5140 0.0 9.94358 1 CGTGCGA 120 1.702311E-5 9.5 10 TACGCCG 80 0.0045185015 9.5 5 GTATAAA 590 0.0 9.5 1 ACGAGCG 240 1.8189894E-12 9.5 5 GTACGAC 200 2.6557245E-10 9.5 3 TATACTG 570 0.0 9.333333 5 GTATTAA 930 0.0 9.295699 1 TAACGCA 350 0.0 9.228571 4 GTATAAC 425 0.0 9.164705 1 TACGACC 270 0.0 9.148149 4 GTTCTAG 535 0.0 9.056075 1 CTATACC 310 0.0 8.887097 4 >>END_MODULE